OCA at 09:05, 29 September 2021

2021-09-29T09:05:58Z

←Older revision		Revision as of 09:05, 29 September 2021
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	==Cryo-EM structure of ABCG1 E242Q mutant with ATP and cholesteryl hemisuccinate bound==		==Cryo-EM structure of ABCG1 E242Q mutant with ATP and cholesteryl hemisuccinate bound==
-	<StructureSection load='7oz1' size='340' side='right'caption='[[7oz1]]' scene=''>	+	<StructureSection load='7oz1' size='340' side='right'caption='[[7oz1]], [[Resolution\|resolution]] 4.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7OZ1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7OZ1 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7oz1]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7OZ1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7OZ1 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7oz1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7oz1 OCA], [https://pdbe.org/7oz1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7oz1 RCSB], [https://www.ebi.ac.uk/pdbsum/7oz1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7oz1 ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=PEE:1,2-DIOLEOYL-SN-GLYCERO-3-PHOSPHOETHANOLAMINE'>PEE</scene>, <scene name='pdbligand=Y01:CHOLESTEROL+HEMISUCCINATE'>Y01</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7oz1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7oz1 OCA], [https://pdbe.org/7oz1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7oz1 RCSB], [https://www.ebi.ac.uk/pdbsum/7oz1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7oz1 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/ABCG1_HUMAN ABCG1_HUMAN]] Catalyzes the efflux of phospholipids such as sphingomyelin, cholesterol and its oxygenated derivatives like 7beta-hydroxycholesterol and this transport is coupled to hydrolysis of ATP (PubMed:17408620, PubMed:24576892). The lipid efflux is ALB-dependent (PubMed:16702602). Is an active component of the macrophage lipid export complex. Could also be involved in intracellular lipid transport processes. The role in cellular lipid homeostasis may not be limited to macrophages. Prevents cell death by transporting cytotoxic 7beta-hydroxycholesterol (PubMed:17408620).<ref>PMID:16702602</ref> <ref>PMID:17408620</ref> <ref>PMID:24576892</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	ABCG1 is an ATP binding cassette (ABC) transporter that removes excess cholesterol from peripheral tissues. Despite its role in preventing lipid accumulation and the development of cardiovascular and metabolic disease, the mechanism underpinning ABCG1-mediated cholesterol transport is unknown. Here we report a cryo-EM structure of human ABCG1 at 4 A resolution in an inward-open state, featuring sterol-like density in the binding cavity. Structural comparison with the multidrug transporter ABCG2 and the sterol transporter ABCG5/G8 reveals the basis of mechanistic differences and distinct substrate specificity. Benzamil and taurocholate inhibited the ATPase activity of liposome-reconstituted ABCG1, whereas the ABCG2 inhibitor Ko143 did not. Based on the structural insights into ABCG1, we propose a mechanism for ABCG1-mediated cholesterol transport.
		+
		+	Structure of the Human Cholesterol Transporter ABCG1.,Skarda L, Kowal J, Locher KP J Mol Biol. 2021 Aug 28;433(21):167218. doi: 10.1016/j.jmb.2021.167218. PMID:34461069<ref>PMID:34461069</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7oz1" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Kowal J]]	+	[[Category: Kowal, J]]
-	[[Category: Locher KP]]	+	[[Category: Locher, K P]]
-	[[Category: Skarda L]]	+	[[Category: Skarda, L]]
		+	[[Category: Abc transporter]]
		+	[[Category: Atp binding]]
		+	[[Category: Cholesterol]]
		+	[[Category: Inward-open]]
		+	[[Category: Membrane protein]]

OCA at 06:52, 22 September 2021

2021-09-22T06:52:47Z

←Older revision		Revision as of 06:52, 22 September 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7oz1 is ~~ON HOLD until Paper Publication~~	+	==Cryo-EM structure of ABCG1 E242Q mutant with ATP and cholesteryl hemisuccinate bound==
-		+	<StructureSection load='7oz1' size='340' side='right'caption='[[7oz1]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7OZ1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7OZ1 FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7oz1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7oz1 OCA], [https://pdbe.org/7oz1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7oz1 RCSB], [https://www.ebi.ac.uk/pdbsum/7oz1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7oz1 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Kowal J]]
		+	[[Category: Locher KP]]
		+	[[Category: Skarda L]]

OCA at 08:49, 14 July 2021

2021-07-14T08:49:37Z

←Older revision		Revision as of 08:49, 14 July 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7oz1 is ON HOLD	+	The entry 7oz1 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7oz1" [edit=sysop:move=sysop]

2021-07-07T08:52:12Z

Protected "7oz1" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:52, 7 July 2021

OCA: New page: '''Unreleased structure''' The entry 7oz1 is ON HOLD Authors: Description: Category: Unreleased Structures

2021-07-07T08:52:11Z

New page: '''Unreleased structure''' The entry 7oz1 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7oz1 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7oz1 - Revision history

OCA at 09:05, 29 September 2021

OCA at 06:52, 22 September 2021

OCA at 08:49, 14 July 2021

OCA: Protected "7oz1" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7oz1 is ON HOLD Authors: Description: Category: Unreleased Structures