7sfa - Revision history

OCA at 16:50, 18 October 2023

2023-10-18T16:50:29Z

←Older revision		Revision as of 16:50, 18 October 2023
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	==Branchiostoma floridae fluorescent protein LanFP10A2==		==Branchiostoma floridae fluorescent protein LanFP10A2==
-	<StructureSection load='7sfa' size='340' side='right'caption='[[7sfa]]' scene=''>	+	<StructureSection load='7sfa' size='340' side='right'caption='[[7sfa]], [[Resolution\|resolution]] 1.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SFA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SFA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7sfa]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Branchiostoma_floridae Branchiostoma floridae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SFA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SFA FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7sfa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7sfa OCA], [https://pdbe.org/7sfa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7sfa RCSB], [https://www.ebi.ac.uk/pdbsum/7sfa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7sfa ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.65Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=JBY:(2S)-2-{(4Z)-2-(aminomethyl)-4-[(4-hydroxyphenyl)methylidene]-5-oxo-4,5-dihydro-1H-imidazol-1-yl}propanoic+acid'>JBY</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7sfa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7sfa OCA], [https://pdbe.org/7sfa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7sfa RCSB], [https://www.ebi.ac.uk/pdbsum/7sfa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7sfa ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/C3YRA2_BRAFL C3YRA2_BRAFL]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	We describe an engineered violet fluorescent protein from the lancelet Branchiostoma floridae (bfVFP). This is the first example of a GFP-like fluorescent protein with a stable fluorescent chromophore lacking an imidazolinone ring; instead, it consists of oxidized tyrosine 68 flanked by glycine 67 and alanine 69. bfVFP contains the simplest chromophore reported in fluorescent proteins and was generated from the yellow protein lanFP10A2 by two synergetic mutations, S148H and C166I. The chromophore structure was confirmed crystallographically and by high-resolution mass spectrometry. The photophysical characteristics of bfVFP (323/430 nm, quantum yield 0.33, and Ec 14,300 M(-1) cm(-1) ) make it potentially useful for multicolor experiments to expand the excitation range of available FP biomarkers and Forster resonance energy transfer with blue and cyan fluorescent protein acceptors.
		+
		+	A novel violet fluorescent protein contains a unique oxidized tyrosine as the simplest chromophore ever reported in fluorescent proteins.,Roldan-Salgado A, Muslinkina L, Pletnev S, Pletneva N, Pletnev V, Gaytan P Protein Sci. 2022 Mar;31(3):688-700. doi: 10.1002/pro.4265. Epub 2022 Jan 3. PMID:34936154<ref>PMID:34936154</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7sfa" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Branchiostoma floridae]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Muslinkina L]]		[[Category: Muslinkina L]]

OCA at 10:46, 30 March 2022

2022-03-30T10:46:50Z

←Older revision		Revision as of 10:46, 30 March 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7sfa ~~is ON HOLD until Paper Publication~~	+	==Branchiostoma floridae fluorescent protein LanFP10A2==
-		+	<StructureSection load='7sfa' size='340' side='right'caption='[[7sfa]]' scene=''>
-	~~Authors~~: ~~Pletnev~~, S., ~~Pletneva~~, N., ~~Pletnev, V~~.Z., ~~Muslinkina, L~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SFA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SFA FirstGlance]. <br>
-	~~Description: Branchiostoma floridae fluorescent protein LanFP10A2~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7sfa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7sfa OCA], [https://pdbe.org/7sfa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7sfa RCSB], [https://www.ebi.ac.uk/pdbsum/7sfa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7sfa ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Pletnev, V.Z~~]]	+	__TOC__
-	[[Category: Pletnev, S]]	+	</StructureSection>
-	[[Category: ~~Pletneva, N~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Muslinkina, L~~]]	+	[[Category: Muslinkina L]]
		+	[[Category: Pletnev S]]
		+	[[Category: Pletnev VZ]]
		+	[[Category: Pletneva N]]

OCA at 12:43, 27 October 2021

2021-10-27T12:43:47Z

←Older revision		Revision as of 12:43, 27 October 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7sfa is ON HOLD	+	The entry 7sfa is ON HOLD until Paper Publication

	Authors: Pletnev, S., Pletneva, N., Pletnev, V.Z., Muslinkina, L.		Authors: Pletnev, S., Pletneva, N., Pletnev, V.Z., Muslinkina, L.

OCA: Protected "7sfa" [edit=sysop:move=sysop]

2021-10-13T10:26:32Z

Protected "7sfa" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:26, 13 October 2021

OCA: New page: '''Unreleased structure''' The entry 7sfa is ON HOLD Authors: Pletnev, S., Pletneva, N., Pletnev, V.Z., Muslinkina, L. Description: Branchiostoma floridae fluorescent protein LanFP10A2...

2021-10-13T10:26:30Z

New page: '''Unreleased structure''' The entry 7sfa is ON HOLD Authors: Pletnev, S., Pletneva, N., Pletnev, V.Z., Muslinkina, L. Description: Branchiostoma floridae fluorescent protein LanFP10A2...

New page

'''Unreleased structure'''

The entry 7sfa is ON HOLD

Authors: Pletnev, S., Pletneva, N., Pletnev, V.Z., Muslinkina, L.

Description: Branchiostoma floridae fluorescent protein LanFP10A2
[[Category: Unreleased Structures]]
[[Category: Pletnev, V.Z]]
[[Category: Pletnev, S]]
[[Category: Pletneva, N]]
[[Category: Muslinkina, L]]