OCA at 16:37, 18 October 2023

2023-10-18T16:37:53Z

←Older revision		Revision as of 16:37, 18 October 2023
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-	====	+	==TNA polymerase, n+2 product==
-	<StructureSection load='7rsu' size='340' side='right'caption='[[7rsu]]' scene=''>	+	<StructureSection load='7rsu' size='340' side='right'caption='[[7rsu]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7rsu]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermococcus_kodakarensis Thermococcus kodakarensis] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7RSU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7RSU FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7rsu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7rsu OCA], [https://pdbe.org/7rsu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7rsu RCSB], [https://www.ebi.ac.uk/pdbsum/7rsu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7rsu ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.1Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FA2:5-(6-AMINO-9H-PURIN-9-YL)-4-HYDROXYTETRAHYDROFURAN-3-YL+DIHYDROGEN+PHOSPHATE'>FA2</scene>, <scene name='pdbligand=TFT:(L)-ALPHA-THREOFURANOSYL-THYMINE-3-MONOPHOSPHATE'>TFT</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7rsu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7rsu OCA], [https://pdbe.org/7rsu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7rsu RCSB], [https://www.ebi.ac.uk/pdbsum/7rsu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7rsu ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/DPOL_THEKO DPOL_THEKO] Intein encoded endonucleases are thought to mediate intein mobility by site-specific recombination initiated by endonuclease cleavage at the "homing site" in gene that lack the intein. PI-PkoI recognizes 5'-GATTTAGATCCCTGTACC-3' and PI-PkoII recognizes 5'-CAGCTACTACGGTTAC-3'.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Expanding the chemical space of evolvable non-natural genetic polymers (XNAs) to include functional groups that enhance protein target binding affinity offers a promising route to therapeutic aptamers with high biological stability. Here we describe the chemical synthesis and polymerase recognition of 10 chemically diverse functional groups introduced at the C-5 position of alpha-l-threofuranosyl uridine nucleoside triphosphate (tUTP). We show that the set of tUTP substrates is universally recognized by the laboratory-evolved polymerase Kod-RSGA. Insights into the mechanism of TNA synthesis were obtained from a high-resolution X-ray crystal structure of the postcatalytic complex bound to the primer-template duplex. A structural analysis reveals a large cavity in the enzyme active site that can accommodate the side chain of C-5-modified tUTP substrates. Our findings expand the chemical space of evolvable nucleic acid systems by providing a synthetic route to artificial genetic polymers that are uniformly modified with diversity-enhancing functional groups.
		+
		+	Synthesis and Polymerase Recognition of Threose Nucleic Acid Triphosphates Equipped with Diverse Chemical Functionalities.,Li Q, Maola VA, Chim N, Hussain J, Lozoya-Colinas A, Chaput JC J Am Chem Soc. 2021 Oct 27;143(42):17761-17768. doi: 10.1021/jacs.1c08649. Epub , 2021 Oct 12. PMID:34637287<ref>PMID:34637287</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7rsu" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[DNA polymerase 3D structures\|DNA polymerase 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Synthetic construct]]
		+	[[Category: Thermococcus kodakarensis]]
		+	[[Category: Chaput J]]
		+	[[Category: Chim N]]
		+	[[Category: Maola V]]

OCA at 14:14, 27 October 2021

2021-10-27T14:14:16Z

←Older revision		Revision as of 14:14, 27 October 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 7rsu ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7rsu' size='340' side='right'caption='[[7rsu]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7rsu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7rsu OCA], [https://pdbe.org/7rsu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7rsu RCSB], [https://www.ebi.ac.uk/pdbsum/7rsu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7rsu ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 10:20, 13 October 2021

2021-10-13T10:20:52Z

←Older revision		Revision as of 10:20, 13 October 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7rsu is ON HOLD	+	The entry 7rsu is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7rsu" [edit=sysop:move=sysop]

2021-08-18T05:11:56Z

Protected "7rsu" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:11, 18 August 2021

OCA: New page: '''Unreleased structure''' The entry 7rsu is ON HOLD Authors: Description: Category: Unreleased Structures

2021-08-18T05:11:55Z

New page: '''Unreleased structure''' The entry 7rsu is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7rsu is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7rsu - Revision history

OCA at 16:37, 18 October 2023

OCA at 14:14, 27 October 2021

OCA at 10:20, 13 October 2021

OCA: Protected "7rsu" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7rsu is ON HOLD Authors: Description: Category: Unreleased Structures