7ono - Revision history

OCA at 12:53, 1 February 2024

2024-02-01T12:53:20Z

←Older revision		Revision as of 12:53, 1 February 2024
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	<StructureSection load='7ono' size='340' side='right'caption='[[7ono]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='7ono' size='340' side='right'caption='[[7ono]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7ono]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Ecoli Ecoli~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ONO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ONO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7ono]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ONO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ONO FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=TMO:TRIMETHYLAMINE+OXIDE'>TMO</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.3Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>~~[[~~Related_structure~~\|~~Related:~~]]~~</b></td><td class="sblockDat"><div style='overflow: auto~~; ~~max-height: 3em;'>[[7onn\|7onn]]</div>~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=TMO:TRIMETHYLAMINE+OXIDE'>TMO</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>ftsI, pbpB, b0084, JW0082 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~83333 ECOLI])</td~~><~~/tr>~~	+
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>[https://en.wikipedia.org/wiki/Serine-type_D-Ala-D-Ala_carboxypeptidase Serine-type D-Ala-D-Ala carboxypeptidase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.16.4 3.4.16.4] </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ono FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ono OCA], [https://pdbe.org/7ono PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ono RCSB], [https://www.ebi.ac.uk/pdbsum/7ono PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ono ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ono FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ono OCA], [https://pdbe.org/7ono PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ono RCSB], [https://www.ebi.ac.uk/pdbsum/7ono PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ono ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/FTSI_ECOLI FTSI_ECOLI]] Essential cell division protein that catalyzes cross-linking of the peptidoglycan cell wall at the division septum (PubMed:1103132, PubMed:6450748, PubMed:9614966, PubMed:3531167, PubMed:7030331). Required for localization of FtsN (PubMed:9282742).<ref>PMID:1103132</ref> <ref>PMID:3531167</ref> <ref>PMID:6450748</ref> <ref>PMID:7030331</ref> <ref>PMID:9282742</ref> <ref>PMID:9614966</ref>	+	[https://www.uniprot.org/uniprot/FTSI_ECOLI FTSI_ECOLI] Essential cell division protein that catalyzes cross-linking of the peptidoglycan cell wall at the division septum (PubMed:1103132, PubMed:6450748, PubMed:9614966, PubMed:3531167, PubMed:7030331). Required for localization of FtsN (PubMed:9282742).<ref>PMID:1103132</ref> <ref>PMID:3531167</ref> <ref>PMID:6450748</ref> <ref>PMID:7030331</ref> <ref>PMID:9282742</ref> <ref>PMID:9614966</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 7ono" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 7ono" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Penicillin-binding protein 3D structures\|Penicillin-binding protein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Ecoli~~]]	+	[[Category: Escherichia coli K-12]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Serine-type D-Ala-D-Ala carboxypeptidase]]~~	+	[[Category: Freischem S]]
-	[[Category: Freischem, S]]	+	[[Category: Grimm I]]
-	[[Category: Grimm, I]]	+	[[Category: Weiergraeber OH]]
-	[[Category: Weiergraeber~~, O H]]~~	+
-	~~[[Category: Membrane protein]]~~	+
-	~~[[Category: Pbp3]]~~	+
-	~~[[Category: Peptidoglycan synthesis]]~~	+
-	~~[[Category: Transpeptidase domain~~]]	+

OCA at 07:04, 22 September 2021

2021-09-22T07:04:24Z

←Older revision		Revision as of 07:04, 22 September 2021
Line 1:		Line 1:

