7sqr - Revision history

OCA at 04:15, 8 September 2022

2022-09-08T04:15:32Z

←Older revision		Revision as of 04:15, 8 September 2022
Line 1:		Line 1:

	==201phi2-1 Chimallin localized tetramer reconstruction==		==201phi2-1 Chimallin localized tetramer reconstruction==
-	<StructureSection load='7sqr' size='340' side='right'caption='[[7sqr]]' scene=''>	+	<StructureSection load='7sqr' size='340' side='right'caption='[[7sqr]], [[Resolution\|resolution]] 3.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SQR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SQR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7sqr]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_phage_201phi2-1 Pseudomonas phage 201phi2-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SQR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SQR FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7sqr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7sqr OCA], [https://pdbe.org/7sqr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7sqr RCSB], [https://www.ebi.ac.uk/pdbsum/7sqr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7sqr ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7sqr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7sqr OCA], [https://pdbe.org/7sqr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7sqr RCSB], [https://www.ebi.ac.uk/pdbsum/7sqr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7sqr ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/GP105_BP201 GP105_BP201]] Forms a proteinaceous shell that encloses the viral DNA and compartmentalizes proteins and DNA during viral infection (PubMed:28813669, PubMed:28082593). This shell is the site of viral replication, with the proteins involved in DNA replication localized inside the compartment (PubMed:28813669, PubMed:28082593). Provides a surface for docking of capsids during packaging (PubMed:28082593).<ref>PMID:28082593</ref> <ref>PMID:28813669</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Bacteria encode myriad defences that target the genomes of infecting bacteriophage, including restriction-modification and CRISPR-Cas systems(1). In response, one family of large bacteriophages uses a nucleus-like compartment to protect its replicating genomes by excluding host defence factors(2-4). However, the principal composition and structure of this compartment remain unknown. Here we find that the bacteriophage nuclear shell assembles primarily from one protein, which we name chimallin (ChmA). Combining cryo-electron tomography of nuclear shells in bacteriophage-infected cells and cryo-electron microscopy of a minimal chimallin compartment in vitro, we show that chimallin self-assembles as a flexible sheet into closed micrometre-scale compartments. The architecture and assembly dynamics of the chimallin shell suggest mechanisms for its nucleation and growth, and its role as a scaffold for phage-encoded factors mediating macromolecular transport, cytoskeletal interactions, and viral maturation.
		+
		+	Architecture and self-assembly of the jumbo bacteriophage nuclear shell.,Laughlin TG, Deep A, Prichard AM, Seitz C, Gu Y, Enustun E, Suslov S, Khanna K, Birkholz EA, Armbruster E, McCammon JA, Amaro RE, Pogliano J, Corbett KD, Villa E Nature. 2022 Aug;608(7922):429-435. doi: 10.1038/s41586-022-05013-4. Epub 2022, Aug 3. PMID:35922510<ref>PMID:35922510</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7sqr" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Pseudomonas phage 201phi2-1]]
	[[Category: Amaro RE]]		[[Category: Amaro RE]]
	[[Category: Birkholz EA]]		[[Category: Birkholz EA]]

OCA at 11:07, 31 August 2022

2022-08-31T11:07:58Z

←Older revision		Revision as of 11:07, 31 August 2022
Line 1:		Line 1:

