7s57 - Revision history

OCA at 16:44, 18 October 2023

2023-10-18T16:44:24Z

←Older revision		Revision as of 16:44, 18 October 2023
Line 3:		Line 3:
	<StructureSection load='7s57' size='340' side='right'caption='[[7s57]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='7s57' size='340' side='right'caption='[[7s57]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7s57]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7S57 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7S57 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7s57]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pyogenes Streptococcus pyogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7S57 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7S57 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7s57 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7s57 OCA], [https://pdbe.org/7s57 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7s57 RCSB], [https://www.ebi.ac.uk/pdbsum/7s57 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7s57 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.7Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7s57 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7s57 OCA], [https://pdbe.org/7s57 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7s57 RCSB], [https://www.ebi.ac.uk/pdbsum/7s57 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7s57 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q99ZN4_STRP1 Q99ZN4_STRP1]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Amacher, J F~~]]	+	[[Category: Streptococcus pyogenes]]
-	[[Category: ~~Antos, J M~~]]	+	[[Category: Amacher JF]]
-	[[Category: ~~Gao, M~~]]	+	[[Category: Antos JM]]
-	[[Category: ~~Johnson, D A~~]]	+	[[Category: Gao M]]
-	[[Category: ~~Svendsen, J E~~]]	+	[[Category: Johnson DA]]
-	[[Category: ~~Eight-stranded beta barrel]]~~	+	[[Category: Svendsen JE]]
-	~~[[Category: Housekeeping sortase]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Sortase]]~~	+
-	~~[[Category: Sortase-fold]]~~	+
-	~~[[Category: Surface protein]]~~	+
-	~~[[Category: Transpeptidase~~]]	+

OCA at 07:32, 2 March 2022

2022-03-02T07:32:24Z

←Older revision		Revision as of 07:32, 2 March 2022
Line 1:		Line 1:

	==Structure of Sortase A from Streptococcus pyogenes with the b7-b8 loop sequence of Enterococcus faecalis Sortase A==		==Structure of Sortase A from Streptococcus pyogenes with the b7-b8 loop sequence of Enterococcus faecalis Sortase A==
-	<StructureSection load='7s57' size='340' side='right'caption='[[7s57]]' scene=''>	+	<StructureSection load='7s57' size='340' side='right'caption='[[7s57]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7S57 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7S57 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7s57]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7S57 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7S57 FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7s57 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7s57 OCA], [https://pdbe.org/7s57 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7s57 RCSB], [https://www.ebi.ac.uk/pdbsum/7s57 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7s57 ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7s57 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7s57 OCA], [https://pdbe.org/7s57 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7s57 RCSB], [https://www.ebi.ac.uk/pdbsum/7s57 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7s57 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Sequence variation in related proteins is an important characteristic that modulates activity and selectivity. An example of a protein family with a large degree of sequence variation is that of bacterial sortases, which are cysteine transpeptidases on the surface of gram-positive bacteria. Class A sortases are responsible for attachment of diverse proteins to the cell wall to facilitate environmental adaption and interaction. These enzymes are also used in protein engineering applications for sortase-mediated ligations (SML) or sortagging of protein targets. We previously investigated SrtA from Streptococcus pneumoniae, identifying a number of putative beta7-beta8 loop-mediated interactions that affected in vitro enzyme function. We identified residues that contributed to the ability of S. pneumoniae SrtA to recognize several amino acids at the P1' position of the substrate motif, underlined in LPXTG, in contrast to the strict P1' Gly recognition of SrtA from Staphylococcus aureus. However, motivated by the lack of a structural model for the active, monomeric form of S. pneumoniae SrtA, here, we expanded our studies to other Streptococcus SrtA proteins. We solved the first monomeric structure of S. agalactiae SrtA which includes the C-terminus, and three others of beta7-beta8 loop chimeras from S. pyogenes and S. agalactiae SrtA. These structures and accompanying biochemical data support our previously identified beta7-beta8 loop-mediated interactions and provide additional insight into their role in Class A sortase substrate selectivity. A greater understanding of individual SrtA sequence and structural determinants of target selectivity may also facilitate the design or discovery of improved sortagging tools.
		+
		+	Structural and biochemical analyses of selectivity determinants in chimeric Streptococcus Class A sortase enzymes.,Gao M, Johnson DA, Piper IM, Kodama HM, Svendsen JE, Tahti E, Longshore-Neate F, Vogel B, Antos JM, Amacher JF Protein Sci. 2021 Dec 22. doi: 10.1002/pro.4266. PMID:34939250<ref>PMID:34939250</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7s57" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Amacher JF]]	+	[[Category: Amacher, J F]]
-	[[Category: Antos JM]]	+	[[Category: Antos, J M]]
-	[[Category: Gao M]]	+	[[Category: Gao, M]]
-	[[Category: Johnson DA]]	+	[[Category: Johnson, D A]]
-	[[Category: Svendsen JE]]	+	[[Category: Svendsen, J E]]
		+	[[Category: Eight-stranded beta barrel]]
		+	[[Category: Housekeeping sortase]]
		+	[[Category: Hydrolase]]
		+	[[Category: Sortase]]
		+	[[Category: Sortase-fold]]
		+	[[Category: Surface protein]]
		+	[[Category: Transpeptidase]]

