7o9p - Revision history

OCA at 07:13, 2 February 2022

2022-02-02T07:13:04Z

←Older revision		Revision as of 07:13, 2 February 2022
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	==Crystal structure of the Awp3b (adhesin-like wall protein 3b) A-domain from Candida glabrata showing a gadolinium cluster==		==Crystal structure of the Awp3b (adhesin-like wall protein 3b) A-domain from Candida glabrata showing a gadolinium cluster==
-	<StructureSection load='7o9p' size='340' side='right'caption='[[7o9p]]' scene=''>	+	<StructureSection load='7o9p' size='340' side='right'caption='[[7o9p]], [[Resolution\|resolution]] 1.99Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7O9P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7O9P FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7o9p]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7O9P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7O9P FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7o9p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7o9p OCA], [https://pdbe.org/7o9p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7o9p RCSB], [https://www.ebi.ac.uk/pdbsum/7o9p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7o9p ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GD:GADOLINIUM+ATOM'>GD</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7o9p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7o9p OCA], [https://pdbe.org/7o9p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7o9p RCSB], [https://www.ebi.ac.uk/pdbsum/7o9p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7o9p ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Candida glabrata is an opportunistic pathogenic yeast frequently causing infections in humans. Though it lacks typical virulence factors such as hyphal development, C. glabrata contains a remarkably large and diverse set of putative wall adhesins that is crucial for its success as pathogen. Here, we present an analysis of putative adhesins from the homology clusters V and VI. First, sequence similarity network analysis revealed relationships between cluster V and VI adhesins and S. cerevisiae haze protective factors (Hpf). Crystal structures of A-regions from cluster VI adhesins Awp1 and Awp3b reveal a parallel right-handed beta-helix domain that is linked to a C-terminal beta-sandwich. Structure solution of the A-region of Awp3b via single wavelength anomalous diffraction phasing revealed the largest known lanthanide cluster with 21 Gd3+ ions. Awp1-A and Awp3b-A show structural similarity to pectate lyases but binding to neither carbohydrates nor Ca2+ was observed. Phenotypic analysis of awp1Delta, awp3Delta, and awp1,3Delta double mutants did also not confirm their role as adhesins. In contrast, deletion mutants of the cluster V adhesin Awp2 in the hyperadhesive clinical isolate PEU382 demonstrated its importance for adhesion to polystyrene or glass, biofilm formation, cell aggregation and other cell surface-related phenotypes. Together with cluster III and VII adhesins our study shows that C. glabrata CBS138 can rely on a set of 42 Awp1-related adhesins with beta-helix/alpha-crystallin domain architecture for modifying the surface characteristics of its cell wall.
		+
		+	A novel class of Candida glabrata cell wall proteins with beta-helix fold mediates adhesion in clinical isolates.,Reithofer V, Fernandez-Pereira J, Alvarado M, de Groot P, Essen LO PLoS Pathog. 2021 Dec 28;17(12):e1009980. doi: 10.1371/journal.ppat.1009980., eCollection 2021 Dec. PMID:34962966<ref>PMID:34962966</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7o9p" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Essen L-O]]	+	[[Category: Essen, L O]]
-	[[Category: Reithofer V]]	+	[[Category: Groot, P de]]
-	[[Category: ~~De Groot P~~]]	+	[[Category: Reithofer, V]]
		+	[[Category: Adhesin]]
		+	[[Category: Adhesin-like wall protein]]
		+	[[Category: Adhesion]]
		+	[[Category: Awp]]
		+	[[Category: Beta-helix]]
		+	[[Category: Candida glabrata]]
		+	[[Category: Cell adhesion]]
		+	[[Category: Gadolinium]]
		+	[[Category: Gadolinium cluster]]
		+	[[Category: Haze-protective factor]]
		+	[[Category: Lanthanide]]
		+	[[Category: Lanthanide cluster]]

OCA at 16:48, 15 December 2021

2021-12-15T16:48:29Z

←Older revision		Revision as of 16:48, 15 December 2021
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-	'''Unreleased structure'''

-	~~The entry 7o9p is ON HOLD until Paper Publication~~	+	==Crystal structure of the Awp3b (adhesin-like wall protein 3b) A-domain from Candida glabrata showing a gadolinium cluster==
-		+	<StructureSection load='7o9p' size='340' side='right'caption='[[7o9p]]' scene=''>
-	~~Authors: Reithofer, V., de Groot, P., Essen, L.-O.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7O9P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7O9P FirstGlance]. <br>
-	~~Description:~~ Crystal structure of the Awp3b (adhesin-like wall protein 3b) A-domain from Candida glabrata showing a gadolinium cluster	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7o9p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7o9p OCA], [https://pdbe.org/7o9p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7o9p RCSB], [https://www.ebi.ac.uk/pdbsum/7o9p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7o9p ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[~~Category~~: ~~De Groot~~, P]]	+	__TOC__
-	[[Category: ~~Reithofer, V~~]]	+	</StructureSection>
-	[[Category: Essen, L.-O]]	+	[[Category: Large Structures]]
		+	[[Category: Essen L-O]]
		+	[[Category: Reithofer V]]
		+	[[Category: De Groot P]]

OCA at 09:02, 19 May 2021

2021-05-19T09:02:49Z

←Older revision		Revision as of 09:02, 19 May 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7o9p is ON HOLD	+	The entry 7o9p is ON HOLD until Paper Publication

	Authors: Reithofer, V., de Groot, P., Essen, L.-O.		Authors: Reithofer, V., de Groot, P., Essen, L.-O.

OCA: Protected "7o9p" [edit=sysop:move=sysop]

2021-04-28T04:25:43Z

Protected "7o9p" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:25, 28 April 2021

OCA: New page: '''Unreleased structure''' The entry 7o9p is ON HOLD Authors: Reithofer, V., de Groot, P., Essen, L.-O. Description: Crystal structure of the Awp3b (adhesin-like wall protein 3b) A-dom...

2021-04-28T04:25:42Z

New page: '''Unreleased structure''' The entry 7o9p is ON HOLD Authors: Reithofer, V., de Groot, P., Essen, L.-O. Description: Crystal structure of the Awp3b (adhesin-like wall protein 3b) A-dom...

New page

'''Unreleased structure'''

The entry 7o9p is ON HOLD

Authors: Reithofer, V., de Groot, P., Essen, L.-O.

Description: Crystal structure of the Awp3b (adhesin-like wall protein 3b) A-domain from Candida glabrata showing a gadolinium cluster
[[Category: Unreleased Structures]]
[[Category: De Groot, P]]
[[Category: Reithofer, V]]
[[Category: Essen, L.-O]]