7p8z - Revision history

OCA at 13:02, 1 February 2024

2024-02-01T13:02:41Z

←Older revision		Revision as of 13:02, 1 February 2024
Line 3:		Line 3:
	<StructureSection load='7p8z' size='340' side='right'caption='[[7p8z]], [[Resolution\|resolution]] 1.67Å' scene=''>		<StructureSection load='7p8z' size='340' side='right'caption='[[7p8z]], [[Resolution\|resolution]] 1.67Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7p8z]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7P8Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7P8Z FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7p8z]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Gammaproteobacteria_bacterium Gammaproteobacteria bacterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7P8Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7P8Z FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BTN:BIOTIN'>BTN</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=P33:3,6,9,12,15,18-HEXAOXAICOSANE-1,20-DIOL'>P33</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.67Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BTN:BIOTIN'>BTN</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=P33:3,6,9,12,15,18-HEXAOXAICOSANE-1,20-DIOL'>P33</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7p8z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7p8z OCA], [https://pdbe.org/7p8z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7p8z RCSB], [https://www.ebi.ac.uk/pdbsum/7p8z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7p8z ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7p8z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7p8z OCA], [https://pdbe.org/7p8z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7p8z RCSB], [https://www.ebi.ac.uk/pdbsum/7p8z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7p8z ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A3A4VWA2_9GAMM A0A3A4VWA2_9GAMM]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 16:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 7p8z" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 7p8z" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Avidin 3D structures\|Avidin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Gammaproteobacteria bacterium]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Avraham, O]]	+	[[Category: Avraham O]]
-	[[Category: Livnah, O]]	+	[[Category: Livnah O]]
-	~~[[Category: Avidin]]~~	+
-	~~[[Category: Biotechnology]]~~	+
-	~~[[Category: Biotin binding protein]]~~	+
-	~~[[Category: Unknown function~~]]	+

OCA at 11:58, 23 March 2022

2022-03-23T11:58:41Z

←Older revision		Revision as of 11:58, 23 March 2022
Line 1:		Line 1:

	==Short wilavidin biotin complex==		==Short wilavidin biotin complex==
-	<StructureSection load='7p8z' size='340' side='right'caption='[[7p8z]]' scene=''>	+	<StructureSection load='7p8z' size='340' side='right'caption='[[7p8z]], [[Resolution\|resolution]] 1.67Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7P8Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7P8Z FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7p8z]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7P8Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7P8Z FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7p8z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7p8z OCA], [https://pdbe.org/7p8z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7p8z RCSB], [https://www.ebi.ac.uk/pdbsum/7p8z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7p8z ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BTN:BIOTIN'>BTN</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=P33:3,6,9,12,15,18-HEXAOXAICOSANE-1,20-DIOL'>P33</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7p8z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7p8z OCA], [https://pdbe.org/7p8z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7p8z RCSB], [https://www.ebi.ac.uk/pdbsum/7p8z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7p8z ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Nature's optimization of protein functions is a highly intricate evolutionary process. In addition to optimal tertiary folding, the intramolecular recognition among the monomers that generate higher-order quaternary arrangements is driven by stabilizing interactions that have a pivotal role for ideal activity. Homotetrameric avidin and streptavidin are regularly utilized in many applications, whereby their ultra-high affinity toward biotin is dependent on their quaternary arrangements. In recent years, a new subfamily of avidins was discovered that comprises homodimers rather than tetramers, in which the high affinity toward biotin is maintained. Intriguingly, several of the respective dimers have been shown to assemble into higher-order cylindrical hexamers or octamers that dissociate into dimers upon biotin binding. Here, we present wilavidin, a newly discovered member of the dimeric subfamily, forming hexamers in the apo form, which are uniquely maintained upon biotin binding with six high-affinity binding sites. Removal of the short C-terminal segment of wilavidin resulted in the presence of the dimer only, thus emphasizing the role of this segment in stabilizing the hexamer. Utilization of a hexavalent biotin-binding form of avidin would be beneficial for expanding the biotechnological toolbox. Additionally, this unique family of dimeric avidins and their propensity to oligomerize to hexamers or octamers can serve as a basis for protein oligomerization and intermonomeric recognition as well as cumulative interactions that determine molecular assemblies.
		+
		+	Wilavidin* - a novel member of the avidin family that forms unique biotin-binding hexamers.,Avraham O, Bayer EA, Livnah O FEBS J. 2021 Nov 2. doi: 10.1111/febs.16259. PMID:34726340<ref>PMID:34726340</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7p8z" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Avraham O]]	+	[[Category: Avraham, O]]
-	[[Category: Livnah O]]	+	[[Category: Livnah, O]]
		+	[[Category: Avidin]]
		+	[[Category: Biotechnology]]
		+	[[Category: Biotin binding protein]]
		+	[[Category: Unknown function]]

OCA at 13:38, 17 November 2021

2021-11-17T13:38:46Z

←Older revision		Revision as of 13:38, 17 November 2021
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7p8z ~~is ON HOLD~~	+	==Short wilavidin biotin complex==
-		+	<StructureSection load='7p8z' size='340' side='right'caption='[[7p8z]]' scene=''>
-	~~Authors~~: ~~Avraham~~, O., ~~Livnah~~, O.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7P8Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7P8Z FirstGlance]. <br>
-	~~Description: Short wilavidin biotin complex~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7p8z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7p8z OCA], [https://pdbe.org/7p8z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7p8z RCSB], [https://www.ebi.ac.uk/pdbsum/7p8z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7p8z ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Livnah,~~ O]]	+	__TOC__
-	[[Category: ~~Avraham,~~ O]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Avraham O]]
		+	[[Category: Livnah O]]

OCA at 05:03, 11 August 2021

2021-08-11T05:03:46Z

←Older revision		Revision as of 05:03, 11 August 2021
Line 5:		Line 5:
	Authors: Avraham, O., Livnah, O.		Authors: Avraham, O., Livnah, O.

-	Description: ~~Sh-~~wilavidin biotin complex	+	Description: Short wilavidin biotin complex
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Livnah, O]]		[[Category: Livnah, O]]
	[[Category: Avraham, O]]		[[Category: Avraham, O]]

OCA: Protected "7p8z" [edit=sysop:move=sysop]

2021-08-04T09:14:24Z

Protected "7p8z" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:14, 4 August 2021

OCA: New page: '''Unreleased structure''' The entry 7p8z is ON HOLD Authors: Avraham, O., Livnah, O. Description: Sh-wilavidin biotin complex Category: Unreleased Structures [[Category: Livnah, O...

2021-08-04T09:14:23Z

New page: '''Unreleased structure''' The entry 7p8z is ON HOLD Authors: Avraham, O., Livnah, O. Description: Sh-wilavidin biotin complex Category: Unreleased Structures [[Category: Livnah, O...

New page

'''Unreleased structure'''

The entry 7p8z is ON HOLD

Authors: Avraham, O., Livnah, O.

Description: Sh-wilavidin biotin complex
[[Category: Unreleased Structures]]
[[Category: Livnah, O]]
[[Category: Avraham, O]]