OCA at 12:05, 1 February 2023

2023-02-01T12:05:31Z

←Older revision		Revision as of 12:05, 1 February 2023
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-	====	+	==Cryo-EM structure of ShCas12k in complex with a sgRNA and a dsDNA target==
-	<StructureSection load='7pla' size='340' side='right'caption='[[7pla]]' scene=''>	+	<StructureSection load='7pla' size='340' side='right'caption='[[7pla]], [[Resolution\|resolution]] 3.04Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7pla]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Scytonema_hofmannii Scytonema hofmannii] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PLA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PLA FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7pla FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7pla OCA], [https://pdbe.org/7pla PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7pla RCSB], [https://www.ebi.ac.uk/pdbsum/7pla PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7pla ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7pla FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7pla OCA], [https://pdbe.org/7pla PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7pla RCSB], [https://www.ebi.ac.uk/pdbsum/7pla PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7pla ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A8X6EH11_9CYAN A0A8X6EH11_9CYAN]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Canonical CRISPR-Cas systems provide adaptive immunity against mobile genetic elements(1). However, type I-F, I-B and V-K systems have been adopted by Tn7-like transposons to direct RNA-guided transposon insertion(2-7). Type V-K CRISPR-associated transposons rely on the pseudonuclease Cas12k, the transposase TnsB, the AAA+ ATPase TnsC and the zinc-finger protein TniQ(7), but the molecular mechanism of RNA-directed DNA transposition has remained elusive. Here we report cryo-electron microscopic structures of a Cas12k-guide RNA-target DNA complex and a DNA-bound, polymeric TnsC filament from the CRISPR-associated transposon system of the photosynthetic cyanobacterium Scytonema hofmanni. The Cas12k complex structure reveals an intricate guide RNA architecture and critical interactions mediating RNA-guided target DNA recognition. TnsC helical filament assembly is ATP-dependent and accompanied by structural remodelling of the bound DNA duplex. In vivo transposition assays corroborate key features of the structures, and biochemical experiments show that TniQ restricts TnsC polymerization, while TnsB interacts directly with TnsC filaments to trigger their disassembly upon ATP hydrolysis. Together, these results suggest that RNA-directed target selection by Cas12k primes TnsC polymerization and DNA remodelling, generating a recruitment platform for TnsB to catalyse site-specific transposon insertion. Insights from this work will inform the development of CRISPR-associated transposons as programmable site-specific gene insertion tools.
		+
		+	Target site selection and remodelling by type V CRISPR-transposon systems.,Querques I, Schmitz M, Oberli S, Chanez C, Jinek M Nature. 2021 Nov;599(7885):497-502. doi: 10.1038/s41586-021-04030-z. Epub 2021, Nov 10. PMID:34759315<ref>PMID:34759315</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7pla" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Transposase 3D structures\|Transposase 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Scytonema hofmannii]]
		+	[[Category: Synthetic construct]]
		+	[[Category: Jinek M]]
		+	[[Category: Schmitz M]]

OCA at 06:21, 1 December 2021

2021-12-01T06:21:53Z

←Older revision		Revision as of 06:21, 1 December 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7pla ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7pla' size='340' side='right'caption='[[7pla]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7pla FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7pla OCA], [https://pdbe.org/7pla PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7pla RCSB], [https://www.ebi.ac.uk/pdbsum/7pla PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7pla ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 04:04, 6 October 2021

2021-10-06T04:04:09Z

←Older revision		Revision as of 04:04, 6 October 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7pla is ON HOLD	+	The entry 7pla is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7pla" [edit=sysop:move=sysop]

2021-09-08T07:36:16Z

Protected "7pla" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:36, 8 September 2021

OCA: New page: '''Unreleased structure''' The entry 7pla is ON HOLD Authors: Description: Category: Unreleased Structures

2021-09-08T07:36:15Z

New page: '''Unreleased structure''' The entry 7pla is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7pla is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7pla - Revision history

OCA at 12:05, 1 February 2023

OCA at 06:21, 1 December 2021

OCA at 04:04, 6 October 2021

OCA: Protected "7pla" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7pla is ON HOLD Authors: Description: Category: Unreleased Structures