	==201phi2-1 Chimallin localized tetramer reconstruction==		==201phi2-1 Chimallin localized tetramer reconstruction==
-	<StructureSection load='7sqr' size='340' side='right'caption='[[7sqr]]~~, [[Resolution\|resolution]] 3.40Å~~' scene=''>	+	<StructureSection load='7sqr' size='340' side='right'caption='[[7sqr]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>~~[[7sqr]] is a 12 chain structure.~~ Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SQR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SQR FirstGlance]. <br>	+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SQR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SQR FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7sqr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7sqr OCA], [https://pdbe.org/7sqr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7sqr RCSB], [https://www.ebi.ac.uk/pdbsum/7sqr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7sqr ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7sqr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7sqr OCA], [https://pdbe.org/7sqr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7sqr RCSB], [https://www.ebi.ac.uk/pdbsum/7sqr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7sqr ProSAT]</span></td></tr>
	</table>		</table>
-	== Function ==
-	[[https://www.uniprot.org/uniprot/GP105_BP201 GP105_BP201]] Forms a proteinaceous shell that encloses the viral DNA and compartmentalizes proteins and DNA during viral infection (PubMed:28813669, PubMed:28082593). This shell is the site of viral replication, with the proteins involved in DNA replication localized inside the compartment (PubMed:28813669, PubMed:28082593). Provides a surface for docking of capsids during packaging (PubMed:28082593).<ref>PMID:28082593</ref> <ref>PMID:28813669</ref>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Bacteria encode myriad defences that target the genomes of infecting bacteriophage, including restriction-modification and CRISPR-Cas systems(1). In response, one family of large bacteriophages uses a nucleus-like compartment to protect its replicating genomes by excluding host defence factors(2-4). However, the principal composition and structure of this compartment remain unknown. Here we find that the bacteriophage nuclear shell assembles primarily from one protein, which we name chimallin (ChmA). Combining cryo-electron tomography of nuclear shells in bacteriophage-infected cells and cryo-electron microscopy of a minimal chimallin compartment in vitro, we show that chimallin self-assembles as a flexible sheet into closed micrometre-scale compartments. The architecture and assembly dynamics of the chimallin shell suggest mechanisms for its nucleation and growth, and its role as a scaffold for phage-encoded factors mediating macromolecular transport, cytoskeletal interactions, and viral maturation.
-
-	Architecture and self-assembly of the jumbo bacteriophage nuclear shell.,Laughlin TG, Deep A, Prichard AM, Seitz C, Gu Y, Enustun E, Suslov S, Khanna K, Birkholz EA, Armbruster E, McCammon JA, Amaro RE, Pogliano J, Corbett KD, Villa E Nature. 2022 Aug;608(7922):429-435. doi: 10.1038/s41586-022-05013-4. Epub 2022, Aug 3. PMID:35922510<ref>PMID:35922510</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 7sqr" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Amaro~~, R E~~]]	+	[[Category: Amaro RE]]
-	[[Category: Birkholz~~, E A~~]]	+	[[Category: Birkholz EA]]
-	[[Category: Corbett~~, K D~~]]	+	[[Category: Corbett KD]]
-	[[Category: Deep, A]]	+	[[Category: Deep A]]
-	[[Category: Enustun, E]]	+	[[Category: Enustun E]]
-	[[Category: Gu, Y]]	+	[[Category: Gu Y]]
-	[[Category: Khanna, K]]	+	[[Category: Khanna K]]
-	[[Category: Laughlin~~, T G~~]]	+	[[Category: Laughlin TG]]
-	[[Category: Pogliano, J]]	+	[[Category: Pogliano J]]
-	[[Category: Prichard~~, A M~~]]	+	[[Category: Prichard AM]]
-	[[Category: Seitz, C]]	+	[[Category: Seitz C]]
-	[[Category: Suslov, S]]	+	[[Category: Suslov S]]
-	[[Category: Villa, E]]	+	[[Category: Villa E]]
-	~~[[Category: Phage]]~~	+
-	~~[[Category: Structural protein]]~~	+
-	~~[[Category: Viral protein~~]]	+

OCA at 06:40, 18 August 2022

2022-08-18T06:40:33Z

←Older revision		Revision as of 06:40, 18 August 2022
Line 1:		Line 1:

