7q53 - Revision history

OCA at 20:02, 16 November 2022

2022-11-16T20:02:55Z

←Older revision		Revision as of 20:02, 16 November 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7q53 is ~~ON HOLD until Paper~~ Publication	+	==Single Particle Cryo-EM structure of photosynthetic A2B2 glyceraldehyde 3-phosphate dehydrogenase from Spinacia oleracia==
		+	<StructureSection load='7q53' size='340' side='right'caption='[[7q53]], [[Resolution\|resolution]] 6.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[7q53]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Spinacia_oleracea Spinacia oleracea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7Q53 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7Q53 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7q53 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7q53 OCA], [https://pdbe.org/7q53 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7q53 RCSB], [https://www.ebi.ac.uk/pdbsum/7q53 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7q53 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/G3PB_SPIOL G3PB_SPIOL]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Oxygenic phototrophs perform carbon fixation through the Calvin-Benson cycle. Different mechanisms adjust the cycle and the light-harvesting reactions to rapid environmental changes. Photosynthetic glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is a key enzyme in the cycle. In land plants, different photosynthetic GAPDHs exist: the most abundant isoform is formed by A2B2 heterotetramers and the least abundant by A4 homotetramers. Regardless of the subunit composition, GAPDH is the major consumer of photosynthetic NADPH and its activity is strictly regulated. While A4-GAPDH is regulated by CP12, AB-GAPDH is autonomously regulated through the C-terminal extension (CTE) of its B subunits. Reversible inhibition of AB-GAPDH occurs via the oxidation of a cysteine pair located in the CTE and the substitution of NADP(H) with NAD(H) in the cofactor-binding site. These combined conditions lead to a change in the oligomerization state and enzyme inhibition. SEC-SAXS and single-particle cryo-EM analysis were applied to reveal the structural basis of this regulatory mechanism. Both approaches revealed that spinach (A2B2)n-GAPDH oligomers with n = 1, 2, 4 and 5 co-exist in a dynamic system. B subunits mediate the contacts between adjacent tetramers in A4B4 and A8B8 oligomers. The CTE of each B subunit penetrates into the active site of a B subunit of the adjacent tetramer, which in turn moves its CTE in the opposite direction, effectively preventing the binding of the substrate 1,3-bisphosphoglycerate in the B subunits. The whole mechanism is made possible, and eventually controlled, by pyridine nucleotides. In fact, NAD(H), by removing NADP(H) from A subunits, allows the entrance of the CTE into the active site of the B subunit, hence stabilizing inhibited oligomers.

-	~~Authors: Marotta~~, R., ~~Fermani~~, S., ~~Sparla~~, F., ~~Trost~~, P., ~~Del Giudice~~, A.	+	Unravelling the regulation pathway of photosynthetic AB-GAPDH.,Marotta R, Del Giudice A, Gurrieri L, Fanti S, Swuec P, Galantini L, Falini G, Trost P, Fermani S, Sparla F Acta Crystallogr D Struct Biol. 2022 Nov 1;78(Pt 11):1399-1411. doi:, 10.1107/S2059798322010014. Epub 2022 Oct 27. PMID:36322422<ref>PMID:36322422</ref>

-	~~Description: Single Particle Cryo-EM structure~~ of ~~photosynthetic A2B2 glyceraldehyde 3~~-~~phosphate dehydrogenase from Spinacia oleracia~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: ~~Fermani, S~~]]	+	<div class="pdbe-citations 7q53" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Sparla, F~~]]	+	== References ==
-	[[Category: ~~Trost, P~~]]	+	<references/>
-	[[Category: ~~Del Giudice, A~~]]	+	__TOC__
-	[[Category: ~~Marotta, R~~]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Spinacia oleracea]]
		+	[[Category: Del Giudice A]]
		+	[[Category: Fermani S]]
		+	[[Category: Marotta R]]
		+	[[Category: Sparla F]]
		+	[[Category: Trost P]]

OCA at 03:49, 15 June 2022

2022-06-15T03:49:24Z

←Older revision		Revision as of 03:49, 15 June 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7q53 is ON HOLD	+	The entry 7q53 is ON HOLD until Paper Publication

	Authors: Marotta, R., Fermani, S., Sparla, F., Trost, P., Del Giudice, A.		Authors: Marotta, R., Fermani, S., Sparla, F., Trost, P., Del Giudice, A.
Line 7:		Line 7:
	Description: Single Particle Cryo-EM structure of photosynthetic A2B2 glyceraldehyde 3-phosphate dehydrogenase from Spinacia oleracia		Description: Single Particle Cryo-EM structure of photosynthetic A2B2 glyceraldehyde 3-phosphate dehydrogenase from Spinacia oleracia
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
		+	[[Category: Fermani, S]]
		+	[[Category: Sparla, F]]
	[[Category: Trost, P]]		[[Category: Trost, P]]
	[[Category: Del Giudice, A]]		[[Category: Del Giudice, A]]
-	[[Category: Fermani, S]]
	[[Category: Marotta, R]]		[[Category: Marotta, R]]
-	[[Category: Sparla, F]]

