7sao - Revision history

OCA at 16:48, 18 October 2023

2023-10-18T16:48:27Z

←Older revision		Revision as of 16:48, 18 October 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7sao]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Locusta_migratoria Locusta migratoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SAO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SAO FirstGlance]. <br>		<table><tr><td colspan='2'>[[7sao]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Locusta_migratoria Locusta migratoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SAO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SAO FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.77Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7sao FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7sao OCA], [https://pdbe.org/7sao PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7sao RCSB], [https://www.ebi.ac.uk/pdbsum/7sao PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7sao ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7sao FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7sao OCA], [https://pdbe.org/7sao PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7sao RCSB], [https://www.ebi.ac.uk/pdbsum/7sao PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7sao ProSAT]</span></td></tr>
	</table>		</table>

OCA at 03:16, 8 September 2022

2022-09-08T03:16:08Z

←Older revision		Revision as of 03:16, 8 September 2022
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	==The CTI-homolog pacifastin==		==The CTI-homolog pacifastin==
-	<StructureSection load='7sao' size='340' side='right'caption='[[7sao]]' scene=''>	+	<StructureSection load='7sao' size='340' side='right'caption='[[7sao]], [[Resolution\|resolution]] 1.77Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SAO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SAO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7sao]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Locusta_migratoria Locusta migratoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SAO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SAO FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7sao FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7sao OCA], [https://pdbe.org/7sao PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7sao RCSB], [https://www.ebi.ac.uk/pdbsum/7sao PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7sao ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7sao FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7sao OCA], [https://pdbe.org/7sao PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7sao RCSB], [https://www.ebi.ac.uk/pdbsum/7sao PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7sao ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Cystine-dense peptides (CDPs) are a miniprotein class that can drug difficult targets with high affinity and low immunogenicity. Tools for their design, however, are not as developed as those for small-molecule and antibody drugs. CDPs have diverse taxonomic origins, but structural characterization is lacking. Here, we adapted Iterative Threading ASSEmbly Refinement (I-TASSER) and Rosetta protein modeling software for structural prediction of 4298 CDP scaffolds and performed in silico prescreening for CDP binders to targets of interest. Mammalian display screening of a library of docking-enriched, methionine and tyrosine scanned (DEMYS) CDPs against PD-L1 yielded binders from four distinct CDP scaffolds. One was affinity-matured, and cocrystallography yielded a high-affinity (KD = 202 pM) PD-L1-binding CDP that competes with PD-1 for PD-L1 binding. Its subsequent incorporation into a CD3-binding bispecific T cell engager produced a molecule with pM-range in vitro T cell killing potency and which substantially extends survival in two different xenograft tumor-bearing mouse models. Both in vitro and in vivo, the CDP-incorporating bispecific molecule outperformed a comparator antibody-based molecule. This CDP modeling and DEMYS technique can accelerate CDP therapeutic development.
		+
		+	Ex silico engineering of cystine-dense peptides yielding a potent bispecific T cell engager.,Crook ZR, Girard EJ, Sevilla GP, Brusniak MY, Rupert PB, Friend DJ, Gewe MM, Clarke M, Lin I, Ruff R, Pakiam F, Phi TD, Bandaranayake A, Correnti CE, Mhyre AJ, Nairn NW, Strong RK, Olson JM Sci Transl Med. 2022 May 18;14(645):eabn0402. doi: 10.1126/scitranslmed.abn0402. , Epub 2022 May 18. PMID:35584229<ref>PMID:35584229</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7sao" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Locusta migratoria]]
	[[Category: Gewe MM]]		[[Category: Gewe MM]]
	[[Category: Strong RK]]		[[Category: Strong RK]]

OCA at 04:53, 3 August 2022

2022-08-03T04:53:06Z

←Older revision		Revision as of 04:53, 3 August 2022
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-	'''Unreleased structure'''

-	The ~~entry~~ 7sao ~~is ON HOLD~~	+	==The CTI-homolog pacifastin==
-		+	<StructureSection load='7sao' size='340' side='right'caption='[[7sao]]' scene=''>
-	~~Authors~~: ~~Gewe~~, M.M., ~~Strong~~, R.K.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SAO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SAO FirstGlance]. <br>
-	~~Description~~: ~~The CTI~~-~~homolog pacifastin~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7sao FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7sao OCA], [https://pdbe.org/7sao PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7sao RCSB], [https://www.ebi.ac.uk/pdbsum/7sao PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7sao ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Strong, R.K~~]]	+	__TOC__
-	[[Category: ~~Gewe, M.M~~]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Gewe MM]]
		+	[[Category: Strong RK]]

OCA: Protected "7sao" [edit=sysop:move=sysop]

2021-09-29T07:50:49Z

Protected "7sao" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:50, 29 September 2021

OCA: New page: '''Unreleased structure''' The entry 7sao is ON HOLD Authors: Gewe, M.M., Strong, R.K. Description: The CTI-homolog pacifastin Category: Unreleased Structures [[Category: Strong, R...

2021-09-29T07:50:48Z

New page: '''Unreleased structure''' The entry 7sao is ON HOLD Authors: Gewe, M.M., Strong, R.K. Description: The CTI-homolog pacifastin Category: Unreleased Structures [[Category: Strong, R...

New page

'''Unreleased structure'''

The entry 7sao is ON HOLD

Authors: Gewe, M.M., Strong, R.K.

Description: The CTI-homolog pacifastin
[[Category: Unreleased Structures]]
[[Category: Strong, R.K]]
[[Category: Gewe, M.M]]