	==201phi2-1 Chimallin localized tetramer reconstruction==		==201phi2-1 Chimallin localized tetramer reconstruction==
-	<StructureSection load='7sqr' size='340' side='right'caption='[[7sqr]]' scene=''>	+	<StructureSection load='7sqr' size='340' side='right'caption='[[7sqr]], [[Resolution\|resolution]] 3.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SQR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SQR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7sqr]] is a 12 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SQR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SQR FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7sqr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7sqr OCA], [https://pdbe.org/7sqr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7sqr RCSB], [https://www.ebi.ac.uk/pdbsum/7sqr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7sqr ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7sqr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7sqr OCA], [https://pdbe.org/7sqr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7sqr RCSB], [https://www.ebi.ac.uk/pdbsum/7sqr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7sqr ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/GP105_BP201 GP105_BP201]] Forms a proteinaceous shell that encloses the viral DNA and compartmentalizes proteins and DNA during viral infection (PubMed:28813669, PubMed:28082593). This shell is the site of viral replication, with the proteins involved in DNA replication localized inside the compartment (PubMed:28813669, PubMed:28082593). Provides a surface for docking of capsids during packaging (PubMed:28082593).<ref>PMID:28082593</ref> <ref>PMID:28813669</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Bacteria encode myriad defences that target the genomes of infecting bacteriophage, including restriction-modification and CRISPR-Cas systems(1). In response, one family of large bacteriophages uses a nucleus-like compartment to protect its replicating genomes by excluding host defence factors(2-4). However, the principal composition and structure of this compartment remain unknown. Here we find that the bacteriophage nuclear shell assembles primarily from one protein, which we name chimallin (ChmA). Combining cryo-electron tomography of nuclear shells in bacteriophage-infected cells and cryo-electron microscopy of a minimal chimallin compartment in vitro, we show that chimallin self-assembles as a flexible sheet into closed micrometre-scale compartments. The architecture and assembly dynamics of the chimallin shell suggest mechanisms for its nucleation and growth, and its role as a scaffold for phage-encoded factors mediating macromolecular transport, cytoskeletal interactions, and viral maturation.
		+
		+	Architecture and self-assembly of the jumbo bacteriophage nuclear shell.,Laughlin TG, Deep A, Prichard AM, Seitz C, Gu Y, Enustun E, Suslov S, Khanna K, Birkholz EA, Armbruster E, McCammon JA, Amaro RE, Pogliano J, Corbett KD, Villa E Nature. 2022 Aug;608(7922):429-435. doi: 10.1038/s41586-022-05013-4. Epub 2022, Aug 3. PMID:35922510<ref>PMID:35922510</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7sqr" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Amaro RE]]	+	[[Category: Amaro, R E]]
-	[[Category: Birkholz EA]]	+	[[Category: Birkholz, E A]]
-	[[Category: Corbett KD]]	+	[[Category: Corbett, K D]]
-	[[Category: Deep A]]	+	[[Category: Deep, A]]
-	[[Category: Enustun E]]	+	[[Category: Enustun, E]]
-	[[Category: Gu Y]]	+	[[Category: Gu, Y]]
-	[[Category: Khanna K]]	+	[[Category: Khanna, K]]
-	[[Category: Laughlin TG]]	+	[[Category: Laughlin, T G]]
-	[[Category: Pogliano J]]	+	[[Category: Pogliano, J]]
-	[[Category: Prichard AM]]	+	[[Category: Prichard, A M]]
-	[[Category: Seitz C]]	+	[[Category: Seitz, C]]
-	[[Category: Suslov S]]	+	[[Category: Suslov, S]]
-	[[Category: Villa E]]	+	[[Category: Villa, E]]
		+	[[Category: Phage]]
		+	[[Category: Structural protein]]
		+	[[Category: Viral protein]]

OCA at 18:04, 27 July 2022

2022-07-27T18:04:07Z

←Older revision		Revision as of 18:04, 27 July 2022
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7sqr is ~~ON HOLD until Paper Publication~~	+	==201phi2-1 Chimallin localized tetramer reconstruction==
-		+	<StructureSection load='7sqr' size='340' side='right'caption='[[7sqr]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SQR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SQR FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7sqr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7sqr OCA], [https://pdbe.org/7sqr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7sqr RCSB], [https://www.ebi.ac.uk/pdbsum/7sqr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7sqr ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Amaro RE]]
		+	[[Category: Birkholz EA]]
		+	[[Category: Corbett KD]]
		+	[[Category: Deep A]]
		+	[[Category: Enustun E]]
		+	[[Category: Gu Y]]
		+	[[Category: Khanna K]]
		+	[[Category: Laughlin TG]]
		+	[[Category: Pogliano J]]
		+	[[Category: Prichard AM]]
		+	[[Category: Seitz C]]
		+	[[Category: Suslov S]]
		+	[[Category: Villa E]]

OCA at 15:38, 15 December 2021

2021-12-15T15:38:30Z

←Older revision		Revision as of 15:38, 15 December 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7sqr is ON HOLD	+	The entry 7sqr is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7sqr" [edit=sysop:move=sysop]

2021-11-17T12:13:30Z

Protected "7sqr" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:13, 17 November 2021

OCA: New page: '''Unreleased structure''' The entry 7sqr is ON HOLD Authors: Description: Category: Unreleased Structures

2021-11-17T12:13:29Z

New page: '''Unreleased structure''' The entry 7sqr is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7sqr is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]