OCA at 03:48, 8 June 2022

2022-06-08T03:48:28Z

←Older revision		Revision as of 03:48, 8 June 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7q53 is ON HOLD ~~until sometime in the future~~	+	The entry 7q53 is ON HOLD

	Authors: Marotta, R., Fermani, S., Sparla, F., Trost, P., Del Giudice, A.		Authors: Marotta, R., Fermani, S., Sparla, F., Trost, P., Del Giudice, A.
Line 7:		Line 7:
	Description: Single Particle Cryo-EM structure of photosynthetic A2B2 glyceraldehyde 3-phosphate dehydrogenase from Spinacia oleracia		Description: Single Particle Cryo-EM structure of photosynthetic A2B2 glyceraldehyde 3-phosphate dehydrogenase from Spinacia oleracia
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Fermani, S]]
-	[[Category: Sparla, F]]
	[[Category: Trost, P]]		[[Category: Trost, P]]
	[[Category: Del Giudice, A]]		[[Category: Del Giudice, A]]
		+	[[Category: Fermani, S]]
	[[Category: Marotta, R]]		[[Category: Marotta, R]]
		+	[[Category: Sparla, F]]

OCA at 10:20, 27 April 2022

2022-04-27T10:20:04Z

←Older revision		Revision as of 10:20, 27 April 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7q53 is ON HOLD	+	The entry 7q53 is ON HOLD until sometime in the future

	Authors: Marotta, R., Fermani, S., Sparla, F., Trost, P., Del Giudice, A.		Authors: Marotta, R., Fermani, S., Sparla, F., Trost, P., Del Giudice, A.

OCA at 08:27, 8 December 2021

2021-12-08T08:27:27Z

←Older revision		Revision as of 08:27, 8 December 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7q53 is ON HOLD ~~until sometime in the future~~	+	The entry 7q53 is ON HOLD

	Authors: Marotta, R., Fermani, S., Sparla, F., Trost, P., Del Giudice, A.		Authors: Marotta, R., Fermani, S., Sparla, F., Trost, P., Del Giudice, A.

OCA at 05:02, 1 December 2021

2021-12-01T05:02:43Z

←Older revision		Revision as of 05:02, 1 December 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7q53 is ON HOLD	+	The entry 7q53 is ON HOLD until sometime in the future

	Authors: Marotta, R., Fermani, S., Sparla, F., Trost, P., Del Giudice, A.		Authors: Marotta, R., Fermani, S., Sparla, F., Trost, P., Del Giudice, A.
Line 7:		Line 7:
	Description: Single Particle Cryo-EM structure of photosynthetic A2B2 glyceraldehyde 3-phosphate dehydrogenase from Spinacia oleracia		Description: Single Particle Cryo-EM structure of photosynthetic A2B2 glyceraldehyde 3-phosphate dehydrogenase from Spinacia oleracia
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
		+	[[Category: Fermani, S]]
	[[Category: Sparla, F]]		[[Category: Sparla, F]]
	[[Category: Trost, P]]		[[Category: Trost, P]]
	[[Category: Del Giudice, A]]		[[Category: Del Giudice, A]]
-	[[Category: Fermani, S]]
	[[Category: Marotta, R]]		[[Category: Marotta, R]]

OCA: Protected "7q53" [edit=sysop:move=sysop]

2021-11-10T05:11:42Z

Protected "7q53" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:11, 10 November 2021

OCA: New page: '''Unreleased structure''' The entry 7q53 is ON HOLD Authors: Marotta, R., Fermani, S., Sparla, F., Trost, P., Del Giudice, A. Description: Single Particle Cryo-EM structure of photosy...

2021-11-10T05:11:41Z

New page: '''Unreleased structure''' The entry 7q53 is ON HOLD Authors: Marotta, R., Fermani, S., Sparla, F., Trost, P., Del Giudice, A. Description: Single Particle Cryo-EM structure of photosy...

New page

'''Unreleased structure'''

The entry 7q53 is ON HOLD

Authors: Marotta, R., Fermani, S., Sparla, F., Trost, P., Del Giudice, A.

Description: Single Particle Cryo-EM structure of photosynthetic A2B2 glyceraldehyde 3-phosphate dehydrogenase from Spinacia oleracia
[[Category: Unreleased Structures]]
[[Category: Sparla, F]]
[[Category: Trost, P]]
[[Category: Del Giudice, A]]
[[Category: Fermani, S]]
[[Category: Marotta, R]]