OCA at 09:59, 12 January 2022

2022-01-12T09:59:32Z

←Older revision		Revision as of 09:59, 12 January 2022
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7s57 ~~is ON HOLD until Paper Publication~~	+	==Structure of Sortase A from Streptococcus pyogenes with the b7-b8 loop sequence of Enterococcus faecalis Sortase A==
-		+	<StructureSection load='7s57' size='340' side='right'caption='[[7s57]]' scene=''>
-	~~Authors~~: ~~Svendsen, J~~.E., ~~Johnson, D~~.A., ~~Gao~~, M., ~~Antos, J~~.M., ~~Amacher, J~~.F.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7S57 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7S57 FirstGlance]. <br>
-	~~Description: Structure of Sortase A from Streptococcus pyogenes with the b7-b8 loop sequence of Enterococcus faecalis Sortase A~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7s57 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7s57 OCA], [https://pdbe.org/7s57 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7s57 RCSB], [https://www.ebi.ac.uk/pdbsum/7s57 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7s57 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Gao, M~~]]	+	__TOC__
-	[[Category: ~~Svendsen, J.E~~]]	+	</StructureSection>
-	[[Category: ~~Johnson, D.A~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Amacher, J.F~~]]	+	[[Category: Amacher JF]]
-	[[Category: ~~Antos, J.M~~]]	+	[[Category: Antos JM]]
		+	[[Category: Gao M]]
		+	[[Category: Johnson DA]]
		+	[[Category: Svendsen JE]]

OCA at 12:39, 27 October 2021

2021-10-27T12:39:14Z

←Older revision		Revision as of 12:39, 27 October 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7s57 is ON HOLD	+	The entry 7s57 is ON HOLD until Paper Publication

	Authors: Svendsen, J.E., Johnson, D.A., Gao, M., Antos, J.M., Amacher, J.F.		Authors: Svendsen, J.E., Johnson, D.A., Gao, M., Antos, J.M., Amacher, J.F.

OCA: Protected "7s57" [edit=sysop:move=sysop]

2021-09-15T08:22:24Z

Protected "7s57" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:22, 15 September 2021

OCA: New page: '''Unreleased structure''' The entry 7s57 is ON HOLD Authors: Svendsen, J.E., Johnson, D.A., Gao, M., Antos, J.M., Amacher, J.F. Description: Structure of Sortase A from Streptococcus ...

2021-09-15T08:22:23Z

New page: '''Unreleased structure''' The entry 7s57 is ON HOLD Authors: Svendsen, J.E., Johnson, D.A., Gao, M., Antos, J.M., Amacher, J.F. Description: Structure of Sortase A from Streptococcus ...

New page

'''Unreleased structure'''

The entry 7s57 is ON HOLD

Authors: Svendsen, J.E., Johnson, D.A., Gao, M., Antos, J.M., Amacher, J.F.

Description: Structure of Sortase A from Streptococcus pyogenes with the b7-b8 loop sequence of Enterococcus faecalis Sortase A
[[Category: Unreleased Structures]]
[[Category: Gao, M]]
[[Category: Svendsen, J.E]]
[[Category: Johnson, D.A]]
[[Category: Amacher, J.F]]
[[Category: Antos, J.M]]