7pvm - Revision history

OCA at 11:19, 14 June 2023

2023-06-14T11:19:36Z

←Older revision		Revision as of 11:19, 14 June 2023
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	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/G0S058_CHATD G0S058_CHATD]] Possesses 5'->3' exoribonuclease activity. May promote termination of transcription by RNA polymerase II.[PIRNR:PIRNR037239] Possesses 5'->3' exoribonuclease activity. Required for the processing of nuclear mRNA and rRNA precursors. May promote termination of transcription by RNA polymerase II.[ARBA:ARBA00025537]	+	[https://www.uniprot.org/uniprot/G0S058_CHATD G0S058_CHATD] Possesses 5'->3' exoribonuclease activity. May promote termination of transcription by RNA polymerase II.[PIRNR:PIRNR037239] Possesses 5'->3' exoribonuclease activity. Required for the processing of nuclear mRNA and rRNA precursors. May promote termination of transcription by RNA polymerase II.[ARBA:ARBA00025537]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 18:56, 7 September 2022

2022-09-07T18:56:19Z

←Older revision		Revision as of 18:56, 7 September 2022
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	== Function ==		== Function ==
	[[https://www.uniprot.org/uniprot/G0S058_CHATD G0S058_CHATD]] Possesses 5'->3' exoribonuclease activity. May promote termination of transcription by RNA polymerase II.[PIRNR:PIRNR037239] Possesses 5'->3' exoribonuclease activity. Required for the processing of nuclear mRNA and rRNA precursors. May promote termination of transcription by RNA polymerase II.[ARBA:ARBA00025537]		[[https://www.uniprot.org/uniprot/G0S058_CHATD G0S058_CHATD]] Possesses 5'->3' exoribonuclease activity. May promote termination of transcription by RNA polymerase II.[PIRNR:PIRNR037239] Possesses 5'->3' exoribonuclease activity. Required for the processing of nuclear mRNA and rRNA precursors. May promote termination of transcription by RNA polymerase II.[ARBA:ARBA00025537]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Nuclear magnetic resonance (NMR) methods that quantitatively probe motions on molecular and atomic levels have propelled the understanding of biomolecular processes for which static structures cannot provide a satisfactory description. In this work, we studied the structure and dynamics of the essential 100-kDa eukaryotic 5'-->3' exoribonuclease Xrn2. A combination of complementary fluorine and methyl-TROSY NMR spectroscopy reveals that the apo enzyme is highly dynamic around the catalytic center. These observed dynamics are in agreement with a transition of the enzyme from the ground state into a catalytically competent state. We show that the conformational equilibrium in Xrn2 shifts substantially toward the active state in the presence of substrate and magnesium. Finally, our data reveal that the dynamics in Xrn2 correlate with the RNA degradation rate, as a mutation that attenuates motions also affects catalytic activity. In that light, our results stress the importance of studies that go beyond static structural information.
		+
		+	Observation of conformational changes that underlie the catalytic cycle of Xrn2.,Overbeck JH, Stelzig D, Fuchs AL, Wurm JP, Sprangers R Nat Chem Biol. 2022 Aug 25. pii: 10.1038/s41589-022-01111-6. doi:, 10.1038/s41589-022-01111-6. PMID:36008487<ref>PMID:36008487</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7pvm" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 16:48, 7 September 2022

2022-09-07T16:48:06Z

←Older revision		Revision as of 16:48, 7 September 2022
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-	==Xrn2 zinc finger==	+	==NMR structure of the C. thermophilum Xrn2 zinc finger==
	<StructureSection load='7pvm' size='340' side='right'caption='[[7pvm]]' scene=''>		<StructureSection load='7pvm' size='340' side='right'caption='[[7pvm]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full ~~crystallographic~~ information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PVM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PVM FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7pvm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Chaetomium_thermophilum_var._thermophilum_DSM_1495 Chaetomium thermophilum var. thermophilum DSM 1495]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PVM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PVM FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7pvm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7pvm OCA], [https://pdbe.org/7pvm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7pvm RCSB], [https://www.ebi.ac.uk/pdbsum/7pvm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7pvm ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7pvm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7pvm OCA], [https://pdbe.org/7pvm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7pvm RCSB], [https://www.ebi.ac.uk/pdbsum/7pvm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7pvm ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/G0S058_CHATD G0S058_CHATD]] Possesses 5'->3' exoribonuclease activity. May promote termination of transcription by RNA polymerase II.[PIRNR:PIRNR037239] Possesses 5'->3' exoribonuclease activity. Required for the processing of nuclear mRNA and rRNA precursors. May promote termination of transcription by RNA polymerase II.[ARBA:ARBA00025537]
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Chaetomium thermophilum var. thermophilum DSM 1495]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Overbeck JH]]		[[Category: Overbeck JH]]
	[[Category: Sprangers R]]		[[Category: Sprangers R]]
	[[Category: Wurm JP]]		[[Category: Wurm JP]]

OCA at 16:12, 6 July 2022

2022-07-06T16:12:41Z

←Older revision		Revision as of 16:12, 6 July 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7pvm is ~~ON HOLD until 2023~~-10-05	+	==Xrn2 zinc finger==
-		+	<StructureSection load='7pvm' size='340' side='right'caption='[[7pvm]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7PVM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7PVM FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7pvm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7pvm OCA], [https://pdbe.org/7pvm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7pvm RCSB], [https://www.ebi.ac.uk/pdbsum/7pvm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7pvm ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Overbeck JH]]
		+	[[Category: Sprangers R]]
		+	[[Category: Wurm JP]]

OCA at 06:27, 22 June 2022

2022-06-22T06:27:13Z

←Older revision		Revision as of 06:27, 22 June 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7pvm is ON HOLD until ~~Oct 05~~ 2023	+	The entry 7pvm is ON HOLD until 2023-10-05

	Authors:		Authors:

OCA at 12:41, 3 November 2021

2021-11-03T12:41:45Z

←Older revision		Revision as of 12:41, 3 November 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7pvm is ON HOLD	+	The entry 7pvm is ON HOLD until Oct 05 2023

	Authors:		Authors:

OCA: Protected "7pvm" [edit=sysop:move=sysop]

2021-10-13T10:15:43Z

Protected "7pvm" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:15, 13 October 2021

OCA: New page: '''Unreleased structure''' The entry 7pvm is ON HOLD Authors: Description: Category: Unreleased Structures

2021-10-13T10:15:41Z

New page: '''Unreleased structure''' The entry 7pvm is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7pvm is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]