7o0k - Revision history

OCA at 12:42, 1 February 2024

2024-02-01T12:42:49Z

←Older revision		Revision as of 12:42, 1 February 2024
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	==Crystal structure of recombinant chichen liver Bile Acid Binding Protein (cL-BABP) in complex with cholic acid==		==Crystal structure of recombinant chichen liver Bile Acid Binding Protein (cL-BABP) in complex with cholic acid==
-	<StructureSection load='7o0k' size='340' side='right'caption='[[7o0k]]' scene=''>	+	<StructureSection load='7o0k' size='340' side='right'caption='[[7o0k]], [[Resolution\|resolution]] 1.83Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7O0K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7O0K FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7o0k]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7O0K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7O0K FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7o0k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7o0k OCA], [https://pdbe.org/7o0k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7o0k RCSB], [https://www.ebi.ac.uk/pdbsum/7o0k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7o0k ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.83Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CHD:CHOLIC+ACID'>CHD</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7o0k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7o0k OCA], [https://pdbe.org/7o0k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7o0k RCSB], [https://www.ebi.ac.uk/pdbsum/7o0k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7o0k ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/FABPL_CHICK FABPL_CHICK] Binds free fatty acids and their coenzyme A derivatives, bilirubin, and some other small molecules in the cytoplasm. May be involved in intracellular lipid transport. Binds 2 molecules of cholate per subunit.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Bile acids (BAs) are hydroxylated steroids derived from cholesterol that act at the intestinal level to facilitate the absorption of several nutrients and also play a role as signaling molecules. In the liver of various vertebrates, the trafficking of BAs is mediated by bile acid-binding proteins (L-BABPs). The ability to host hydrophobic or amphipathic molecules makes BABPs suitable for the distribution of a variety of physiological and exogenous substances. Thus, BABPs have been proposed as drug carriers, and more recently, they have also been employed to develop innovative nanotechnology and biotechnology systems. Here, we report an efficient protocol for the production, purification, and crystallization of chicken liver BABP (cL-BABP). By means of target expression as His(6)-tag cL-BABP, we obtained a large amount of pure and homogeneous proteins through a simple purification procedure relying on affinity chromatography. The recombinant cL-BABP showed a raised propensity to crystallize, allowing us to obtain its structure at high resolution and, in turn, assess the structural conservation of the recombinant cL-BABP with respect to the liver-extracted protein. The results support the use of recombinant cL-BABP for the development of drug carriers, nanotechnologies, and innovative synthetic photoswitch systems.
		+
		+	Validation of Recombinant Chicken Liver Bile Acid Binding Protein as a Tool for Cholic Acid Hosting.,Tassone G, Orlandini M, Olivucci M, Pozzi C Biomolecules. 2021 Apr 27;11(5). pii: biom11050645. doi: 10.3390/biom11050645. PMID:33925706<ref>PMID:33925706</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7o0k" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Fatty acid-binding protein 3D structures\|Fatty acid-binding protein 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Gallus gallus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Pozzi C]]		[[Category: Pozzi C]]
	[[Category: Tassone G]]		[[Category: Tassone G]]

OCA at 09:23, 12 May 2021

2021-05-12T09:23:32Z

←Older revision		Revision as of 09:23, 12 May 2021
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-	'''Unreleased structure'''

-	~~The entry 7o0k is ON HOLD until Paper Publication~~	+	==Crystal structure of recombinant chichen liver Bile Acid Binding Protein (cL-BABP) in complex with cholic acid==
-		+	<StructureSection load='7o0k' size='340' side='right'caption='[[7o0k]]' scene=''>
-	~~Authors: Tassone, G., Pozzi, C.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7O0K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7O0K FirstGlance]. <br>
-	~~Description:~~ Crystal structure of recombinant chichen liver Bile Acid Binding Protein (cL-BABP) in complex with cholic acid	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7o0k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7o0k OCA], [https://pdbe.org/7o0k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7o0k RCSB], [https://www.ebi.ac.uk/pdbsum/7o0k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7o0k ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[~~Category~~: ~~Tassone~~, G]]	+	__TOC__
-	[[Category: Pozzi, C]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Pozzi C]]
		+	[[Category: Tassone G]]

OCA at 05:47, 14 April 2021

2021-04-14T05:47:44Z

←Older revision		Revision as of 05:47, 14 April 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7o0k is ON HOLD	+	The entry 7o0k is ON HOLD until Paper Publication

	Authors: Tassone, G., Pozzi, C.		Authors: Tassone, G., Pozzi, C.

OCA: Protected "7o0k" [edit=sysop:move=sysop]

2021-04-07T04:48:37Z

Protected "7o0k" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:48, 7 April 2021

OCA: New page: '''Unreleased structure''' The entry 7o0k is ON HOLD Authors: Tassone, G., Pozzi, C. Description: Crystal structure of recombinant chichen liver Bile Acid Binding Protein (cL-BABP) in ...

2021-04-07T04:48:35Z

New page: '''Unreleased structure''' The entry 7o0k is ON HOLD Authors: Tassone, G., Pozzi, C. Description: Crystal structure of recombinant chichen liver Bile Acid Binding Protein (cL-BABP) in ...

New page

'''Unreleased structure'''

The entry 7o0k is ON HOLD

Authors: Tassone, G., Pozzi, C.

Description: Crystal structure of recombinant chichen liver Bile Acid Binding Protein (cL-BABP) in complex with cholic acid
[[Category: Unreleased Structures]]
[[Category: Tassone, G]]
[[Category: Pozzi, C]]