7taa - Revision history

OCA at 02:25, 8 February 2016

2016-02-08T02:25:30Z

←Older revision		Revision as of 02:25, 8 February 2016
Line 2:		Line 2:
	<StructureSection load='7taa' size='340' side='right' caption='[[7taa]], [[Resolution\|resolution]] 1.98Å' scene=''>		<StructureSection load='7taa' size='340' side='right' caption='[[7taa]], [[Resolution\|resolution]] 1.98Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7taa]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Aspergillus_oryzae Aspergillus oryzae~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7TAA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=7TAA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7taa]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aspoz Aspoz]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7TAA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=7TAA FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ABC:MODIFIED+ACARBOSE+HEXASACCHARIDE'>ABC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ABC:MODIFIED+ACARBOSE+HEXASACCHARIDE'>ABC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alpha-amylase Alpha-amylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.1 3.2.1.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alpha-amylase Alpha-amylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.1 3.2.1.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=7taa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7taa OCA], [http://www.rcsb.org/pdb/explore.do?structureId=7taa RCSB], [http://www.ebi.ac.uk/pdbsum/7taa PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=7taa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7taa OCA], [http://pdbe.org/7taa PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=7taa RCSB], [http://www.ebi.ac.uk/pdbsum/7taa PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 15:		Line 15:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=7taa ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 7taa" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 34:		Line 35:
	</StructureSection>		</StructureSection>
	[[Category: Alpha-amylase]]		[[Category: Alpha-amylase]]
-	[[Category: ~~Aspergillus oryzae~~]]	+	[[Category: Aspoz]]
	[[Category: Brzozowski, A M]]		[[Category: Brzozowski, A M]]
	[[Category: Davies, G J]]		[[Category: Davies, G J]]

OCA at 16:58, 10 December 2014

2014-12-10T16:58:24Z

←Older revision		Revision as of 16:58, 10 December 2014
Line 1:		Line 1:
-	[[~~Image~~:7taa.~~png\|left~~\|200px]]	+	==FAMILY 13 ALPHA AMYLASE IN COMPLEX WITH ACARBOSE==
		+	<StructureSection load='7taa' size='340' side='right' caption='[[7taa]], [[Resolution\|resolution]] 1.98Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[7taa]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aspergillus_oryzae Aspergillus oryzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7TAA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=7TAA FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ABC:MODIFIED+ACARBOSE+HEXASACCHARIDE'>ABC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alpha-amylase Alpha-amylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.1 3.2.1.1] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=7taa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7taa OCA], [http://www.rcsb.org/pdb/explore.do?structureId=7taa RCSB], [http://www.ebi.ac.uk/pdbsum/7taa PDBsum]</span></td></tr>
		+	</table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ta/7taa_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The three-dimensional structure of the Aspergillus oryzae alpha-amylase (TAKA-amylase), in complex with the inhibitor acarbose, has been determined by X-ray crystallography at a resolution of 1. 98 A. The tetrasaccharide inhibitor is present as a hexasaccharide presumably resulting from a transglycosylation event. The hexasaccharide occupies the -3 to +3 subsites of the enzyme, consistent with the known number of subsites determined by kinetic studies, with the acarbose unit itself in the -1 to +3 subsites of the enzyme. The transition state mimicking unsaturated pseudo-saccharide occupies the -1 subsite as expected and is present in a distorted 2H3 half-chair conformation. Careful refinement plus extremely well-resolved unbiased electron density suggest that the hexasaccharide represents a genuine transglycosylation product, but the possibility that this apparent species results from an overlapping network of tetrasaccharides is also discussed. Catalysis by alpha-amylase involves the hydrolysis of the alpha-1,4 linkages in amylose with a net retention of the anomeric configuration, via a double-displacement mechanism, as originally outlined by Koshland [Koshland, D. E. (1953) Biol. Rev. 28, 416-336]. The enzymatic acid/base and nucleophile, residues Glu230 and Asp206, respectively, are appropriately positioned for catalysis in this complex, and the hexasaccharide species allows mapping of all the noncovalent interactions between protein and ligand through the enzyme's six subsites.

