OCA at 04:11, 8 September 2022

2022-09-08T04:11:44Z

←Older revision		Revision as of 04:11, 8 September 2022
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	==Infectious mouse-adapted RML scrapie prion fibril purified from terminally-infected mouse brains==		==Infectious mouse-adapted RML scrapie prion fibril purified from terminally-infected mouse brains==
-	<StructureSection load='7qig' size='340' side='right'caption='[[7qig]]' scene=''>	+	<StructureSection load='7qig' size='340' side='right'caption='[[7qig]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7QIG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7QIG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7qig]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7QIG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7QIG FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qig FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qig OCA], [https://pdbe.org/7qig PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qig RCSB], [https://www.ebi.ac.uk/pdbsum/7qig PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qig ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qig FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qig OCA], [https://pdbe.org/7qig PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qig RCSB], [https://www.ebi.ac.uk/pdbsum/7qig PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qig ProSAT]</span></td></tr>
	</table>		</table>
		+	== Disease ==
		+	[[https://www.uniprot.org/uniprot/PRIO_MOUSE PRIO_MOUSE]] Note=Found in high quantity in the brain of humans and animals infected with degenerative neurological diseases such as kuru, Creutzfeldt-Jakob disease (CJD), Gerstmann-Straussler syndrome (GSS), scrapie, bovine spongiform encephalopathy (BSE), transmissible mink encephalopathy (TME), etc.
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/PRIO_MOUSE PRIO_MOUSE]] May play a role in neuronal development and synaptic plasticity. May be required for neuronal myelin sheath maintenance. May play a role in iron uptake and iron homeostasis. Soluble oligomers are toxic to cultured neuroblastoma cells and induce apoptosis (in vitro) (By similarity). Association with GPC1 (via its heparan sulfate chains) targets PRNP to lipid rafts. Also provides Cu(2+) or ZN(2+) for the ascorbate-mediated GPC1 deaminase degradation of its heparan sulfate side chains.<ref>PMID:12732622</ref> <ref>PMID:16492732</ref> <ref>PMID:19242475</ref> <ref>PMID:19568430</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Mammalian prions propagate as distinct strains and are composed of multichain assemblies of misfolded host-encoded prion protein (PrP). Here, we present a near-atomic resolution cryo-EM structure of PrP fibrils present in highly infectious prion rod preparations isolated from the brains of RML prion-infected mice. We found that prion rods comprise single-protofilament helical amyloid fibrils that coexist with twisted pairs of the same protofilaments. Each rung of the protofilament is formed by a single PrP monomer with the ordered core comprising PrP residues 94-225, which folds to create two asymmetric lobes with the N-linked glycans and the glycosylphosphatidylinositol anchor projecting from the C-terminal lobe. The overall architecture is comparable to that of recently reported PrP fibrils isolated from the brain of hamsters infected with the 263K prion strain. However, there are marked conformational variations that could result from differences in PrP sequence and/or represent distinguishing features of the distinct prion strains.
		+
		+	2.7 A cryo-EM structure of ex vivo RML prion fibrils.,Manka SW, Zhang W, Wenborn A, Betts J, Joiner S, Saibil HR, Collinge J, Wadsworth JDF Nat Commun. 2022 Jul 13;13(1):4004. doi: 10.1038/s41467-022-30457-7. PMID:35831275<ref>PMID:35831275</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7qig" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Mus musculus]]
	[[Category: Betts J]]		[[Category: Betts J]]
	[[Category: Collinge J]]		[[Category: Collinge J]]

OCA at 18:02, 27 July 2022

2022-07-27T18:02:19Z

←Older revision		Revision as of 18:02, 27 July 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7qig is ~~ON HOLD until Paper Publication~~	+	==Infectious mouse-adapted RML scrapie prion fibril purified from terminally-infected mouse brains==
-		+	<StructureSection load='7qig' size='340' side='right'caption='[[7qig]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7QIG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7QIG FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7qig FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7qig OCA], [https://pdbe.org/7qig PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7qig RCSB], [https://www.ebi.ac.uk/pdbsum/7qig PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7qig ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Betts J]]
		+	[[Category: Collinge J]]
		+	[[Category: Joiner S]]
		+	[[Category: Manka SW]]
		+	[[Category: Saibil HR]]
		+	[[Category: Wadsworth JDF]]
		+	[[Category: Wenborn A]]
		+	[[Category: Zhang W]]

OCA at 05:46, 19 January 2022

2022-01-19T05:46:38Z

←Older revision		Revision as of 05:46, 19 January 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7qig is ON HOLD	+	The entry 7qig is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7qig" [edit=sysop:move=sysop]

2021-12-22T13:57:17Z

Protected "7qig" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:57, 22 December 2021

OCA: New page: '''Unreleased structure''' The entry 7qig is ON HOLD Authors: Description: Category: Unreleased Structures

2021-12-22T13:57:16Z

New page: '''Unreleased structure''' The entry 7qig is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7qig is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7qig - Revision history

OCA at 04:11, 8 September 2022

OCA at 18:02, 27 July 2022

OCA at 05:46, 19 January 2022

OCA: Protected "7qig" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7qig is ON HOLD Authors: Description: Category: Unreleased Structures