7o3b - Revision history

OCA at 12:44, 1 February 2024

2024-02-01T12:44:09Z

←Older revision		Revision as of 12:44, 1 February 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7o3b]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Camelidae_mixed_library Camelidae mixed library] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7O3B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7O3B FirstGlance]. <br>		<table><tr><td colspan='2'>[[7o3b]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Camelidae_mixed_library Camelidae mixed library] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7O3B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7O3B FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7o3b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7o3b OCA], [https://pdbe.org/7o3b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7o3b RCSB], [https://www.ebi.ac.uk/pdbsum/7o3b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7o3b ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.4Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7o3b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7o3b OCA], [https://pdbe.org/7o3b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7o3b RCSB], [https://www.ebi.ac.uk/pdbsum/7o3b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7o3b ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==

OCA at 10:07, 21 December 2022

2022-12-21T10:07:44Z

←Older revision		Revision as of 10:07, 21 December 2022
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-	====	+	==Crystal structure of the TTBK2-CEP164 complex bound to a camelid nanobody==
-	<StructureSection load='7o3b' size='340' side='right'caption='[[7o3b]]' scene=''>	+	<StructureSection load='7o3b' size='340' side='right'caption='[[7o3b]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7o3b]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Camelidae_mixed_library Camelidae mixed library] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7O3B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7O3B FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7o3b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7o3b OCA], [https://pdbe.org/7o3b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7o3b RCSB], [https://www.ebi.ac.uk/pdbsum/7o3b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7o3b ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7o3b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7o3b OCA], [https://pdbe.org/7o3b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7o3b RCSB], [https://www.ebi.ac.uk/pdbsum/7o3b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7o3b ProSAT]</span></td></tr>
	</table>		</table>
		+	== Disease ==
		+	[https://www.uniprot.org/uniprot/CE164_HUMAN CE164_HUMAN] Senior-Loken syndrome. The disease is caused by variants affecting the gene represented in this entry.[https://www.uniprot.org/uniprot/TTBK2_HUMAN TTBK2_HUMAN] Spinocerebellar ataxia type 11. The disease is caused by mutations affecting the gene represented in this entry.
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/CE164_HUMAN CE164_HUMAN] Plays a role in microtubule organization and/or maintenance for the formation of primary cilia (PC), a microtubule-based structure that protrudes from the surface of epithelial cells. Plays a critical role in G2/M checkpoint and nuclear divisions. A key player in the DNA damage-activated ATR/ATM signaling cascade since it is required for the proper phosphorylation of H2AX, RPA, CHEK2 and CHEK1. Plays a critical role in chromosome segregation, acting as a mediator required for the maintenance of genomic stability through modulation of MDC1, RPA and CHEK1.<ref>PMID:17954613</ref> <ref>PMID:18283122</ref> <ref>PMID:23348840</ref> [https://www.uniprot.org/uniprot/TTBK2_HUMAN TTBK2_HUMAN] Serine/threonine kinase that acts as a key regulator of ciliogenesis: controls the initiation of ciliogenesis by binding to the distal end of the basal body and promoting the removal of CCP110, which caps the mother centriole, leading to the recruitment of IFT proteins, which build the ciliary axoneme. Has some substrate preference for proteins that are already phosphorylated on a Tyr residue at the +2 position relative to the phosphorylation site. Able to phosphorylate tau on serines in vitro.<ref>PMID:21548880</ref> <ref>PMID:23141541</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Cilia formation is essential for human life. One of the earliest events in the ciliogenesis program is the recruitment of tau-tubulin kinase 2 (TTBK2) by the centriole distal appendage component CEP164. Due to the lack of high-resolution structural information on this complex, it is unclear how it is affected in human ciliopathies such as nephronophthisis. Furthermore, it is poorly understood if binding to CEP164 influences TTBK2 activities. Here, we present a detailed biochemical, structural, and functional analysis of the CEP164-TTBK2 complex and demonstrate how it is compromised by two ciliopathic mutations in CEP164. Moreover, we also provide insights into how binding to CEP164 is coordinated with TTBK2 activities. Together, our data deepen our understanding of a crucial step in cilia formation and will inform future studies aimed at restoring CEP164 functionality in a debilitating human ciliopathy.
		+
		+	Molecular mechanisms underlying the role of the centriolar CEP164-TTBK2 complex in ciliopathies.,Rosa E Silva I, Bino L, Johnson CM, Rutherford TJ, Neuhaus D, Andreeva A, Cajanek L, van Breugel M Structure. 2021 Sep 7. pii: S0969-2126(21)00302-6. doi:, 10.1016/j.str.2021.08.007. PMID:34499853<ref>PMID:34499853</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7o3b" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Camelidae mixed library]]
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: E Silva IR]]
		+	[[Category: Van Breugel M]]

OCA at 09:28, 15 September 2021

2021-09-15T09:28:11Z

←Older revision		Revision as of 09:28, 15 September 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7o3b ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7o3b' size='340' side='right'caption='[[7o3b]]' scene=''>
-	~~Authors~~: ~~e Silva, I~~.R.~~, van Breugel, M~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description: Crystal structure of the TTBK2~~-~~CEP164 complex bound to~~ a ~~camelid nanobody~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7o3b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7o3b OCA], [https://pdbe.org/7o3b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7o3b RCSB], [https://www.ebi.ac.uk/pdbsum/7o3b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7o3b ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[Category: ~~Van Breugel, M~~]]	+	__TOC__
-	[[Category: ~~E Silva, I.R~~]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 08:18, 12 May 2021

2021-05-12T08:18:29Z

←Older revision		Revision as of 08:18, 12 May 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7o3b is ON HOLD	+	The entry 7o3b is ON HOLD until Paper Publication

	Authors: e Silva, I.R., van Breugel, M.		Authors: e Silva, I.R., van Breugel, M.

OCA at 05:48, 14 April 2021

2021-04-14T05:48:04Z

←Older revision		Revision as of 05:48, 14 April 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7o3b is ON HOLD ~~until sometime in the future~~	+	The entry 7o3b is ON HOLD

	Authors: e Silva, I.R., van Breugel, M.		Authors: e Silva, I.R., van Breugel, M.

OCA: Protected "7o3b" [edit=sysop:move=sysop]

2021-04-07T04:59:08Z

Protected "7o3b" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:59, 7 April 2021

OCA: New page: '''Unreleased structure''' The entry 7o3b is ON HOLD until sometime in the future Authors: e Silva, I.R., van Breugel, M. Description: Crystal structure of the TTBK2-CEP164 complex bou...

2021-04-07T04:59:06Z

New page: '''Unreleased structure''' The entry 7o3b is ON HOLD until sometime in the future Authors: e Silva, I.R., van Breugel, M. Description: Crystal structure of the TTBK2-CEP164 complex bou...

New page

'''Unreleased structure'''

The entry 7o3b is ON HOLD until sometime in the future

Authors: e Silva, I.R., van Breugel, M.

Description: Crystal structure of the TTBK2-CEP164 complex bound to a camelid nanobody
[[Category: Unreleased Structures]]
[[Category: Van Breugel, M]]
[[Category: E Silva, I.R]]