-	~~{{STRUCTURE_7taa\| PDB=7taa \| SCENE= }}~~	+	Structure of the Aspergillus oryzae alpha-amylase complexed with the inhibitor acarbose at 2.0 A resolution.,Brzozowski AM, Davies GJ Biochemistry. 1997 Sep 9;36(36):10837-45. PMID:9283074<ref>PMID:9283074</ref>

-	~~===FAMILY 13 ALPHA AMYLASE IN COMPLEX WITH ACARBOSE===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_9283074}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[7taa]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Aspergillus_oryzae Aspergillus oryzae]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=7TAA OCA].~~	+

	==See Also==		==See Also==
-	*[[~~Alpha-~~Amylase\|~~Alpha-~~Amylase]]	+	*[[Amylase\|Amylase]]
-		+	*[[User:Gabriel Pons/Sandbox 2\|User:Gabriel Pons/Sandbox 2]]
-	==~~Reference~~==	+	== References ==
-	<~~ref group="xtra">PMID:009283074<~~/~~ref~~><~~references group="xtra"~~/>	+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Alpha-amylase]]		[[Category: Alpha-amylase]]
	[[Category: Aspergillus oryzae]]		[[Category: Aspergillus oryzae]]
-	[[Category: Brzozowski, A M.]]	+	[[Category: Brzozowski, A M]]
-	[[Category: Davies, G J.]]	+	[[Category: Davies, G J]]
	[[Category: Acarbose]]		[[Category: Acarbose]]
	[[Category: Amylase]]		[[Category: Amylase]]

OCA at 08:35, 20 October 2012

2012-10-20T08:35:26Z

←Older revision		Revision as of 08:35, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[7taa]] is a 1 chain structure ~~of [[Alpha-Amylase]]~~ with sequence from [http://en.wikipedia.org/wiki/Aspergillus_oryzae Aspergillus oryzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7TAA OCA].	+	[[7taa]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aspergillus_oryzae Aspergillus oryzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7TAA OCA].

	==See Also==		==See Also==

OCA: Protected "7taa" [edit=sysop:move=sysop]

2012-07-25T20:01:29Z

Protected "7taa" [edit=sysop:move=sysop]

←Older revision

Revision as of 20:01, 25 July 2012

OCA at 20:01, 25 July 2012

2012-07-25T20:01:25Z

←Older revision		Revision as of 20:01, 25 July 2012
Line 1:		Line 1:
-	{{Seed}}
	[[Image:7taa.png\|left\|200px]]		[[Image:7taa.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_7taa", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_7taa\| PDB=7taa \| SCENE= }}		{{STRUCTURE_7taa\| PDB=7taa \| SCENE= }}

	===FAMILY 13 ALPHA AMYLASE IN COMPLEX WITH ACARBOSE===		===FAMILY 13 ALPHA AMYLASE IN COMPLEX WITH ACARBOSE===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_9283074}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 9283074 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_9283074}}		{{ABSTRACT_PUBMED_9283074}}

	==About this Structure==		==About this Structure==
-	~~7TAA~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Aspergillus_oryzae Aspergillus oryzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7TAA OCA].	+	[[7taa]] is a 1 chain structure of [[Alpha-Amylase]] with sequence from [http://en.wikipedia.org/wiki/Aspergillus_oryzae Aspergillus oryzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7TAA OCA].
		+
		+	==See Also==
		+	*[[Alpha-Amylase\|Alpha-Amylase]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~9283074~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:009283074</ref><references group="xtra"/>
	[[Category: Alpha-amylase]]		[[Category: Alpha-amylase]]
	[[Category: Aspergillus oryzae]]		[[Category: Aspergillus oryzae]]
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	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Taka]]		[[Category: Taka]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 18:07:55 2009''

OCA at 16:07, 16 February 2009

2009-02-16T16:07:58Z

←Older revision		Revision as of 16:07, 16 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	7TAA is a ~~[[Single protein]]~~ structure of sequence from [http://en.wikipedia.org/wiki/Aspergillus_oryzae Aspergillus oryzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7TAA OCA].	+	7TAA is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Aspergillus_oryzae Aspergillus oryzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7TAA OCA].

	==Reference==		==Reference==
-	~~Structure of the Aspergillus oryzae alpha-amylase complexed with the inhibitor acarbose at 2.0 A resolution., Brzozowski AM, Davies GJ, Biochemistry. 1997 Sep 9;36(36):10837-45.~~ PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/9283074 9283074]~~	+	<ref group="xtra">PMID:9283074</ref><references group="xtra"/>
	[[Category: Alpha-amylase]]		[[Category: Alpha-amylase]]
	[[Category: Aspergillus oryzae]]		[[Category: Aspergillus oryzae]]
-	[[Category: Single protein]]
	[[Category: Brzozowski, A M.]]		[[Category: Brzozowski, A M.]]
	[[Category: Davies, G J.]]		[[Category: Davies, G J.]]
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	[[Category: Taka]]		[[Category: Taka]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Thu Jul 3 15~~:05:~~33 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 18:07:55 2009''

OCA at 12:05, 3 July 2008

2008-07-03T12:05:51Z

←Older revision		Revision as of 12:05, 3 July 2008
Line 1:		Line 1:
-	[[Image:7taa.~~gif~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:7taa.png\|left\|200px]]

	<!--		<!--
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	{{STRUCTURE_7taa\| PDB=7taa \| SCENE= }}		{{STRUCTURE_7taa\| PDB=7taa \| SCENE= }}

