7s5f - Revision history

OCA at 16:44, 18 October 2023

2023-10-18T16:44:55Z

←Older revision		Revision as of 16:44, 18 October 2023
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	<StructureSection load='7s5f' size='340' side='right'caption='[[7s5f]], [[Resolution\|resolution]] 1.72Å' scene=''>		<StructureSection load='7s5f' size='340' side='right'caption='[[7s5f]], [[Resolution\|resolution]] 1.72Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7s5f]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7S5F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7S5F FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7s5f]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Apium_graveolens Apium graveolens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7S5F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7S5F FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CS2:D-MANNONIC+ACID'>CS2</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.72Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CS2:D-MANNONIC+ACID'>CS2</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7s5f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7s5f OCA], [https://pdbe.org/7s5f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7s5f RCSB], [https://www.ebi.ac.uk/pdbsum/7s5f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7s5f ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7s5f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7s5f OCA], [https://pdbe.org/7s5f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7s5f RCSB], [https://www.ebi.ac.uk/pdbsum/7s5f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7s5f ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A1U9WT24_APIGR A0A1U9WT24_APIGR]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Apium graveolens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Ballicora~~, M A~~]]	+	[[Category: Ballicora MA]]
-	[[Category: Bhayani~~, J A~~]]	+	[[Category: Bhayani JA]]
-	[[Category: Cereijo~~, A E~~]]	+	[[Category: Cereijo AE]]
-	[[Category: Figueroa~~, C M~~]]	+	[[Category: Figueroa CM]]
-	[[Category: Hartman~~, M D~~]]	+	[[Category: Hartman MD]]
-	[[Category: Iglesias~~, A A~~]]	+	[[Category: Iglesias AA]]
-	[[Category: Liu, D]]	+	[[Category: Liu D]]
-	[[Category: Romina~~, I M~~]]	+	[[Category: Romina IM]]
-	[[Category: Zheng, Y]]	+	[[Category: Zheng Y]]
-	~~[[Category: Aldo/keto reductase superfamily]]~~	+
-	~~[[Category: Aldose-6-phosphate reductase]]~~	+
-	~~[[Category: Apium graveolen]]~~	+
-	~~[[Category: Celery]]~~	+
-	~~[[Category: Mannose-6-phosphate reductase]]~~	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Peach]]~~	+
-	~~[[Category: Prunus persica~~]]	+

