OCA at 12:44, 1 February 2024

2024-02-01T12:44:53Z

←Older revision		Revision as of 12:44, 1 February 2024
Line 1:		Line 1:

-	====	+	==Crystal structure of the carbonic anhydrase-like domain of CcmM in complex with the C-terminal 17 residues of CcaA from Synechococcus elongatus (strain PCC 7942)==
-	<StructureSection load='7o54' size='340' side='right'caption='[[7o54]]' scene=''>	+	<StructureSection load='7o54' size='340' side='right'caption='[[7o54]], [[Resolution\|resolution]] 1.63Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7o54]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Synechococcus_elongatus_PCC_7942_=_FACHB-805 Synechococcus elongatus PCC 7942 = FACHB-805]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7O54 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7O54 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7o54 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7o54 OCA], [https://pdbe.org/7o54 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7o54 RCSB], [https://www.ebi.ac.uk/pdbsum/7o54 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7o54 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.63Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7o54 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7o54 OCA], [https://pdbe.org/7o54 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7o54 RCSB], [https://www.ebi.ac.uk/pdbsum/7o54 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7o54 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/CCMM_SYNE7 CCMM_SYNE7] The presence of two potential DNA-binding regions suggests this protein may be a transcriptional regulator.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Carboxysomes in cyanobacteria enclose the enzymes Rubisco and carbonic anhydrase to optimize photosynthetic carbon fixation. Understanding carboxysome assembly has implications in agricultural biotechnology. Here we analyzed the role of the scaffolding protein CcmM of the beta-cyanobacterium Synechococcus elongatus PCC 7942 in sequestrating the hexadecameric Rubisco and the tetrameric carbonic anhydrase, CcaA. We find that the trimeric CcmM, consisting of gammaCAL oligomerization domains and linked small subunit-like (SSUL) modules, plays a central role in mediation of pre-carboxysome condensate formation through multivalent, cooperative interactions. The gammaCAL domains interact with the C-terminal tails of the CcaA subunits and additionally mediate a head-to-head association of CcmM trimers. Interestingly, SSUL modules, besides their known function in recruiting Rubisco, also participate in intermolecular interactions with the gammaCAL domains, providing further valency for network formation. Our findings reveal the mechanism by which CcmM functions as a central organizer of the pre-carboxysome multiprotein matrix, concentrating the core components Rubisco and CcaA before beta-carboxysome shell formation.
		+
		+	Scaffolding protein CcmM directs multiprotein phase separation in beta-carboxysome biogenesis.,Zang K, Wang H, Hartl FU, Hayer-Hartl M Nat Struct Mol Biol. 2021 Nov;28(11):909-922. doi: 10.1038/s41594-021-00676-5., Epub 2021 Nov 10. PMID:34759380<ref>PMID:34759380</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7o54" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Z-~~disk~~]]	+	[[Category: Synechococcus elongatus PCC 7942 = FACHB-805]]
		+	[[Category: Hartl FU]]
		+	[[Category: Hayer-Hartl M]]
		+	[[Category: Wang H]]
		+	[[Category: Zang K]]

OCA at 06:16, 10 November 2021

2021-11-10T06:16:04Z

←Older revision		Revision as of 06:16, 10 November 2021
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7o54 ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7o54' size='340' side='right'caption='[[7o54]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7o54 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7o54 OCA], [https://pdbe.org/7o54 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7o54 RCSB], [https://www.ebi.ac.uk/pdbsum/7o54 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7o54 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 07:34, 5 May 2021

2021-05-05T07:34:42Z

←Older revision		Revision as of 07:34, 5 May 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7o54 is ON HOLD	+	The entry 7o54 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7o54" [edit=sysop:move=sysop]

2021-04-14T05:51:03Z

Protected "7o54" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:51, 14 April 2021

OCA: New page: '''Unreleased structure''' The entry 7o54 is ON HOLD Authors: Description: Category: Unreleased Structures

2021-04-14T05:51:02Z

New page: '''Unreleased structure''' The entry 7o54 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7o54 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7o54 - Revision history

OCA at 12:44, 1 February 2024

OCA at 06:16, 10 November 2021

OCA at 07:34, 5 May 2021

OCA: Protected "7o54" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7o54 is ON HOLD Authors: Description: Category: Unreleased Structures