	==Crystal structure of PBP3 transpeptidase domain from E. coli==		==Crystal structure of PBP3 transpeptidase domain from E. coli==
-	<StructureSection load='7ono' size='340' side='right'caption='[[7ono]]' scene=''>	+	<StructureSection load='7ono' size='340' side='right'caption='[[7ono]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ONO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ONO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7ono]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ONO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ONO FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ono FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ono OCA], [https://pdbe.org/7ono PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ono RCSB], [https://www.ebi.ac.uk/pdbsum/7ono PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ono ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=TMO:TRIMETHYLAMINE+OXIDE'>TMO</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[7onn\|7onn]]</div></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ftsI, pbpB, b0084, JW0082 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Serine-type_D-Ala-D-Ala_carboxypeptidase Serine-type D-Ala-D-Ala carboxypeptidase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.16.4 3.4.16.4] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ono FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ono OCA], [https://pdbe.org/7ono PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ono RCSB], [https://www.ebi.ac.uk/pdbsum/7ono PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ono ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/FTSI_ECOLI FTSI_ECOLI]] Essential cell division protein that catalyzes cross-linking of the peptidoglycan cell wall at the division septum (PubMed:1103132, PubMed:6450748, PubMed:9614966, PubMed:3531167, PubMed:7030331). Required for localization of FtsN (PubMed:9282742).<ref>PMID:1103132</ref> <ref>PMID:3531167</ref> <ref>PMID:6450748</ref> <ref>PMID:7030331</ref> <ref>PMID:9282742</ref> <ref>PMID:9614966</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Novel antimicrobial strategies are urgently required because of the rising threat of multi drug resistant bacterial strains and the infections caused by them. Among the available target structures, the so-called penicillin binding proteins are of particular interest, owing to their good accessibility in the periplasmic space, and the lack of homologous proteins in humans, reducing the risk of side effects of potential drugs. In this report, we focus on the interaction of the innovative beta-lactam antibiotic AIC499 with penicillin binding protein 3 (PBP3) from Escherichia coli and Pseudomonas aeruginosa. This recently developed monobactam displays broad antimicrobial activity, against Gram-negative strains, and improved resistance to most classes of beta-lactamases. By analyzing crystal structures of the respective complexes, we were able to explore the binding mode of AIC499 to its target proteins. In addition, the apo structures determined for PBP3, from P. aeruginosa and the catalytic transpeptidase domain of the E. coli orthologue, provide new insights into the dynamics of these proteins and the impact of drug binding.
		+
		+	Interaction Mode of the Novel Monobactam AIC499 Targeting Penicillin Binding Protein 3 of Gram-Negative Bacteria.,Freischem S, Grimm I, Lopez-Perez A, Willbold D, Klenke B, Vuong C, Dingley AJ, Weiergraber OH Biomolecules. 2021 Jul 19;11(7). pii: biom11071057. doi: 10.3390/biom11071057. PMID:34356681<ref>PMID:34356681</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7ono" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Ecoli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Freischem S]]	+	[[Category: Serine-type D-Ala-D-Ala carboxypeptidase]]
-	[[Category: Grimm I]]	+	[[Category: Freischem, S]]
-	[[Category: Weiergraeber OH]]	+	[[Category: Grimm, I]]
		+	[[Category: Weiergraeber, O H]]
		+	[[Category: Membrane protein]]
		+	[[Category: Pbp3]]
		+	[[Category: Peptidoglycan synthesis]]
		+	[[Category: Transpeptidase domain]]

OCA at 10:21, 4 August 2021

2021-08-04T10:21:04Z

←Older revision		Revision as of 10:21, 4 August 2021
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7ono ~~is ON HOLD until Paper Publication~~	+	==Crystal structure of PBP3 transpeptidase domain from E. coli==
-		+	<StructureSection load='7ono' size='340' side='right'caption='[[7ono]]' scene=''>
-	~~Authors~~: ~~Freischem~~, S., ~~Grimm~~, I., ~~Weiergraeber~~, O.H.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ONO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ONO FirstGlance]. <br>
-	~~Description: Crystal structure of PBP3 transpeptidase domain from E. coli~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ono FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ono OCA], [https://pdbe.org/7ono PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ono RCSB], [https://www.ebi.ac.uk/pdbsum/7ono PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ono ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: Freischem, S]]	+	__TOC__
-	[[Category: Grimm, I]]	+	</StructureSection>
-	[[Category: Weiergraeber~~, O.H~~]]	+	[[Category: Large Structures]]
		+	[[Category: Freischem S]]
		+	[[Category: Grimm I]]
		+	[[Category: Weiergraeber OH]]

OCA at 11:12, 9 June 2021

2021-06-09T11:12:50Z

←Older revision		Revision as of 11:12, 9 June 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7ono is ON HOLD	+	The entry 7ono is ON HOLD until Paper Publication

	Authors: Freischem, S., Grimm, I., Weiergraeber, O.H.		Authors: Freischem, S., Grimm, I., Weiergraeber, O.H.

OCA: Protected "7ono" [edit=sysop:move=sysop]

2021-06-02T13:40:15Z

Protected "7ono" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:40, 2 June 2021

OCA: New page: '''Unreleased structure''' The entry 7ono is ON HOLD Authors: Freischem, S., Grimm, I., Weiergraeber, O.H. Description: Crystal structure of PBP3 transpeptidase domain from E. coli [[C...

2021-06-02T13:40:14Z

New page: '''Unreleased structure''' The entry 7ono is ON HOLD Authors: Freischem, S., Grimm, I., Weiergraeber, O.H. Description: Crystal structure of PBP3 transpeptidase domain from E. coli [[C...

New page

'''Unreleased structure'''

The entry 7ono is ON HOLD

Authors: Freischem, S., Grimm, I., Weiergraeber, O.H.

Description: Crystal structure of PBP3 transpeptidase domain from E. coli
[[Category: Unreleased Structures]]
[[Category: Freischem, S]]
[[Category: Grimm, I]]
[[Category: Weiergraeber, O.H]]