OCA at 20:22, 16 November 2022

2022-11-16T20:22:19Z

←Older revision		Revision as of 20:22, 16 November 2022
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	==Cryo-EM structure of the plectasin fibril (single strand)==		==Cryo-EM structure of the plectasin fibril (single strand)==
-	<StructureSection load='7oag' size='340' side='right'caption='[[7oag]]' scene=''>	+	<StructureSection load='7oag' size='340' side='right'caption='[[7oag]], [[Resolution\|resolution]] 3.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7OAG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7OAG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7oag]] is a 25 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudoplectania_nigrella Pseudoplectania nigrella]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7OAG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7OAG FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7oag FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7oag OCA], [https://pdbe.org/7oag PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7oag RCSB], [https://www.ebi.ac.uk/pdbsum/7oag PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7oag ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7oag FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7oag OCA], [https://pdbe.org/7oag PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7oag RCSB], [https://www.ebi.ac.uk/pdbsum/7oag PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7oag ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/DEFPL_PSENR DEFPL_PSENR] Antimicrobial peptide that potently acts against several species of Gram-positive bacteria (PubMed:16222292, PubMed:19472324). It selectively inhibits peptidoglycan biosynthesis through complex formation with the cell wall precursor lipid II (1:1 molar ratio) thus inhibiting cell wall synthesis (PubMed:20508130). It does not disrupt cell membranes (PubMed:20508130). Is especially active against numerous clinical isolates of S.pneumoniae, including all 90 different serotypes and isolates resistant to clinically used antibiotics (PubMed:16222292). In vitro, shows considerable selectivity for bacteria over mammalian cells (PubMed:16222292). The peptide synthesized in D-amino acids does not show antibacterial activity (PubMed:19472324). In vitro, acts on voltage-gated potassium channels by moderately inhibiting mammalian Kv1.3/KCNA3 (IC(50)=2.8 uM), and moderately inhibiting others potassium channels (PubMed:25568977).<ref>PMID:16222292</ref> <ref>PMID:25568977</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Self-assembly and fibril formation play important roles in protein behaviour. Amyloid fibril formation is well-studied due to its role in neurodegenerative diseases and characterized by refolding of the protein into predominantly beta-sheet form. However, much less is known about the assembly of proteins into other types of supramolecular structures. Using cryo-electron microscopy at a resolution of 1.97 A, we show that a triple-mutant of the anti-microbial peptide plectasin, PPI42, assembles into helical non-amyloid fibrils. The in vitro anti-microbial activity was determined and shown to be enhanced compared to the wildtype. Plectasin contains a cysteine-stabilised alpha-helix-beta-sheet structure, which remains intact upon fibril formation. Two protofilaments form a right-handed protein fibril. The fibril formation is reversible and follows sigmoidal kinetics with a pH- and concentration dependent equilibrium between soluble monomer and protein fibril. This high-resolution structure reveals that alpha/beta proteins can natively assemble into fibrils.
		+
		+	pH- and concentration-dependent supramolecular assembly of a fungal defensin plectasin variant into helical non-amyloid fibrils.,Pohl C, Effantin G, Kandiah E, Meier S, Zeng G, Streicher W, Segura DR, Mygind PH, Sandvang D, Nielsen LA, Peters GHJ, Schoehn G, Mueller-Dieckmann C, Noergaard A, Harris P Nat Commun. 2022 Jun 7;13(1):3162. doi: 10.1038/s41467-022-30462-w. PMID:35672293<ref>PMID:35672293</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7oag" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Pseudoplectania nigrella]]
	[[Category: Effantin G]]		[[Category: Effantin G]]

OCA at 11:38, 27 April 2022

2022-04-27T11:38:08Z

←Older revision		Revision as of 11:38, 27 April 2022
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-	'''Unreleased structure'''

-	~~The entry 7oag is ON HOLD until Paper Publication~~	+	==Cryo-EM structure of the plectasin fibril (single strand)==
-		+	<StructureSection load='7oag' size='340' side='right'caption='[[7oag]]' scene=''>
-	~~Authors: Effantin, G.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7OAG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7OAG FirstGlance]. <br>
-	~~Description:~~ Cryo-EM structure of the plectasin fibril (single strand)	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7oag FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7oag OCA], [https://pdbe.org/7oag PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7oag RCSB], [https://www.ebi.ac.uk/pdbsum/7oag PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7oag ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: Effantin, G]]	+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Effantin G]]

OCA at 09:03, 19 May 2021

2021-05-19T09:03:27Z

←Older revision		Revision as of 09:03, 19 May 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7oag is ON HOLD	+	The entry 7oag is ON HOLD until Paper Publication

	Authors: Effantin, G.		Authors: Effantin, G.

OCA: Protected "7oag" [edit=sysop:move=sysop]

2021-04-28T04:27:03Z

Protected "7oag" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:27, 28 April 2021

OCA: New page: '''Unreleased structure''' The entry 7oag is ON HOLD Authors: Effantin, G. Description: Cryo-EM structure of the plectasin fibril (single strand) Category: Unreleased Structures [[...

2021-04-28T04:27:02Z

New page: '''Unreleased structure''' The entry 7oag is ON HOLD Authors: Effantin, G. Description: Cryo-EM structure of the plectasin fibril (single strand) Category: Unreleased Structures [[...

New page

'''Unreleased structure'''

The entry 7oag is ON HOLD

Authors: Effantin, G.

Description: Cryo-EM structure of the plectasin fibril (single strand)
[[Category: Unreleased Structures]]
[[Category: Effantin, G]]

7oag - Revision history

OCA at 20:22, 16 November 2022

OCA at 11:38, 27 April 2022

OCA at 09:03, 19 May 2021

OCA: Protected "7oag" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7oag is ON HOLD Authors: Effantin, G. Description: Cryo-EM structure of the plectasin fibril (single strand) Category: Unreleased Structures [[...