7pgn - Revision history

OCA at 13:05, 1 February 2024

2024-02-01T13:05:14Z

←Older revision		Revision as of 13:05, 1 February 2024
Line 3:		Line 3:
	<StructureSection load='7pgn' size='340' side='right'caption='[[7pgn]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='7pgn' size='340' side='right'caption='[[7pgn]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7pgn]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PGN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PGN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7pgn]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PGN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PGN FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=GU4:2,3,4,6-TETRA-O-SULFONATO-ALPHA-D-GLUCOPYRANOSE'>GU4</scene><~~/td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.4Å</td></tr>
-	~~<tr id~~='~~NonStdRes'><td class~~=~~"sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res~~:~~]]</b~~></~~td><td class="sblockDat"~~><scene name='pdbligand=YYJ:'>YYJ</scene></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=GU4:2,3,4,6-TETRA-O-SULFONATO-ALPHA-D-GLUCOPYRANOSE'>GU4</scene>, <scene name='pdbligand=PRD_900013:sucrose+octasulfate'>PRD_900013</scene>, <scene name='pdbligand=YYJ:1,3,4,6-tetra-O-sulfo-beta-D-fructofuranose'>YYJ</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7pgn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7pgn OCA], [https://pdbe.org/7pgn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7pgn RCSB], [https://www.ebi.ac.uk/pdbsum/7pgn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7pgn ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7pgn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7pgn OCA], [https://pdbe.org/7pgn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7pgn RCSB], [https://www.ebi.ac.uk/pdbsum/7pgn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7pgn ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/HHIP_HUMAN HHIP_HUMAN]] Modulates hedgehog signaling in several cell types including brain and lung through direct interaction with members of the hedgehog family.<ref>PMID:11472839</ref> <ref>PMID:19561609</ref>	+	[https://www.uniprot.org/uniprot/HHIP_HUMAN HHIP_HUMAN] Modulates hedgehog signaling in several cell types including brain and lung through direct interaction with members of the hedgehog family.<ref>PMID:11472839</ref> <ref>PMID:19561609</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bishop, B]]	+	[[Category: Bishop B]]
-	[[Category: Covey~~, D F~~]]	+	[[Category: Covey DF]]
-	[[Category: Dubey, R]]	+	[[Category: Dubey R]]
-	[[Category: ~~Gilbert, R J.C~~]]	+	[[Category: El Omari K]]
-	[[Category: ~~Griffiths, S C~~]]	+	[[Category: Gilbert RJC]]
-	[[Category: ~~Iverson, E J~~]]	+	[[Category: Griffiths SC]]
-	[[Category: ~~Malinuskas, T~~]]	+	[[Category: Iverson EJ]]
-	[[Category: ~~Omari, K El~~]]	+	[[Category: Malinuskas T]]
-	[[Category: Qian, M]]	+	[[Category: Qian M]]
-	[[Category: Rohatgi, R]]	+	[[Category: Rohatgi R]]
-	[[Category: Schwab~~, R A~~]]	+	[[Category: Schwab RA]]
-	[[Category: Siebold, C]]	+	[[Category: Siebold C]]
-	~~[[Category: Cholesterol]]~~	+
-	~~[[Category: Glycosaminoglycan]]~~	+
-	~~[[Category: Hedgehog]]~~	+
-	~~[[Category: Hhip]]~~	+
-	~~[[Category: Morphogen]]~~	+
-	~~[[Category: Palmitate]]~~	+
-	~~[[Category: Secreted]]~~	+
-	~~[[Category: Signaling protein]]~~	+
-	~~[[Category: Signalling~~]]	+

OCA at 15:40, 22 December 2021

2021-12-22T15:40:27Z

←Older revision		Revision as of 15:40, 22 December 2021
Line 1:		Line 1:

