7ph4 - Revision history

OCA at 06:23, 26 October 2022

2022-10-26T06:23:46Z

←Older revision		Revision as of 06:23, 26 October 2022
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	==AMP-PNP bound nanodisc reconstituted MsbA with nanobodies, spin-labeled at position T68C==		==AMP-PNP bound nanodisc reconstituted MsbA with nanobodies, spin-labeled at position T68C==
-	<StructureSection load='7ph4' size='340' side='right'caption='[[7ph4]]' scene=''>	+	<StructureSection load='7ph4' size='340' side='right'caption='[[7ph4]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PH4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PH4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7ph4]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12] and [https://en.wikipedia.org/wiki/Vicugna_pacos Vicugna pacos]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PH4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PH4 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ph4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ph4 OCA], [https://pdbe.org/7ph4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ph4 RCSB], [https://www.ebi.ac.uk/pdbsum/7ph4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ph4 ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=88T:(1~{R},4~{R},11~{S},14~{S},19~{Z})-19-[2-[2,5-bis(oxidanylidene)pyrrolidin-1-yl]ethylimino]-7,8,17,18-tetraoxa-1,4,11,14-tetrazatricyclo[12.6.2.2^{4,11}]tetracosane-6,9,16-trione'>88T</scene>, <scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=GD:GADOLINIUM+ATOM'>GD</scene>, <scene name='pdbligand=LMT:DODECYL-BETA-D-MALTOSIDE'>LMT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ph4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ph4 OCA], [https://pdbe.org/7ph4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ph4 RCSB], [https://www.ebi.ac.uk/pdbsum/7ph4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ph4 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/MSBA_ECOLI MSBA_ECOLI] Involved in lipid A export and possibly also in glycerophospholipid export and for biogenesis of the outer membrane. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Membrane proteins are currently investigated after detergent extraction from native cellular membranes and reconstitution into artificial liposomes or nanodiscs, thereby removing them from their physiological environment. However, to truly understand the biophysical properties of membrane proteins in a physiological environment, they must be investigated within living cells. Here, we used a spin-labeled nanobody to interrogate the conformational cycle of the ABC transporter MsbA by double electron-electron resonance. Unexpectedly, the wide inward-open conformation of MsbA, commonly considered a nonphysiological state, was found to be prominently populated in Escherichia coli cells. Molecular dynamics simulations revealed that extensive lateral portal opening is essential to provide access of its large natural substrate core lipid A to the binding cavity. Our work paves the way to investigate the conformational landscape of membrane proteins in cells.
		+
		+	The ABC transporter MsbA adopts the wide inward-open conformation in E. coli cells.,Galazzo L, Meier G, Januliene D, Parey K, De Vecchis D, Striednig B, Hilbi H, Schafer LV, Kuprov I, Moeller A, Bordignon E, Seeger MA Sci Adv. 2022 Oct 14;8(41):eabn6845. doi: 10.1126/sciadv.abn6845. Epub 2022 Oct, 12. PMID:36223470<ref>PMID:36223470</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7ph4" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Escherichia coli K-12]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Vicugna pacos]]
	[[Category: Bordignon E]]		[[Category: Bordignon E]]
	[[Category: Galazzo L]]		[[Category: Galazzo L]]

OCA at 10:02, 31 August 2022

2022-08-31T10:02:52Z

←Older revision		Revision as of 10:02, 31 August 2022
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-	====	+	==AMP-PNP bound nanodisc reconstituted MsbA with nanobodies, spin-labeled at position T68C==
	<StructureSection load='7ph4' size='340' side='right'caption='[[7ph4]]' scene=''>		<StructureSection load='7ph4' size='340' side='right'caption='[[7ph4]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PH4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PH4 FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ph4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ph4 OCA], [https://pdbe.org/7ph4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ph4 RCSB], [https://www.ebi.ac.uk/pdbsum/7ph4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ph4 ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ph4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ph4 OCA], [https://pdbe.org/7ph4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ph4 RCSB], [https://www.ebi.ac.uk/pdbsum/7ph4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ph4 ProSAT]</span></td></tr>
	</table>		</table>
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Bordignon E]]
		+	[[Category: Galazzo L]]
		+	[[Category: Hilbi H]]
		+	[[Category: Januliene D]]
		+	[[Category: Kuprov I]]
		+	[[Category: Meier G]]
		+	[[Category: Moeller A]]
		+	[[Category: Parey K]]
		+	[[Category: Schaefer LV]]
		+	[[Category: Seeger MA]]
		+	[[Category: Striednig B]]
		+	[[Category: Vecchis D]]

OCA at 04:47, 25 August 2022

2022-08-25T04:47:04Z

←Older revision		Revision as of 04:47, 25 August 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7ph4 ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7ph4' size='340' side='right'caption='[[7ph4]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ph4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ph4 OCA], [https://pdbe.org/7ph4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ph4 RCSB], [https://www.ebi.ac.uk/pdbsum/7ph4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ph4 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 09:13, 5 January 2022

2022-01-05T09:13:15Z

←Older revision		Revision as of 09:13, 5 January 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7ph4 is ON HOLD	+	The entry 7ph4 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA at 04:59, 1 December 2021

2021-12-01T04:59:58Z

←Older revision		Revision as of 04:59, 1 December 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7ph4 is ON HOLD ~~until sometime in the future~~	+	The entry 7ph4 is ON HOLD

	Authors:		Authors:

OCA at 12:31, 27 October 2021

2021-10-27T12:31:59Z

←Older revision		Revision as of 12:31, 27 October 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7ph4 is ON HOLD	+	The entry 7ph4 is ON HOLD until sometime in the future

	Authors:		Authors:

OCA at 07:31, 8 September 2021

2021-09-08T07:31:03Z

←Older revision		Revision as of 07:31, 8 September 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7ph4 is ON HOLD ~~until sometime in the future~~	+	The entry 7ph4 is ON HOLD

	Authors:		Authors:

OCA: Protected "7ph4" [edit=sysop:move=sysop]

2021-08-25T05:12:04Z

Protected "7ph4" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:12, 25 August 2021

OCA: New page: '''Unreleased structure''' The entry 7ph4 is ON HOLD until sometime in the future Authors: Description: Category: Unreleased Structures

2021-08-25T05:12:03Z

New page: '''Unreleased structure''' The entry 7ph4 is ON HOLD until sometime in the future Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7ph4 is ON HOLD until sometime in the future

Authors:

Description:
[[Category: Unreleased Structures]]