-	~~'''~~FAMILY 13 ALPHA AMYLASE IN COMPLEX WITH ACARBOSE~~'''~~	+	===FAMILY 13 ALPHA AMYLASE IN COMPLEX WITH ACARBOSE===


-	~~==Overview==~~	+	<!--
-	~~The three~~-~~dimensional structure of the Aspergillus oryzae alpha~~-~~amylase (TAKA-amylase), in complex with the inhibitor acarbose, has been determined by X-ray crystallography at a resolution of 1. 98 A.~~ The ~~tetrasaccharide inhibitor is present as a hexasaccharide presumably resulting from a transglycosylation event. The hexasaccharide occupies the -3 to +3 subsites of the enzyme~~, ~~consistent with the known number of subsites determined by kinetic studies~~, ~~with~~ the ~~acarbose unit itself in the -1~~ to ~~+3 subsites of~~ the ~~enzyme. The transition state mimicking unsaturated pseudo-saccharide occupies the -1 subsite~~ as ~~expected and is present in a distorted 2H3 half-chair conformation~~. ~~Careful refinement plus extremely well-resolved unbiased electron density suggest that the hexasaccharide represents a genuine transglycosylation product~~, ~~but~~ the ~~possibility that this apparent species results from an overlapping network of tetrasaccharides is also discussed~~. ~~Catalysis by alpha~~-~~amylase involves the hydrolysis of the alpha~~-1,4 linkages in amylose with a net retention of the anomeric configuration, via a double-displacement mechanism, as originally outlined by Koshland [Koshland, D. E. (1953) Biol. Rev. 28, 416-336]. The enzymatic acid/base and nucleophile, residues Glu230 and Asp206, respectively, are appropriately positioned for catalysis in this complex, and the hexasaccharide species allows mapping of all the noncovalent interactions between protein and ligand through the enzyme's six subsites.	+	The line below this paragraph, {{ABSTRACT_PUBMED_9283074}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 9283074 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_9283074}}

	==About this Structure==		==About this Structure==
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	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Taka]]		[[Category: Taka]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sun May~~ ~~4 22~~:47:46 2008''	+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jul 3 15:05:33 2008''

OCA at 19:47, 4 May 2008

2008-05-04T19:47:48Z

←Older revision		Revision as of 19:47, 4 May 2008
Line 1:		Line 1:
	[[Image:7taa.gif\|left\|200px]]		[[Image:7taa.gif\|left\|200px]]

-	~~{{Structure~~	+	<!--
-	~~\|PDB= 7taa \|SIZE=350\|CAPTION= <scene name='initialview01'>7taa</scene>~~, ~~resolution 1~~.~~98Å~~	+	The line below this paragraph, containing "STRUCTURE_7taa", creates the "Structure Box" on the page.
-	~~\|SITE=~~	+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	~~\|LIGAND= <~~scene ~~name='pdbligand=ABC:MODIFIED+ACARBOSE+HEXASACCHARIDE'>ABC</scene>~~, ~~<scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>~~	+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	~~\|ACTIVITY= <span class='plainlinks'>[http://en~~.~~wikipedia.org/wiki/Alpha~~-~~amylase Alpha~~-~~amylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.1 3.2.1.1] </span~~>	+	or leave the SCENE parameter empty for the default display.
-	\|~~GENE~~=	+	-->
-	\|~~DOMAIN~~=	+	{{STRUCTURE_7taa\| PDB=7taa \| SCENE= }}
-	~~\|RELATEDENTRY=~~	+
-	\|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=7taa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7taa OCA], [http://www.ebi.ac.uk/pdbsum/7taa PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=7taa RCSB]</span>	+
-	}}	+

	'''FAMILY 13 ALPHA AMYLASE IN COMPLEX WITH ACARBOSE'''		'''FAMILY 13 ALPHA AMYLASE IN COMPLEX WITH ACARBOSE'''
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	[[Category: Brzozowski, A M.]]		[[Category: Brzozowski, A M.]]
	[[Category: Davies, G J.]]		[[Category: Davies, G J.]]
-	[[Category: ~~acarbose~~]]	+	[[Category: Acarbose]]
-	[[Category: ~~amylase~~]]	+	[[Category: Amylase]]
-	[[Category: ~~glycosyl~~ hydrolase]]	+	[[Category: Glycosyl hydrolase]]
-	[[Category: ~~hydrolase~~]]	+	[[Category: Hydrolase]]
-	[[Category: ~~taka~~]]	+	[[Category: Taka]]
-		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 22:47:46 2008''
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Mon Mar 31 05~~:44:45 2008''	+

