OCA at 06:23, 26 October 2022

2022-10-26T06:23:10Z

←Older revision		Revision as of 06:23, 26 October 2022
Line 1:		Line 1:

-	====	+	==ABC transporter NosDFY, nucleotide-free in GDN, R-domain 2==
-	<StructureSection load='7o10' size='340' side='right'caption='[[7o10]]' scene=''>	+	<StructureSection load='7o10' size='340' side='right'caption='[[7o10]], [[Resolution\|resolution]] 3.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7o10]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_stutzeri_ATCC_14405_=_CCUG_16156 Pseudomonas stutzeri ATCC 14405 = CCUG 16156]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7O10 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7O10 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7o10 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7o10 OCA], [https://pdbe.org/7o10 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7o10 RCSB], [https://www.ebi.ac.uk/pdbsum/7o10 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7o10 ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7o10 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7o10 OCA], [https://pdbe.org/7o10 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7o10 RCSB], [https://www.ebi.ac.uk/pdbsum/7o10 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7o10 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/NOSD_PSEST NOSD_PSEST] Required for the assembly of the copper chromophores of nitrous oxide reductase (PubMed:2170125). Could be part of the ABC transporter complex NosDFY (Probable).<ref>PMID:2170125</ref> <ref>PMID:12618453</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Emissions of the critical ozone-depleting and greenhouse gas nitrous oxide (N2O) from soils and industrial processes have increased considerably over the last decades(1-3). As the final step of bacterial denitrification, N2O is reduced to chemically inert N2 (refs. (1,4)) in a reaction that is catalysed by the copper-dependent nitrous oxide reductase (N2OR) (ref. (5)). The assembly of its unique [4Cu:2S] active site cluster CuZ requires both the ATP-binding-cassette (ABC) complex NosDFY and the membrane-anchored copper chaperone NosL (refs. (4,6)). Here we report cryo-electron microscopy structures of Pseudomonas stutzeri NosDFY and its complexes with NosL and N2OR, respectively. We find that the periplasmic NosD protein contains a binding site for a Cu(+) ion and interacts specifically with NosL in its nucleotide-free state, whereas its binding to N2OR requires a conformational change that is triggered by ATP binding. Mutually exclusive structures of NosDFY in complex with NosL and with N2OR reveal a sequential metal-trafficking and assembly pathway for a highly complex copper site. Within this pathway, NosDFY acts as a mechanical energy transducer rather than as a transporter. It links ATP hydrolysis in the cytoplasm to a conformational transition of the NosD subunit in the periplasm, which is required for NosDFY to switch its interaction partner so that copper ions are handed over from the chaperone NosL to the enzyme N2OR.
		+
		+	Molecular interplay of an assembly machinery for nitrous oxide reductase.,Muller C, Zhang L, Zipfel S, Topitsch A, Lutz M, Eckert J, Prasser B, Chami M, Lu W, Du J, Einsle O Nature. 2022 Aug;608(7923):626-631. doi: 10.1038/s41586-022-05015-2. Epub 2022, Jul 27. PMID:35896743<ref>PMID:35896743</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7o10" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Pseudomonas stutzeri ATCC 14405 = CCUG 16156]]
		+	[[Category: Du J]]
		+	[[Category: Einsle O]]
		+	[[Category: Lu W]]
		+	[[Category: Mueller C]]
		+	[[Category: Zhang L]]

OCA at 09:45, 13 April 2022

2022-04-13T09:45:46Z

←Older revision		Revision as of 09:45, 13 April 2022
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7o10 ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7o10' size='340' side='right'caption='[[7o10]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7o10 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7o10 OCA], [https://pdbe.org/7o10 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7o10 RCSB], [https://www.ebi.ac.uk/pdbsum/7o10 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7o10 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 07:32, 5 May 2021

2021-05-05T07:32:45Z

←Older revision		Revision as of 07:32, 5 May 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7o10 is ON HOLD	+	The entry 7o10 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7o10" [edit=sysop:move=sysop]

2021-04-07T04:50:04Z

Protected "7o10" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:50, 7 April 2021

OCA: New page: '''Unreleased structure''' The entry 7o10 is ON HOLD Authors: Description: Category: Unreleased Structures

2021-04-07T04:50:01Z

New page: '''Unreleased structure''' The entry 7o10 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7o10 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7o10 - Revision history

OCA at 06:23, 26 October 2022

OCA at 09:45, 13 April 2022

OCA at 07:32, 5 May 2021

OCA: Protected "7o10" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7o10 is ON HOLD Authors: Description: Category: Unreleased Structures