	==HHP-C in complex with glycosaminoglycan mimic SOS==		==HHP-C in complex with glycosaminoglycan mimic SOS==
-	<StructureSection load='7pgn' size='340' side='right'caption='[[7pgn]]' scene=''>	+	<StructureSection load='7pgn' size='340' side='right'caption='[[7pgn]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PGN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PGN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7pgn]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PGN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PGN FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7pgn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7pgn OCA], [https://pdbe.org/7pgn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7pgn RCSB], [https://www.ebi.ac.uk/pdbsum/7pgn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7pgn ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=GU4:2,3,4,6-TETRA-O-SULFONATO-ALPHA-D-GLUCOPYRANOSE'>GU4</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=YYJ:'>YYJ</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7pgn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7pgn OCA], [https://pdbe.org/7pgn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7pgn RCSB], [https://www.ebi.ac.uk/pdbsum/7pgn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7pgn ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/HHIP_HUMAN HHIP_HUMAN]] Modulates hedgehog signaling in several cell types including brain and lung through direct interaction with members of the hedgehog family.<ref>PMID:11472839</ref> <ref>PMID:19561609</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Hedgehog (HH) morphogen signalling, crucial for cell growth and tissue patterning in animals, is initiated by the binding of dually lipidated HH ligands to cell surface receptors. Hedgehog-Interacting Protein (HHIP), the only reported secreted inhibitor of Sonic Hedgehog (SHH) signalling, binds directly to SHH with high nanomolar affinity, sequestering SHH. Here, we report the structure of the HHIP N-terminal domain (HHIP-N) in complex with a glycosaminoglycan (GAG). HHIP-N displays a unique bipartite fold with a GAG-binding domain alongside a Cysteine Rich Domain (CRD). We show that HHIP-N is required to convey full HHIP inhibitory function, likely by interacting with the cholesterol moiety covalently linked to HH ligands, thereby preventing this SHH-attached cholesterol from binding to the HH receptor Patched (PTCH1). We also present the structure of the HHIP C-terminal domain in complex with the GAG heparin. Heparin can bind to both HHIP-N and HHIP-C, thereby inducing clustering at the cell surface and generating a high-avidity platform for SHH sequestration and inhibition. Our data suggest a multimodal mechanism, in which HHIP can bind two specific sites on the SHH morphogen, alongside multiple GAG interactions, to inhibit SHH signalling.
		+
		+	Hedgehog-Interacting Protein is a multimodal antagonist of Hedgehog signalling.,Griffiths SC, Schwab RA, El Omari K, Bishop B, Iverson EJ, Malinauskas T, Dubey R, Qian M, Covey DF, Gilbert RJC, Rohatgi R, Siebold C Nat Commun. 2021 Dec 9;12(1):7171. doi: 10.1038/s41467-021-27475-2. PMID:34887403<ref>PMID:34887403</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7pgn" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bishop B]]	+	[[Category: Bishop, B]]
-	[[Category: Covey DF]]	+	[[Category: Covey, D F]]
-	[[Category: Dubey R]]	+	[[Category: Dubey, R]]
-	[[Category: ~~El Omari K~~]]	+	[[Category: Gilbert, R J.C]]
-	[[Category: ~~Gilbert RJC~~]]	+	[[Category: Griffiths, S C]]
-	[[Category: ~~Griffiths SC~~]]	+	[[Category: Iverson, E J]]
-	[[Category: ~~Iverson EJ~~]]	+	[[Category: Malinuskas, T]]
-	[[Category: ~~Malinuskas T~~]]	+	[[Category: Omari, K El]]
-	[[Category: Qian M]]	+	[[Category: Qian, M]]
-	[[Category: Rohatgi R]]	+	[[Category: Rohatgi, R]]
-	[[Category: Schwab RA]]	+	[[Category: Schwab, R A]]
-	[[Category: Siebold C]]	+	[[Category: Siebold, C]]
		+	[[Category: Cholesterol]]
		+	[[Category: Glycosaminoglycan]]
		+	[[Category: Hedgehog]]
		+	[[Category: Hhip]]
		+	[[Category: Morphogen]]
		+	[[Category: Palmitate]]
		+	[[Category: Secreted]]
		+	[[Category: Signaling protein]]
		+	[[Category: Signalling]]

