OCA at 12:51, 1 February 2024

2024-02-01T12:51:22Z

←Older revision		Revision as of 12:51, 1 February 2024
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-	====	+	==E2 UBE2K covalently linked to donor Ub, acceptor di-Ub, and RING E3 primed for K48-linked Ub chain synthesis==
-	<StructureSection load='7ojx' size='340' side='right'caption='[[7ojx]]' scene=''>	+	<StructureSection load='7ojx' size='340' side='right'caption='[[7ojx]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7ojx]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7OJX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7OJX FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ojx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ojx OCA], [https://pdbe.org/7ojx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ojx RCSB], [https://www.ebi.ac.uk/pdbsum/7ojx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ojx ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.4Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ME7:1,1-ETHANE-1,2-DIYLBIS(1H-PYRROLE-2,5-DIONE)'>ME7</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ojx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ojx OCA], [https://pdbe.org/7ojx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ojx RCSB], [https://www.ebi.ac.uk/pdbsum/7ojx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ojx ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/RNF38_HUMAN RNF38_HUMAN]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Ubiquitin (Ub) chain types govern distinct biological processes. K48-linked polyUb chains target substrates for proteasomal degradation, but the mechanism of Ub chain synthesis remains elusive due to the transient nature of Ub handover. Here, we present the structure of a chemically trapped complex of the E2 UBE2K covalently linked to donor Ub and acceptor K48-linked di-Ub, primed for K48-linked Ub chain synthesis by a RING E3. The structure reveals the basis for acceptor Ub recognition by UBE2K active site residues and the C-terminal Ub-associated (UBA) domain, to impart K48-linked Ub specificity and catalysis. Furthermore, the structure unveils multiple Ub-binding surfaces on the UBA domain that allow distinct binding modes for K48- and K63-linked Ub chains. This multivalent Ub-binding feature serves to recruit UBE2K to ubiquitinated substrates to overcome weak acceptor Ub affinity and thereby promote chain elongation. These findings elucidate the mechanism of processive K48-linked polyUb chain formation by UBE2K.
		+
		+	Structure of UBE2K-Ub/E3/polyUb reveals mechanisms of K48-linked Ub chain extension.,Nakasone MA, Majorek KA, Gabrielsen M, Sibbet GJ, Smith BO, Huang DT Nat Chem Biol. 2022 Apr;18(4):422-431. doi: 10.1038/s41589-021-00952-x. Epub 2022 , Jan 13. PMID:35027744<ref>PMID:35027744</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7ojx" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Ubiquitin protein ligase 3D structures\|Ubiquitin protein ligase 3D structures]]
		+	*[[3D structures of ubiquitin\|3D structures of ubiquitin]]
		+	*[[3D structures of ubiquitin conjugating enzyme\|3D structures of ubiquitin conjugating enzyme]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Huang DT]]
		+	[[Category: Majorek KA]]
		+	[[Category: Nakasone MA]]

OCA at 09:57, 12 January 2022

2022-01-12T09:57:51Z

←Older revision		Revision as of 09:57, 12 January 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7ojx ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7ojx' size='340' side='right'caption='[[7ojx]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ojx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ojx OCA], [https://pdbe.org/7ojx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ojx RCSB], [https://www.ebi.ac.uk/pdbsum/7ojx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ojx ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 13:36, 2 June 2021

2021-06-02T13:36:30Z

←Older revision		Revision as of 13:36, 2 June 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7ojx is ON HOLD	+	The entry 7ojx is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7ojx" [edit=sysop:move=sysop]

2021-05-26T08:08:39Z

Protected "7ojx" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:08, 26 May 2021

OCA: New page: '''Unreleased structure''' The entry 7ojx is ON HOLD Authors: Description: Category: Unreleased Structures

2021-05-26T08:08:38Z

New page: '''Unreleased structure''' The entry 7ojx is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7ojx is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7ojx - Revision history

OCA at 12:51, 1 February 2024

OCA at 09:57, 12 January 2022

OCA at 13:36, 2 June 2021

OCA: Protected "7ojx" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7ojx is ON HOLD Authors: Description: Category: Unreleased Structures