OCA at 13:02, 1 February 2024

2024-02-01T13:02:23Z

←Older revision		Revision as of 13:02, 1 February 2024
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-	====	+	==Crystal structure of in planta processed AvrRps4 in complex with the WRKY domain of RRS1==
-	<StructureSection load='7p8k' size='340' side='right'caption='[[7p8k]]' scene=''>	+	<StructureSection load='7p8k' size='340' side='right'caption='[[7p8k]], [[Resolution\|resolution]] 2.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7p8k]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana] and [https://en.wikipedia.org/wiki/Pseudomonas_syringae Pseudomonas syringae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7P8K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7P8K FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7p8k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7p8k OCA], [https://pdbe.org/7p8k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7p8k RCSB], [https://www.ebi.ac.uk/pdbsum/7p8k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7p8k ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.65Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7p8k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7p8k OCA], [https://pdbe.org/7p8k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7p8k RCSB], [https://www.ebi.ac.uk/pdbsum/7p8k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7p8k ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/WR52W_ARATH WR52W_ARATH] Transcription factor. Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element. Acts also as a disease resistance protein involved in resistance to fungal and bacterial pathogens, including R.solanacearum, P.syringae pv. tomato and C.higginsianum. In presence of RPS4, elicites an EDS1-dependent hypersensitive response (PubMed:24146667).[UniProtKB:Q9FH83]<ref>PMID:19519800</ref> <ref>PMID:19826224</ref> <ref>PMID:24146667</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Plants use intracellular nucleotide-binding domain (NBD) and leucine-rich repeat (LRR)-containing immune receptors (NLRs) to detect pathogen-derived effector proteins. The Arabidopsis NLR pair RRS1-R/RPS4 confers disease resistance to different bacterial pathogens by perceiving the structurally distinct effectors AvrRps4 from Pseudomonas syringae pv. pisi and PopP2 from Ralstonia solanacearum via an integrated WRKY domain in RRS1-R. How the WRKY domain of RRS1 (RRS1(WRKY)) perceives distinct classes of effector to initiate an immune response is unknown. Here, we report the crystal structure of the in planta processed C-terminal domain of AvrRps4 (AvrRps4(C)) in complex with RRS1(WRKY) Perception of AvrRps4(C) by RRS1(WRKY) is mediated by the beta2-beta3 segment of RRS1(WRKY) that binds an electronegative patch on the surface of AvrRps4(C) Structure-based mutations that disrupt AvrRps4(C)-RRS1(WRKY) interactions in vitro compromise RRS1/RPS4-dependent immune responses. We also show that AvrRps4(C) can associate with the WRKY domain of the related but distinct RRS1B/RPS4B NLR pair, and the DNA-binding domain of AtWRKY41, with similar binding affinities and how effector binding interferes with WRKY-W-box DNA interactions. This work demonstrates how integrated domains in plant NLRs can directly bind structurally distinct effectors to initiate immunity.
		+
		+	Perception of structurally distinct effectors by the integrated WRKY domain of a plant immune receptor.,Mukhi N, Brown H, Gorenkin D, Ding P, Bentham AR, Stevenson CEM, Jones JDG, Banfield MJ Proc Natl Acad Sci U S A. 2021 Dec 14;118(50):e2113996118. doi: , 10.1073/pnas.2113996118. PMID:34880132<ref>PMID:34880132</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7p8k" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Avirulence protein 3D structures\|Avirulence protein 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Arabidopsis thaliana]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Pseudomonas syringae]]
		+	[[Category: Banfield MJ]]
		+	[[Category: Bentham AR]]
		+	[[Category: Brown H]]
		+	[[Category: Ding P]]
		+	[[Category: Gorenkin D]]
		+	[[Category: Jones JDG]]
		+	[[Category: Mukhi N]]

OCA at 10:21, 4 August 2021

2021-08-04T10:21:27Z

←Older revision		Revision as of 10:21, 4 August 2021
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7p8k ~~is ON HOLD~~	+	====
-		+	<StructureSection load='7p8k' size='340' side='right'caption='[[7p8k]]' scene=''>
-	~~Authors~~: ~~Mukhi, N~~.~~, Brown, H~~.~~, Gorenkin, D~~.~~, Ding, P~~.~~, Bentham, A~~.R.~~, Jones, J.D.G., Banfield, M.J~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~: ~~Crystal structure of in planta processed AvrRps4 in complex with the WRKY domain of RRS1~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7p8k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7p8k OCA], [https://pdbe.org/7p8k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7p8k RCSB], [https://www.ebi.ac.uk/pdbsum/7p8k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7p8k ProSAT]</span></td></tr>
-	[~~[Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[~~[Category~~: ~~Jones, J~~.D.G]]	+	__TOC__
-	[~~[Category~~: ~~Mukhi, N~~]]	+	</StructureSection>
-	[[~~Category~~: ~~Ding~~, ~~P]]~~	+	[[Category: Large Structures]]
-	[~~[Category~~: ~~Gorenkin~~, ~~D]]~~	+	[[Category: Z-disk]]
-	[~~[Category~~: ~~Bentham, A~~.R]]	+
-	[[Category: ~~Brown, H~~]]	+
-	[[Category: ~~Banfield, M.J~~]]	+

OCA: Protected "7p8k" [edit=sysop:move=sysop]

2021-07-28T09:32:58Z

Protected "7p8k" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:32, 28 July 2021

OCA: New page: '''Unreleased structure''' The entry 7p8k is ON HOLD Authors: Mukhi, N., Brown, H., Gorenkin, D., Ding, P., Bentham, A.R., Jones, J.D.G., Banfield, M.J. Description: Crystal structure ...

2021-07-28T09:32:57Z

New page: '''Unreleased structure''' The entry 7p8k is ON HOLD Authors: Mukhi, N., Brown, H., Gorenkin, D., Ding, P., Bentham, A.R., Jones, J.D.G., Banfield, M.J. Description: Crystal structure ...

New page

'''Unreleased structure'''

The entry 7p8k is ON HOLD

Authors: Mukhi, N., Brown, H., Gorenkin, D., Ding, P., Bentham, A.R., Jones, J.D.G., Banfield, M.J.

Description: Crystal structure of in planta processed AvrRps4 in complex with the WRKY domain of RRS1
[[Category: Unreleased Structures]]
[[Category: Jones, J.D.G]]
[[Category: Mukhi, N]]
[[Category: Ding, P]]
[[Category: Gorenkin, D]]
[[Category: Bentham, A.R]]
[[Category: Brown, H]]
[[Category: Banfield, M.J]]

7p8k - Revision history

OCA at 13:02, 1 February 2024

OCA at 10:21, 4 August 2021

OCA: Protected "7p8k" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7p8k is ON HOLD Authors: Mukhi, N., Brown, H., Gorenkin, D., Ding, P., Bentham, A.R., Jones, J.D.G., Banfield, M.J. Description: Crystal structure ...