OCA at 12:49, 1 February 2024

2024-02-01T12:49:24Z

←Older revision		Revision as of 12:49, 1 February 2024
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-	====	+	==Crystal structure of TD1-mebendazole complex==
-	<StructureSection load='7odn' size='340' side='right'caption='[[7odn]]' scene=''>	+	<StructureSection load='7odn' size='340' side='right'caption='[[7odn]], [[Resolution\|resolution]] 2.33Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7odn]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ODN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ODN FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7odn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7odn OCA], [https://pdbe.org/7odn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7odn RCSB], [https://www.ebi.ac.uk/pdbsum/7odn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7odn ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.33Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=V95:methyl+N-(6-benzoyl-1H-benzimidazol-2-yl)carbamate'>V95</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7odn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7odn OCA], [https://pdbe.org/7odn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7odn RCSB], [https://www.ebi.ac.uk/pdbsum/7odn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7odn ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/TBA1B_BOVIN TBA1B_BOVIN] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Microtubule targeting agents (MTAs) have been exploited mainly as anti-cancer drugs because of their impact on cellular division and angiogenesis. Additionally, microtubules (MTs) are key structures for intracellular transport, which is frequently hijacked during viral infection. We have analyzed the antiviral activity of clinically used MTAs in the infection of DNA and RNA viruses, including SARS-CoV-2, to find that MT destabilizer agents show a higher impact than stabilizers in the viral infections tested, and FDA-approved anti-helminthic benzimidazoles were among the most active compounds. In order to understand the reasons for the observed antiviral activity, we studied the impact of these compounds in motor proteins-mediated intracellular transport. To do so, we used labeled peptide tools, finding that clinically available MTAs impaired the movement linked to MT motors in living cells. However, their effect on viral infection lacked a clear correlation to their effect in motor-mediated transport, denoting the complex use of the cytoskeleton by viruses. Finally, we further delved into the molecular mechanism of action of Mebendazole by combining biochemical and structural studies to obtain crystallographic high-resolution information of the Mebendazole-tubulin complex, which provided insights into the mechanisms of differential toxicity between helminths and mammalians.
		+
		+	Effect of Clinically Used Microtubule Targeting Drugs on Viral Infection and Transport Function.,Oliva MA, Tosat-Bitrian C, Barrado-Gil L, Bonato F, Galindo I, Garaigorta U, Alvarez-Bernad B, Paris-Ogayar R, Lucena-Agell D, Gimenez-Abian JF, Garcia-Dorival I, Urquiza J, Gastaminza P, Diaz JF, Palomo V, Alonso C Int J Mol Sci. 2022 Mar 22;23(7):3448. doi: 10.3390/ijms23073448. PMID:35408808<ref>PMID:35408808</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7odn" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Tubulin 3D Structures\|Tubulin 3D Structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bos taurus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Synthetic construct]]
		+	[[Category: Bonato F]]
		+	[[Category: Diaz JF]]
		+	[[Category: Oliva MA]]

OCA at 10:23, 6 April 2022

2022-04-06T10:23:47Z

←Older revision		Revision as of 10:23, 6 April 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7odn ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7odn' size='340' side='right'caption='[[7odn]]' scene=''>
-	~~Authors~~: ~~Oliva, M~~.A.~~, Bonato, F~~.~~, Diaz, J~~.F.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~: ~~Crystal structure of TD1-mebendazole complex~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7odn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7odn OCA], [https://pdbe.org/7odn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7odn RCSB], [https://www.ebi.ac.uk/pdbsum/7odn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7odn ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[~~Category~~: ~~Oliva~~, M.A]]	+	__TOC__
-	[[Category: ~~Bonato, F~~]]	+	</StructureSection>
-	[[Category: ~~Diaz, J.F~~]]	+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 09:04, 19 May 2021

2021-05-19T09:04:18Z

←Older revision		Revision as of 09:04, 19 May 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7odn is ON HOLD	+	The entry 7odn is ON HOLD until Paper Publication

	Authors: Oliva, M.A., Bonato, F., Diaz, J.F.		Authors: Oliva, M.A., Bonato, F., Diaz, J.F.

OCA: Protected "7odn" [edit=sysop:move=sysop]

2021-05-12T08:22:14Z

Protected "7odn" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:22, 12 May 2021

OCA: New page: '''Unreleased structure''' The entry 7odn is ON HOLD Authors: Oliva, M.A., Bonato, F., Diaz, J.F. Description: Crystal structure of TD1-mebendazole complex [[Category: Unreleased Struc...

2021-05-12T08:22:13Z

New page: '''Unreleased structure''' The entry 7odn is ON HOLD Authors: Oliva, M.A., Bonato, F., Diaz, J.F. Description: Crystal structure of TD1-mebendazole complex [[Category: Unreleased Struc...

New page

'''Unreleased structure'''

The entry 7odn is ON HOLD

Authors: Oliva, M.A., Bonato, F., Diaz, J.F.

Description: Crystal structure of TD1-mebendazole complex
[[Category: Unreleased Structures]]
[[Category: Oliva, M.A]]
[[Category: Bonato, F]]
[[Category: Diaz, J.F]]

7odn - Revision history

OCA at 12:49, 1 February 2024

OCA at 10:23, 6 April 2022

OCA at 09:04, 19 May 2021

OCA: Protected "7odn" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7odn is ON HOLD Authors: Oliva, M.A., Bonato, F., Diaz, J.F. Description: Crystal structure of TD1-mebendazole complex [[Category: Unreleased Struc...