7peg - Revision history

OCA at 18:56, 7 September 2022

2022-09-07T18:56:12Z

←Older revision		Revision as of 18:56, 7 September 2022
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	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/YHCN_BACSU YHCN_BACSU]] Probably contributes, directly or indirectly, to early events in germination (PubMed:28333204). May play a role in spore outgrowth (PubMed:9611260).<ref>PMID:28333204</ref> <ref>PMID:9611260</ref>		[[https://www.uniprot.org/uniprot/YHCN_BACSU YHCN_BACSU]] Probably contributes, directly or indirectly, to early events in germination (PubMed:28333204). May play a role in spore outgrowth (PubMed:9611260).<ref>PMID:28333204</ref> <ref>PMID:9611260</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Components of specialized secretion systems, which span the inner and outer membranes in Gram-negative bacteria, include ring-forming proteins whose oligomerization was proposed to be promoted by domains called RBM for "Ring-Building Motifs". During spore formation in Gram-positive bacteria, a transport system called the SpoIIIA-SpoIIQ complex also assembles in the double membrane that surrounds the forespore following its endocytosis by the mother cell. The presence of RBM domains in some of the SpoIIIA proteins led to the hypothesis that they would assemble into rings connecting the two membranes and form a conduit between the mother cell and forespore. Among them, SpoIIIAG forms homo-oligomeric rings in vitro but the oligomerization of other RBM-containing SpoIIIA proteins, including SpoIIIAH, remains to be demonstrated. In this work, we identified RBM domains in the YhcN/YlaJ family of proteins that are not related to the SpoIIIA-SpoIIQ complex. We solved the crystal structure of YhcN from Bacillus subtilis, which confirmed the presence of a RBM fold, flanked by additional secondary structures. As the protein did not show any oligomerization ability in vitro, we investigated the structural determinants of ring formation in SpoIIIAG, SpoIIIAH and YhcN. We showed that in vitro, the conserved core of RBM domains alone is not sufficient for oligomerization while the beta-barrel forming region in SpoIIIAG forms rings on its own. This work suggests that some RBMs might indeed participate in the assembly of homomeric rings but others might have evolved toward other functions.
		+
		+	Structural insights into ring-building motif domains involved in bacterial sporulation.,Liu B, Chan H, Bauda E, Contreras-Martel C, Bellard L, Villard AM, Mas C, Neumann E, Fenel D, Favier A, Serrano M, Henriques AO, Rodrigues CDA, Morlot C J Struct Biol. 2022 Mar;214(1):107813. doi: 10.1016/j.jsb.2021.107813. Epub 2021 , Nov 19. PMID:34808342<ref>PMID:34808342</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7peg" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 16:48, 7 September 2022

2022-09-07T16:48:04Z

←Older revision		Revision as of 16:48, 7 September 2022
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	==Structure of the sporulation/germination protein YhcN from Bacillus subtilis==		==Structure of the sporulation/germination protein YhcN from Bacillus subtilis==
-	<StructureSection load='7peg' size='340' side='right'caption='[[7peg]]' scene=''>	+	<StructureSection load='7peg' size='340' side='right'caption='[[7peg]], [[Resolution\|resolution]] 1.77Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PEG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PEG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7peg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PEG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PEG FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7peg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7peg OCA], [https://pdbe.org/7peg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7peg RCSB], [https://www.ebi.ac.uk/pdbsum/7peg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7peg ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7peg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7peg OCA], [https://pdbe.org/7peg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7peg RCSB], [https://www.ebi.ac.uk/pdbsum/7peg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7peg ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/YHCN_BACSU YHCN_BACSU]] Probably contributes, directly or indirectly, to early events in germination (PubMed:28333204). May play a role in spore outgrowth (PubMed:9611260).<ref>PMID:28333204</ref> <ref>PMID:9611260</ref>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillus subtilis subsp. subtilis str. 168]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Bauda E]]		[[Category: Bauda E]]

OCA at 04:46, 25 August 2022

2022-08-25T04:46:19Z

←Older revision		Revision as of 04:46, 25 August 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7peg is ~~ON HOLD until Paper Publication~~	+	==Structure of the sporulation/germination protein YhcN from Bacillus subtilis==
-		+	<StructureSection load='7peg' size='340' side='right'caption='[[7peg]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PEG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PEG FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7peg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7peg OCA], [https://pdbe.org/7peg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7peg RCSB], [https://www.ebi.ac.uk/pdbsum/7peg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7peg ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Bauda E]]
		+	[[Category: Bellard L]]
		+	[[Category: Chan H]]
		+	[[Category: Contreras-Martel C]]
		+	[[Category: Favier A]]
		+	[[Category: Fenel D]]
		+	[[Category: Henriques AOH]]
		+	[[Category: Liu B]]
		+	[[Category: Mas C]]
		+	[[Category: Morlot C]]
		+	[[Category: Neumann E]]
		+	[[Category: Rodrigues CDA]]
		+	[[Category: Serrano M]]
		+	[[Category: Villard AM]]

OCA at 07:29, 8 September 2021

2021-09-08T07:29:42Z

←Older revision		Revision as of 07:29, 8 September 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7peg is ON HOLD	+	The entry 7peg is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7peg" [edit=sysop:move=sysop]

2021-08-18T05:02:16Z

Protected "7peg" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:02, 18 August 2021

OCA: New page: '''Unreleased structure''' The entry 7peg is ON HOLD Authors: Description: Category: Unreleased Structures

2021-08-18T05:02:15Z

New page: '''Unreleased structure''' The entry 7peg is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7peg is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]