7th5 - Revision history

OCA at 09:45, 25 October 2023

2023-10-25T09:45:20Z

←Older revision		Revision as of 09:45, 25 October 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7th5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7TH5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7TH5 FirstGlance]. <br>		<table><tr><td colspan='2'>[[7th5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7TH5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7TH5 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.09Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7th5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7th5 OCA], [https://pdbe.org/7th5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7th5 RCSB], [https://www.ebi.ac.uk/pdbsum/7th5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7th5 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7th5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7th5 OCA], [https://pdbe.org/7th5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7th5 RCSB], [https://www.ebi.ac.uk/pdbsum/7th5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7th5 ProSAT]</span></td></tr>
	</table>		</table>
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	</div>		</div>
	<div class="pdbe-citations 7th5" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 7th5" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Methylenetetrahydrofolate reductase 3D structures\|Methylenetetrahydrofolate reductase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 07:15, 18 January 2023

2023-01-18T07:15:40Z

←Older revision		Revision as of 07:15, 18 January 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 7th5 is ~~ON HOLD until Paper~~ Publication	+	==Thermus thermophilus methylenetetrahydrofolate reductase==
		+	<StructureSection load='7th5' size='340' side='right'caption='[[7th5]], [[Resolution\|resolution]] 2.09Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[7th5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7TH5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7TH5 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7th5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7th5 OCA], [https://pdbe.org/7th5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7th5 RCSB], [https://www.ebi.ac.uk/pdbsum/7th5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7th5 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q5SLG6_THET8 Q5SLG6_THET8]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The flavoprotein methylenetetrahydrofolate reductase (MTHFR) catalyzes the reduction of N5, N10-methylenetetrahydrofolate (CH(2)-H(4)folate) to N5-methyltetrahydrofolate (CH(3)-H(4)folate), committing a methyl group from the folate cycle to the methionine one. This committed step is the sum of multiple ping-pong electron transfers involving multiple substrates, intermediates, and products all sharing the same active site. Insight into folate substrate binding is needed to better understand this multifunctional active site. Here, we performed activity assays with Thermus thermophilus MTHFR (tMTHFR), which showed pH-dependent inhibition by the substrate analog, N5-formyltetrahydrofolate (CHO-H(4)folate). Our crystal structure of a tMTHFR*CHO-H(4)folate complex revealed a unique folate-binding mode; tMTHFR subtly rearranges its active site to form a distinct folate binding environment. Formation of a novel binding pocket for the CHO-H(4)folate p-aminobenzoic acid (PABA) moiety directly affects how bent the folate ligand is and its accommodation in the active site. Comparative analysis of the available active (FAD- and folate-bound) MTHFR complex structures reveals that CHO-H(4)folate is accommodated in the active site in a conformation that would not support hydride transfer, but rather in a conformation that potentially reports on a different step in the reaction mechanism after this committed step, such as CH(2)-H(4)folate ring-opening. This active site remodeling provides insights into the functional relevance of the differential folate binding modes and their potential roles in the catalytic cycle. The conformational flexibility displayed by tMTHFR demonstrates how a shared active site can use a few amino acid residues in lieu of extra domains to accommodate chemically-distinct moieties and functionalities.

-	~~Authors: Yamada~~, K., ~~Koutmos~~, M.	+	5-Formyltetrahydrofolate Promotes Conformational Remodeling in a Methylenetetrahydrofolate Reductase Active Site and Inhibits Its Activity.,Yamada K, Mendoza J, Koutmos M J Biol Chem. 2022 Dec 30:102855. doi: 10.1016/j.jbc.2022.102855. PMID:36592927<ref>PMID:36592927</ref>

-	~~Description~~: ~~Thermus thermophilus methylenetetrahydrofolate reductase~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: ~~Yamada, K~~]]	+	<div class="pdbe-citations 7th5" style="background-color:#fffaf0;"></div>
-	[[Category: Koutmos, M]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Thermus thermophilus HB8]]
		+	[[Category: Koutmos M]]
		+	[[Category: Yamada K]]

OCA at 09:06, 16 February 2022

2022-02-16T09:06:56Z

←Older revision		Revision as of 09:06, 16 February 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7th5 is ON HOLD	+	The entry 7th5 is ON HOLD until Paper Publication

	Authors: Yamada, K., Koutmos, M.		Authors: Yamada, K., Koutmos, M.

OCA: Protected "7th5" [edit=sysop:move=sysop]

2022-01-19T06:02:07Z

Protected "7th5" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:02, 19 January 2022

OCA: New page: '''Unreleased structure''' The entry 7th5 is ON HOLD Authors: Yamada, K., Koutmos, M. Description: Thermus thermophilus methylenetetrahydrofolate reductase [[Category: Unreleased Struc...

2022-01-19T06:02:06Z

New page: '''Unreleased structure''' The entry 7th5 is ON HOLD Authors: Yamada, K., Koutmos, M. Description: Thermus thermophilus methylenetetrahydrofolate reductase [[Category: Unreleased Struc...

New page

'''Unreleased structure'''

The entry 7th5 is ON HOLD

Authors: Yamada, K., Koutmos, M.

Description: Thermus thermophilus methylenetetrahydrofolate reductase
[[Category: Unreleased Structures]]
[[Category: Yamada, K]]
[[Category: Koutmos, M]]