OCA at 11:02, 15 November 2023

2023-11-15T11:02:35Z

←Older revision		Revision as of 11:02, 15 November 2023
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-	====	+	==Crystal structure of human legumain in complex with Gly-Ser-Asn peptide==
-	<StructureSection load='7o50' size='340' side='right'caption='[[7o50]]' scene=''>	+	<StructureSection load='7o50' size='340' side='right'caption='[[7o50]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7o50]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7O50 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7O50 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7o50 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7o50 OCA], [https://pdbe.org/7o50 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7o50 RCSB], [https://www.ebi.ac.uk/pdbsum/7o50 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7o50 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SNN:L-3-AMINOSUCCINIMIDE'>SNN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7o50 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7o50 OCA], [https://pdbe.org/7o50 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7o50 RCSB], [https://www.ebi.ac.uk/pdbsum/7o50 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7o50 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/LGMN_HUMAN LGMN_HUMAN] Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. May be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Protein modification by enzymatic breaking and forming of peptide bonds significantly expands the repertoire of genetically encoded protein sequences. The dual protease-ligase legumain exerts the two opposing activities within a single protein scaffold. Primarily localized to the endolysosomal system, legumain represents a key enzyme in the generation of antigenic peptides for subsequent presentation on the MHCII complex. Here we show that human legumain catalyzes the ligation and cyclization of linear peptides at near-neutral pH conditions, where legumain is intrinsically unstable. Conformational stabilization significantly enhanced legumain's ligase activity, which further benefited from engineering the prime substrate recognition sites for improved affinity. Additionally, we provide evidence that specific legumain activation states allow for differential regulation of its activities. Together these results set the basis for engineering legumain proteases and ligases with applications in biotechnology and drug development.
		+
		+	The Peptide Ligase Activity of Human Legumain Depends on Fold Stabilization and Balanced Substrate Affinities.,Dall E, Stanojlovic V, Demir F, Briza P, Dahms SO, Huesgen PF, Cabrele C, Brandstetter H ACS Catal. 2021 Oct 1;11(19):11885-11896. doi: 10.1021/acscatal.1c02057. Epub , 2021 Sep 10. PMID:34621593<ref>PMID:34621593</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7o50" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Synthetic construct]]
		+	[[Category: Brandstetter H]]
		+	[[Category: Dall E]]

OCA at 06:52, 22 September 2021

2021-09-22T06:52:43Z

←Older revision		Revision as of 06:52, 22 September 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7o50 ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7o50' size='340' side='right'caption='[[7o50]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7o50 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7o50 OCA], [https://pdbe.org/7o50 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7o50 RCSB], [https://www.ebi.ac.uk/pdbsum/7o50 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7o50 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 06:35, 21 April 2021

2021-04-21T06:35:47Z

←Older revision		Revision as of 06:35, 21 April 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7o50 is ON HOLD	+	The entry 7o50 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7o50" [edit=sysop:move=sysop]

2021-04-14T05:50:53Z

Protected "7o50" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:50, 14 April 2021

OCA: New page: '''Unreleased structure''' The entry 7o50 is ON HOLD Authors: Description: Category: Unreleased Structures

2021-04-14T05:50:52Z

New page: '''Unreleased structure''' The entry 7o50 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7o50 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7o50 - Revision history

OCA at 11:02, 15 November 2023

OCA at 06:52, 22 September 2021

OCA at 06:35, 21 April 2021

OCA: Protected "7o50" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7o50 is ON HOLD Authors: Description: Category: Unreleased Structures