7t68 - Revision history

OCA at 17:00, 18 October 2023

2023-10-18T17:00:54Z

←Older revision		Revision as of 17:00, 18 October 2023
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	<StructureSection load='7t68' size='340' side='right'caption='[[7t68]], [[Resolution\|resolution]] 2.32Å' scene=''>		<StructureSection load='7t68' size='340' side='right'caption='[[7t68]], [[Resolution\|resolution]] 2.32Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7t68]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7T68 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7T68 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7t68]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Chaetomium_thermophilum_var._thermophilum_DSM_1495 Chaetomium thermophilum var. thermophilum DSM 1495]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7T68 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7T68 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BTB:2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>BTB</scene>, <scene name='pdbligand=GII:(2R,3R,4R,5S)-1-[6-(4-azido-2-nitroanilino)hexyl]-2-(hydroxymethyl)piperidine-3,4,5-triol'>GII</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.32Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BTB:2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>BTB</scene>, <scene name='pdbligand=GII:(2R,3R,4R,5S)-1-[6-(4-azido-2-nitroanilino)hexyl]-2-(hydroxymethyl)piperidine-3,4,5-triol'>GII</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7t68 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7t68 OCA], [https://pdbe.org/7t68 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7t68 RCSB], [https://www.ebi.ac.uk/pdbsum/7t68 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7t68 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7t68 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7t68 OCA], [https://pdbe.org/7t68 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7t68 RCSB], [https://www.ebi.ac.uk/pdbsum/7t68 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7t68 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/G0SFD1_CHATD G0SFD1_CHATD]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 7t68" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 7t68" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Alpha-glucosidase 3D structures\|Alpha-glucosidase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Chaetomium thermophilum var. thermophilum DSM 1495]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Karade~~, S S~~]]	+	[[Category: Karade SS]]
-	[[Category: Mariuzza~~, R A]]~~	+	[[Category: Mariuzza RA]]
-	~~[[Category: Alpha glucosidase i]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Hydrolase-inhibitor complex]]~~	+
-	~~[[Category: Inhibitor complex~~]]	+

OCA at 08:16, 25 May 2022

2022-05-25T08:16:42Z

←Older revision		Revision as of 08:16, 25 May 2022
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	==Co-crystal structure of Chaetomium glucosidase with compound UV-5==		==Co-crystal structure of Chaetomium glucosidase with compound UV-5==
-	<StructureSection load='7t68' size='340' side='right'caption='[[7t68]]' scene=''>	+	<StructureSection load='7t68' size='340' side='right'caption='[[7t68]], [[Resolution\|resolution]] 2.32Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7T68 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7T68 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7t68]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7T68 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7T68 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7t68 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7t68 OCA], [https://pdbe.org/7t68 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7t68 RCSB], [https://www.ebi.ac.uk/pdbsum/7t68 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7t68 ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BTB:2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>BTB</scene>, <scene name='pdbligand=GII:(2R,3R,4R,5S)-1-[6-(4-azido-2-nitroanilino)hexyl]-2-(hydroxymethyl)piperidine-3,4,5-triol'>GII</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7t68 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7t68 OCA], [https://pdbe.org/7t68 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7t68 RCSB], [https://www.ebi.ac.uk/pdbsum/7t68 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7t68 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	All viruses depend on host cell proteins for replication. Denying viruses' access to the function of critical host proteins can result in antiviral activity against multiple virus families. In particular, small-molecule drug candidates which inhibit the alpha-glucosidase enzymes of the endoplasmic reticulum (ER) translation quality control (QC) pathway have demonstrated broad-spectrum antiviral activities and low risk for development of viral resistance. However, antiviral drug discovery focused on the ERQC enzyme alpha-glucosidase I (alpha-GluI) has been hampered by difficulties in obtaining crystal structures of complexes with inhibitors. We report here the identification of an orthologous enzyme from a thermophilic fungus, Chaetomium thermophilum (Ct), as a robust surrogate for mammalian ER alpha-GluI and a platform for inhibitor design. Previously annotated only as a hypothetical protein, the Ct protein was validated as a bona fide alpha-glucosidase by comparing its crystal structure to that of mammalian alpha-GluI, by demonstrating enzymatic activity on the unusual alpha-d-Glcp-(1 --> 2)-alpha-d-Glcp-(1 --> 3) substrate glycan, and by showing that well-known inhibitors of mammalian alpha-GluI (1-DNJ, UV-4, UV-5) also inhibit Ct alpha-GluI. Crystal structures of Ct alpha-GluI in complex with three such inhibitors (UV-4, UV-5, EB-0159) revealed extensive interactions with all four enzyme subsites and provided insights into the catalytic mechanism. Identification of ER Ct alpha-GluI as a surrogate for mammalian alpha-GluI will accelerate the structure-guided discovery of broad-spectrum antivirals. This study also highlights Ct as a source of thermostable eukaryotic proteins, such as ER alpha-Glu I, that lack orthologs in bacterial or archaeal thermophiles.
		+
		+	Identification of Endoplasmic Reticulum alpha-Glucosidase I from a Thermophilic Fungus as a Platform for Structure-Guided Antiviral Drug Design.,Karade SS, Kolesnikov A, Treston AM, Mariuzza RA Biochemistry. 2022 Apr 27. doi: 10.1021/acs.biochem.2c00092. PMID:35476408<ref>PMID:35476408</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7t68" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Karade SS]]	+	[[Category: Karade, S S]]
-	[[Category: Mariuzza RA]]	+	[[Category: Mariuzza, R A]]
		+	[[Category: Alpha glucosidase i]]
		+	[[Category: Hydrolase]]
		+	[[Category: Hydrolase-inhibitor complex]]
		+	[[Category: Inhibitor complex]]

OCA at 12:50, 4 May 2022

2022-05-04T12:50:50Z

←Older revision		Revision as of 12:50, 4 May 2022
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7t68 ~~is ON HOLD until Paper Publication~~	+	==Co-crystal structure of Chaetomium glucosidase with compound UV-5==
-		+	<StructureSection load='7t68' size='340' side='right'caption='[[7t68]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7T68 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7T68 FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7t68 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7t68 OCA], [https://pdbe.org/7t68 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7t68 RCSB], [https://www.ebi.ac.uk/pdbsum/7t68 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7t68 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Karade SS]]
		+	[[Category: Mariuzza RA]]

OCA at 05:26, 27 January 2022

2022-01-27T05:26:27Z

←Older revision		Revision as of 05:26, 27 January 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7t68 is ON HOLD	+	The entry 7t68 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7t68" [edit=sysop:move=sysop]

2021-12-22T14:06:31Z

Protected "7t68" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:06, 22 December 2021

OCA: New page: '''Unreleased structure''' The entry 7t68 is ON HOLD Authors: Description: Category: Unreleased Structures

2021-12-22T14:06:30Z

New page: '''Unreleased structure''' The entry 7t68 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7t68 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]