7o35 - Revision history

OCA at 12:44, 1 February 2024

2024-02-01T12:44:04Z

←Older revision		Revision as of 12:44, 1 February 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7o35]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Severe_acute_respiratory_syndrome_coronavirus_2 Severe acute respiratory syndrome coronavirus 2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7O35 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7O35 FirstGlance]. <br>		<table><tr><td colspan='2'>[[7o35]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Severe_acute_respiratory_syndrome_coronavirus_2 Severe acute respiratory syndrome coronavirus 2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7O35 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7O35 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.8Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7o35 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7o35 OCA], [https://pdbe.org/7o35 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7o35 RCSB], [https://www.ebi.ac.uk/pdbsum/7o35 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7o35 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7o35 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7o35 OCA], [https://pdbe.org/7o35 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7o35 RCSB], [https://www.ebi.ac.uk/pdbsum/7o35 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7o35 ProSAT]</span></td></tr>
	</table>		</table>
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	</div>		</div>
	<div class="pdbe-citations 7o35" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 7o35" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Nucleoprotein 3D structures\|Nucleoprotein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 06:23, 26 October 2022

2022-10-26T06:23:22Z

←Older revision		Revision as of 06:23, 26 October 2022
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	==Crystal Structure of SARS-CoV-2 N-CTD in complex with GTP (I)==		==Crystal Structure of SARS-CoV-2 N-CTD in complex with GTP (I)==
-	<StructureSection load='7o35' size='340' side='right'caption='[[7o35]]' scene=''>	+	<StructureSection load='7o35' size='340' side='right'caption='[[7o35]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7O35 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7O35 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7o35]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Severe_acute_respiratory_syndrome_coronavirus_2 Severe acute respiratory syndrome coronavirus 2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7O35 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7O35 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7o35 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7o35 OCA], [https://pdbe.org/7o35 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7o35 RCSB], [https://www.ebi.ac.uk/pdbsum/7o35 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7o35 ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7o35 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7o35 OCA], [https://pdbe.org/7o35 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7o35 RCSB], [https://www.ebi.ac.uk/pdbsum/7o35 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7o35 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/NCAP_SARS2 NCAP_SARS2] Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The SARS-CoV-2 nucleocapsid protein (N) is responsible for RNA binding. Here we report the crystal structure of the C-terminal domain (N(CTD)) in open and closed conformations and in complex with guanine triphosphate, GTP. The crystal structure and biochemical studies reveal a specific interaction between the guanine, a nucleotide enriched in the packaging signals regions of coronaviruses, and a highly conserved tryptophan residue (W330). In addition, EMSA assays with SARS-CoV-2 derived RNA hairpin loops from a putative viral packaging sequence showed the preference interaction of the N-CTD to RNA oligonucleotides containing G and the loss of the specificity in the mutant W330A. Here we propose that this interaction may facilitate the viral assembly process. In summary, we have identified a specific guanine-binding pocket in the N protein that may be used to design viral assembly inhibitors.
		+
		+	Identification of a guanine-specific pocket in the protein N of SARS-CoV-2.,Rafael Ciges-Tomas J, Franco ML, Vilar M Commun Biol. 2022 Jul 16;5(1):711. doi: 10.1038/s42003-022-03647-8. PMID:35842466<ref>PMID:35842466</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7o35" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Severe acute respiratory syndrome coronavirus 2]]
	[[Category: Ciges-Tomas JR]]		[[Category: Ciges-Tomas JR]]
	[[Category: Vilar M]]		[[Category: Vilar M]]

OCA at 09:46, 13 April 2022

2022-04-13T09:46:08Z

←Older revision		Revision as of 09:46, 13 April 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7o35 ~~is ON HOLD until Paper Publication~~	+	==Crystal Structure of SARS-CoV-2 N-CTD in complex with GTP (I)==
-		+	<StructureSection load='7o35' size='340' side='right'caption='[[7o35]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7O35 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7O35 FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7o35 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7o35 OCA], [https://pdbe.org/7o35 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7o35 RCSB], [https://www.ebi.ac.uk/pdbsum/7o35 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7o35 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Ciges-Tomas JR]]
		+	[[Category: Vilar M]]

OCA: Protected "7o35" [edit=sysop:move=sysop]

2021-04-07T04:58:26Z

Protected "7o35" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:58, 7 April 2021

OCA: New page: '''Unreleased structure''' The entry 7o35 is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

2021-04-07T04:58:25Z

New page: '''Unreleased structure''' The entry 7o35 is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7o35 is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]