OCA at 16:48, 15 December 2021

2021-12-15T16:48:54Z

←Older revision		Revision as of 16:48, 15 December 2021
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7pgn ~~is ON HOLD until Paper Publication~~	+	==HHP-C in complex with glycosaminoglycan mimic SOS==
-		+	<StructureSection load='7pgn' size='340' side='right'caption='[[7pgn]]' scene=''>
-	~~Authors~~: ~~Griffiths, S~~.C.~~, Schwab, R~~.A.~~, El Omari, K~~.~~, Bishop, B~~.~~, Iverson, E~~.J.~~, Malinuskas, T~~., ~~Dubey, R~~., ~~Qian, M~~., ~~Covey, D~~.F., ~~Gilbert, R~~.J.C., ~~Rohatgi, R~~.~~, Siebold, C~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PGN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PGN FirstGlance]. <br>
-	~~Description: HHP-C in complex with glycosaminoglycan mimic SOS~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7pgn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7pgn OCA], [https://pdbe.org/7pgn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7pgn RCSB], [https://www.ebi.ac.uk/pdbsum/7pgn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7pgn ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~El Omari, K~~]]	+	__TOC__
-	[[Category: ~~Bishop, B~~]]	+	</StructureSection>
-	[[Category: ~~Schwab,~~ R.A]]	+	[[Category: Large Structures]]
-	[[Category: ~~Gilbert, R.J.C~~]]	+	[[Category: Bishop B]]
-	[[Category: ~~Rohatgi, R~~]]	+	[[Category: Covey DF]]
-	[[Category: ~~Covey, D.F~~]]	+	[[Category: Dubey R]]
-	[[Category: ~~Griffiths, S.C~~]]	+	[[Category: El Omari K]]
-	[[Category: ~~Iverson, E.J~~]]	+	[[Category: Gilbert RJC]]
-	[[Category: ~~Malinuskas, T~~]]	+	[[Category: Griffiths SC]]
-	[[Category: ~~Dubey,~~ R]]	+	[[Category: Iverson EJ]]
-	[[Category: ~~Qian, M~~]]	+	[[Category: Malinuskas T]]
-	[[Category: Siebold, C]]	+	[[Category: Qian M]]
		+	[[Category: Rohatgi R]]
		+	[[Category: Schwab RA]]
		+	[[Category: Siebold C]]

OCA at 08:09, 15 September 2021

2021-09-15T08:09:17Z

←Older revision		Revision as of 08:09, 15 September 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7pgn is ON HOLD	+	The entry 7pgn is ON HOLD until Paper Publication

	Authors: Griffiths, S.C., Schwab, R.A., El Omari, K., Bishop, B., Iverson, E.J., Malinuskas, T., Dubey, R., Qian, M., Covey, D.F., Gilbert, R.J.C., Rohatgi, R., Siebold, C.		Authors: Griffiths, S.C., Schwab, R.A., El Omari, K., Bishop, B., Iverson, E.J., Malinuskas, T., Dubey, R., Qian, M., Covey, D.F., Gilbert, R.J.C., Rohatgi, R., Siebold, C.

OCA: Protected "7pgn" [edit=sysop:move=sysop]

2021-08-25T05:11:24Z

Protected "7pgn" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:11, 25 August 2021

OCA: New page: '''Unreleased structure''' The entry 7pgn is ON HOLD Authors: Griffiths, S.C., Schwab, R.A., El Omari, K., Bishop, B., Iverson, E.J., Malinuskas, T., Dubey, R., Qian, M., Covey, D.F., G...

2021-08-25T05:11:23Z

New page: '''Unreleased structure''' The entry 7pgn is ON HOLD Authors: Griffiths, S.C., Schwab, R.A., El Omari, K., Bishop, B., Iverson, E.J., Malinuskas, T., Dubey, R., Qian, M., Covey, D.F., G...

New page

'''Unreleased structure'''

The entry 7pgn is ON HOLD

Authors: Griffiths, S.C., Schwab, R.A., El Omari, K., Bishop, B., Iverson, E.J., Malinuskas, T., Dubey, R., Qian, M., Covey, D.F., Gilbert, R.J.C., Rohatgi, R., Siebold, C.

Description: HHP-C in complex with glycosaminoglycan mimic SOS
[[Category: Unreleased Structures]]
[[Category: El Omari, K]]
[[Category: Bishop, B]]
[[Category: Schwab, R.A]]
[[Category: Gilbert, R.J.C]]
[[Category: Rohatgi, R]]
[[Category: Covey, D.F]]
[[Category: Griffiths, S.C]]
[[Category: Iverson, E.J]]
[[Category: Malinuskas, T]]
[[Category: Dubey, R]]
[[Category: Qian, M]]
[[Category: Siebold, C]]