7swj - Revision history

OCA at 11:20, 14 June 2023

2023-06-14T11:20:53Z

←Older revision		Revision as of 11:20, 14 June 2023
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	==KirBac1.1 mutant - I131C==		==KirBac1.1 mutant - I131C==
-	<StructureSection load='7swj' size='340' side='right'caption='[[7swj~~]], [[NMR_Ensembles_of_Models \| 10 NMR models~~]]' scene=''>	+	<StructureSection load='7swj' size='340' side='right'caption='[[7swj]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7swj]] is a 4 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SWJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SWJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7swj]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Burkholderia_pseudomallei Burkholderia pseudomallei]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SWJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SWJ FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7swj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7swj OCA], [https://pdbe.org/7swj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7swj RCSB], [https://www.ebi.ac.uk/pdbsum/7swj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7swj ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7swj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7swj OCA], [https://pdbe.org/7swj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7swj RCSB], [https://www.ebi.ac.uk/pdbsum/7swj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7swj ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/P83698_BURPE P83698_BURPE]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 19:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Burkholderia pseudomallei]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Amani, R]]	+	[[Category: Amani R]]
-	[[Category: Wylie~~, B J]]~~	+	[[Category: Wylie BJ]]
-	~~[[Category: Membrane protein~~]]	+

OCA at 08:45, 23 February 2022

2022-02-23T08:45:57Z

←Older revision		Revision as of 08:45, 23 February 2022
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	==KirBac1.1 mutant - I131C==		==KirBac1.1 mutant - I131C==
-	<StructureSection load='7swj' size='340' side='right'caption='[[7swj]]' scene=''>	+	<StructureSection load='7swj' size='340' side='right'caption='[[7swj]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full ~~crystallographic~~ information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SWJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SWJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7swj]] is a 4 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SWJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SWJ FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7swj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7swj OCA], [https://pdbe.org/7swj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7swj RCSB], [https://www.ebi.ac.uk/pdbsum/7swj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7swj ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7swj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7swj OCA], [https://pdbe.org/7swj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7swj RCSB], [https://www.ebi.ac.uk/pdbsum/7swj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7swj ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	NMR structures of membrane proteins are often hampered by poor chemical shift dispersion and internal dynamics which limit resolved distance restraints. However, the ordering and topology of these systems can be defined with site-specific water or lipid proximity. Membrane protein water accessibility surface area is often investigated as a topological function via solid-state NMR. Here we leverage water-edited solid-state NMR measurements in simulated annealing calculations to refine a membrane protein structure. This is demonstrated on the inward rectifier K(+) channel KirBac1.1 found in Burkholderia pseudomallei. KirBac1.1 is homologous to human Kir channels, sharing a nearly identical fold. Like many existing Kir channel crystal structures, the 1p7b crystal structure is incomplete, missing 85 out of 333 residues, including the N-terminus and C-terminus. We measure solid-state NMR water proximity information and use this for refinement of KirBac1.1 using the Xplor-NIH structure determination program. Along with predicted dihedral angles and sparse intra- and inter-subunit distances, we refined the residues 1-300 to atomic resolution. All structural quality metrics indicate these restraints are a powerful way forward to solve high quality structures of membrane proteins using NMR.
		+
		+	Water Accessibility Refinement of the Extended Structure of KirBac1.1 in the Closed State.,Amani R, Schwieters CD, Borcik CG, Eason IR, Han R, Harding BD, Wylie BJ Front Mol Biosci. 2021 Nov 30;8:772855. doi: 10.3389/fmolb.2021.772855., eCollection 2021. PMID:34917650<ref>PMID:34917650</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7swj" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Amani R]]	+	[[Category: Amani, R]]
-	[[Category: Wylie JW]]	+	[[Category: Wylie, B J]]
		+	[[Category: Membrane protein]]

OCA at 07:03, 2 February 2022

2022-02-02T07:03:53Z

←Older revision		Revision as of 07:03, 2 February 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7swj ~~is ON HOLD~~	+	==KirBac1.1 mutant - I131C==
-		+	<StructureSection load='7swj' size='340' side='right'caption='[[7swj]]' scene=''>
-	~~Authors~~: ~~Amani~~, R., ~~Wylie~~, J.W.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7SWJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7SWJ FirstGlance]. <br>
-	~~Description~~: ~~KirBac1~~.~~1 mutant~~ -~~I131C~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7swj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7swj OCA], [https://pdbe.org/7swj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7swj RCSB], [https://www.ebi.ac.uk/pdbsum/7swj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7swj ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: Amani, R]]	+	__TOC__
-	[[Category: Wylie~~, J.W~~]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Amani R]]
		+	[[Category: Wylie JW]]

OCA at 14:02, 22 December 2021

2021-12-22T14:02:33Z

←Older revision		Revision as of 14:02, 22 December 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7swj is ON HOLD ~~until sometime in the future~~	+	The entry 7swj is ON HOLD

	Authors: Amani, R., Wylie, J.W.		Authors: Amani, R., Wylie, J.W.

OCA at 08:34, 8 December 2021

2021-12-08T08:34:27Z

←Older revision		Revision as of 08:34, 8 December 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7swj is ON HOLD	+	The entry 7swj is ON HOLD until sometime in the future

	Authors: Amani, R., Wylie, J.W.		Authors: Amani, R., Wylie, J.W.

OCA: Protected "7swj" [edit=sysop:move=sysop]

2021-12-01T05:12:33Z

Protected "7swj" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:12, 1 December 2021

OCA: New page: '''Unreleased structure''' The entry 7swj is ON HOLD Authors: Amani, R., Wylie, J.W. Description: KirBac1.1 mutant -I131C Category: Unreleased Structures Category: Amani, R [[C...

2021-12-01T05:12:32Z

New page: '''Unreleased structure''' The entry 7swj is ON HOLD Authors: Amani, R., Wylie, J.W. Description: KirBac1.1 mutant -I131C Category: Unreleased Structures Category: Amani, R [[C...

New page

'''Unreleased structure'''

The entry 7swj is ON HOLD

Authors: Amani, R., Wylie, J.W.

Description: KirBac1.1 mutant -I131C
[[Category: Unreleased Structures]]
[[Category: Amani, R]]
[[Category: Wylie, J.W]]