OCA at 12:00, 23 March 2022

2022-03-23T12:00:42Z

←Older revision		Revision as of 12:00, 23 March 2022
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	==Crystal structure of mannose-6-phosphate reductase from celery (Apium graveolens) leaves with NADP+ and mannonic acid bound==		==Crystal structure of mannose-6-phosphate reductase from celery (Apium graveolens) leaves with NADP+ and mannonic acid bound==
-	<StructureSection load='7s5f' size='340' side='right'caption='[[7s5f]]' scene=''>	+	<StructureSection load='7s5f' size='340' side='right'caption='[[7s5f]], [[Resolution\|resolution]] 1.72Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7S5F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7S5F FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7s5f]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7S5F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7S5F FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7s5f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7s5f OCA], [https://pdbe.org/7s5f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7s5f RCSB], [https://www.ebi.ac.uk/pdbsum/7s5f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7s5f ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CS2:D-MANNONIC+ACID'>CS2</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7s5f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7s5f OCA], [https://pdbe.org/7s5f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7s5f RCSB], [https://www.ebi.ac.uk/pdbsum/7s5f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7s5f ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Sugar-alcohols are major photosynthetic products in plant species from the Apiaceae and Plantaginaceae families. Mannose-6-phosphate reductase (Man6PRase) and aldose-6-phosphate reductase (Ald6PRase) are key enzymes for synthesizing mannitol and glucitol in celery (Apium graveolens) and peach (Prunus persica), respectively. In this work, we report the first crystal structures of dimeric plant aldo/keto reductases, celery Man6PRase (solved in the presence of mannonic acid and NADP+) and peach Ald6PRase (obtained in the apo-form). Both structures displayed the typical TIM barrel folding commonly observed in proteins from the aldo/keto reductase superfamily. Analysis of the Man6PRase holo-form showed that residues putatively involved in the catalytic mechanism are located close to the nicotinamide ring from NADP+, where the hydride transfer to the sugar-phosphate should take place. Additionally, we found that Lys48 is important for the binding of the sugar-phosphate. Interestingly, the Man6PRase K48A mutant had a lower catalytic efficiency with mannose 6-phosphate but higher catalytic efficiency with mannose than the wild type. Overall, our work sheds light on the structure-function relationships of important enzymes to synthesize sugar-alcohols in plants.
		+
		+	Structural Determinants of Sugar-Alcohol Biosynthesis in Plants: The Crystal Structures of Mannose-6-Phosphate and Aldose-6-Phosphate Reductases.,Minen RI, Bhayani JA, Hartman MD, Cereijo AE, Zheng Y, Ballicora MA, Iglesias AA, Liu D, Figueroa CM Plant Cell Physiol. 2022 Mar 3. pii: 6542136. doi: 10.1093/pcp/pcac029. PMID:35243499<ref>PMID:35243499</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7s5f" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Ballicora MA]]	+	[[Category: Ballicora, M A]]
-	[[Category: Bhayani JA]]	+	[[Category: Bhayani, J A]]
-	[[Category: Cereijo AE]]	+	[[Category: Cereijo, A E]]
-	[[Category: Figueroa CM]]	+	[[Category: Figueroa, C M]]
-	[[Category: Hartman MD]]	+	[[Category: Hartman, M D]]
-	[[Category: Iglesias AA]]	+	[[Category: Iglesias, A A]]
-	[[Category: Liu D]]	+	[[Category: Liu, D]]
-	[[Category: Romina IM]]	+	[[Category: Romina, I M]]
-	[[Category: Zheng Y]]	+	[[Category: Zheng, Y]]
		+	[[Category: Aldo/keto reductase superfamily]]
		+	[[Category: Aldose-6-phosphate reductase]]
		+	[[Category: Apium graveolen]]
		+	[[Category: Celery]]
		+	[[Category: Mannose-6-phosphate reductase]]
		+	[[Category: Oxidoreductase]]
		+	[[Category: Peach]]
		+	[[Category: Prunus persica]]

OCA at 06:16, 16 March 2022

2022-03-16T06:16:52Z

←Older revision		Revision as of 06:16, 16 March 2022
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7s5f ~~is ON HOLD until Paper Publication~~	+	==Crystal structure of mannose-6-phosphate reductase from celery (Apium graveolens) leaves with NADP+ and mannonic acid bound==
-		+	<StructureSection load='7s5f' size='340' side='right'caption='[[7s5f]]' scene=''>
-	~~Authors~~: ~~Zheng, Y~~.~~, Bhayani, J~~.A.~~, Romina, I~~.M.~~, Hartman, M~~.D.~~, Cereijo, A~~.E., ~~Ballicora, M~~.A., ~~Iglesias~~, A.A., ~~Figueroa, C~~.M., ~~Liu, D~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7S5F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7S5F FirstGlance]. <br>
-	~~Description: Crystal structure of mannose-6-phosphate reductase from celery (Apium graveolens) leaves with NADP+ and mannonic acid bound~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7s5f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7s5f OCA], [https://pdbe.org/7s5f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7s5f RCSB], [https://www.ebi.ac.uk/pdbsum/7s5f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7s5f ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Zheng, Y~~]]	+	__TOC__
-	[[Category: ~~Romina, I.M~~]]	+	</StructureSection>
-	[[Category: ~~Hartman, M.D~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Iglesias, A.A~~]]	+	[[Category: Ballicora MA]]
-	[[Category: ~~Bhayani, J.A~~]]	+	[[Category: Bhayani JA]]
-	[[Category: ~~Liu, D~~]]	+	[[Category: Cereijo AE]]
-	[[Category: ~~Cereijo, A.E~~]]	+	[[Category: Figueroa CM]]
-	[[Category: ~~Figueroa, C.M~~]]	+	[[Category: Hartman MD]]
-	[[Category: ~~Ballicora, M.A~~]]	+	[[Category: Iglesias AA]]
		+	[[Category: Liu D]]
		+	[[Category: Romina IM]]
		+	[[Category: Zheng Y]]