OCA at 02:44, 31 March 2008

2008-03-31T02:44:46Z

←Older revision		Revision as of 02:44, 31 March 2008
Line 4:		Line 4:
	\|PDB= 7taa \|SIZE=350\|CAPTION= <scene name='initialview01'>7taa</scene>, resolution 1.98Å		\|PDB= 7taa \|SIZE=350\|CAPTION= <scene name='initialview01'>7taa</scene>, resolution 1.98Å
	\|SITE=		\|SITE=
-	\|LIGAND= <scene name='pdbligand=ABC:MODIFIED+ACARBOSE+HEXASACCHARIDE'>ABC</scene> ~~and~~ <scene name='pdbligand=CA:CALCIUM ION'>CA</scene>	+	\|LIGAND= <scene name='pdbligand=ABC:MODIFIED+ACARBOSE+HEXASACCHARIDE'>ABC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>
-	\|ACTIVITY= [http://en.wikipedia.org/wiki/Alpha-amylase Alpha-amylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.1 3.2.1.1]	+	\|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Alpha-amylase Alpha-amylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.1 3.2.1.1] </span>
	\|GENE=		\|GENE=
		+	\|DOMAIN=
		+	\|RELATEDENTRY=
		+	\|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=7taa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7taa OCA], [http://www.ebi.ac.uk/pdbsum/7taa PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=7taa RCSB]</span>
	}}		}}

Line 25:		Line 28:
	[[Category: Brzozowski, A M.]]		[[Category: Brzozowski, A M.]]
	[[Category: Davies, G J.]]		[[Category: Davies, G J.]]
-	[[Category: ABC]]
-	[[Category: CA]]
	[[Category: acarbose]]		[[Category: acarbose]]
	[[Category: amylase]]		[[Category: amylase]]
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	[[Category: taka]]		[[Category: taka]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Thu~~ Mar ~~20 19~~:15:06 2008''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:44:45 2008''

OCA at 17:15, 20 March 2008

2008-03-20T17:15:07Z

←Older revision		Revision as of 17:15, 20 March 2008
Line 1:		Line 1:
-	[[Image:7taa.gif\|left\|200px]]~~<br /><applet load~~="7taa~~" size~~="350~~" color~~=~~"white" frame="true" align~~=~~"right" spinBox="true"~~	+	[[Image:7taa.gif\|left\|200px]]
-	~~caption="~~7taa, resolution 1.98Å" />	+
-	'''FAMILY 13 ALPHA AMYLASE IN COMPLEX WITH ACARBOSE'''~~<br />~~	+	{{Structure
		+	\|PDB= 7taa \|SIZE=350\|CAPTION= <scene name='initialview01'>7taa</scene>, resolution 1.98Å
		+	\|SITE=
		+	\|LIGAND= <scene name='pdbligand=ABC:MODIFIED+ACARBOSE+HEXASACCHARIDE'>ABC</scene> and <scene name='pdbligand=CA:CALCIUM ION'>CA</scene>
		+	\|ACTIVITY= [http://en.wikipedia.org/wiki/Alpha-amylase Alpha-amylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.1 3.2.1.1]
		+	\|GENE=
		+	}}
		+
		+	'''FAMILY 13 ALPHA AMYLASE IN COMPLEX WITH ACARBOSE'''
		+

	==Overview==		==Overview==
Line 7:		Line 16:

	==About this Structure==		==About this Structure==
-	7TAA is a [~~http://en.wikipedia.org/wiki/Single_protein~~ Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aspergillus_oryzae Aspergillus oryzae] ~~with <scene name='pdbligand=ABC:'>ABC</scene> and <scene name='pdbligand=CA:'>CA</scene> as [http://en~~.~~wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Alpha-amylase Alpha-amylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.1 3.2.1.1]~~ Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7TAA OCA].	+	7TAA is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Aspergillus_oryzae Aspergillus oryzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7TAA OCA].

	==Reference==		==Reference==
-	Structure of the Aspergillus oryzae alpha-amylase complexed with the inhibitor acarbose at 2.0 A resolution., Brzozowski AM, Davies GJ, Biochemistry. 1997 Sep 9;36(36):10837-45. PMID:[http://~~ispc~~.~~weizmann~~.ac.~~il/~~/~~pmbin~~/~~getpm?pmid=~~9283074 9283074]	+	Structure of the Aspergillus oryzae alpha-amylase complexed with the inhibitor acarbose at 2.0 A resolution., Brzozowski AM, Davies GJ, Biochemistry. 1997 Sep 9;36(36):10837-45. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9283074 9283074]
	[[Category: Alpha-amylase]]		[[Category: Alpha-amylase]]
	[[Category: Aspergillus oryzae]]		[[Category: Aspergillus oryzae]]
Line 24:		Line 33:
	[[Category: taka]]		[[Category: taka]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu ~~Feb 21~~ 19:17:34 2008''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 19:15:06 2008''