OCA at 15:03, 20 October 2021

2021-10-20T15:03:32Z

←Older revision		Revision as of 15:03, 20 October 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7s5f is ON HOLD	+	The entry 7s5f is ON HOLD until Paper Publication

	Authors: Zheng, Y., Bhayani, J.A., Romina, I.M., Hartman, M.D., Cereijo, A.E., Ballicora, M.A., Iglesias, A.A., Figueroa, C.M., Liu, D.		Authors: Zheng, Y., Bhayani, J.A., Romina, I.M., Hartman, M.D., Cereijo, A.E., Ballicora, M.A., Iglesias, A.A., Figueroa, C.M., Liu, D.
Line 7:		Line 7:
	Description: Crystal structure of mannose-6-phosphate reductase from celery (Apium graveolens) leaves with NADP+ and mannonic acid bound		Description: Crystal structure of mannose-6-phosphate reductase from celery (Apium graveolens) leaves with NADP+ and mannonic acid bound
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Ballicora, M.A]]
-	[[Category: Liu, D]]
-	[[Category: Iglesias, A.A]]
	[[Category: Zheng, Y]]		[[Category: Zheng, Y]]
	[[Category: Romina, I.M]]		[[Category: Romina, I.M]]
-	[[Category: Cereijo, A.E]]
-	[[Category: Bhayani, J.A]]
	[[Category: Hartman, M.D]]		[[Category: Hartman, M.D]]
		+	[[Category: Iglesias, A.A]]
		+	[[Category: Bhayani, J.A]]
		+	[[Category: Liu, D]]
		+	[[Category: Cereijo, A.E]]
	[[Category: Figueroa, C.M]]		[[Category: Figueroa, C.M]]
		+	[[Category: Ballicora, M.A]]

OCA: Protected "7s5f" [edit=sysop:move=sysop]

2021-09-22T05:37:55Z

Protected "7s5f" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:37, 22 September 2021

OCA: New page: '''Unreleased structure''' The entry 7s5f is ON HOLD Authors: Zheng, Y., Bhayani, J.A., Romina, I.M., Hartman, M.D., Cereijo, A.E., Ballicora, M.A., Iglesias, A.A., Figueroa, C.M., Liu,...

2021-09-22T05:37:54Z

New page: '''Unreleased structure''' The entry 7s5f is ON HOLD Authors: Zheng, Y., Bhayani, J.A., Romina, I.M., Hartman, M.D., Cereijo, A.E., Ballicora, M.A., Iglesias, A.A., Figueroa, C.M., Liu,...

New page

'''Unreleased structure'''

The entry 7s5f is ON HOLD

Authors: Zheng, Y., Bhayani, J.A., Romina, I.M., Hartman, M.D., Cereijo, A.E., Ballicora, M.A., Iglesias, A.A., Figueroa, C.M., Liu, D.

Description: Crystal structure of mannose-6-phosphate reductase from celery (Apium graveolens) leaves with NADP+ and mannonic acid bound
[[Category: Unreleased Structures]]
[[Category: Ballicora, M.A]]
[[Category: Liu, D]]
[[Category: Iglesias, A.A]]
[[Category: Zheng, Y]]
[[Category: Romina, I.M]]
[[Category: Cereijo, A.E]]
[[Category: Bhayani, J.A]]
[[Category: Hartman, M.D]]
[[Category: Figueroa, C.M]]