7tlo - Revision history

OCA at 17:05, 18 October 2023

2023-10-18T17:05:24Z

←Older revision		Revision as of 17:05, 18 October 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7tlo]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_marinum_ATCC_BAA-535 Mycobacterium marinum ATCC BAA-535]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7TLO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7TLO FirstGlance]. <br>		<table><tr><td colspan='2'>[[7tlo]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_marinum_ATCC_BAA-535 Mycobacterium marinum ATCC BAA-535]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7TLO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7TLO FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.55Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7tlo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7tlo OCA], [https://pdbe.org/7tlo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7tlo RCSB], [https://www.ebi.ac.uk/pdbsum/7tlo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7tlo ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7tlo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7tlo OCA], [https://pdbe.org/7tlo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7tlo RCSB], [https://www.ebi.ac.uk/pdbsum/7tlo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7tlo ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/A0A2Z5YMP0_MYCMR A0A2Z5YMP0_MYCMR]]	+	[https://www.uniprot.org/uniprot/A0A2Z5YMP0_MYCMR A0A2Z5YMP0_MYCMR]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 7tlo" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 7tlo" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Cytochrome P450 3D structures\|Cytochrome P450 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 06:55, 14 September 2022

2022-09-14T06:55:21Z

←Older revision		Revision as of 06:55, 14 September 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7tlo is ~~ON HOLD until Paper~~ Publication	+	==X-ray crystal structure of substrate free cytochrome P450 CYP142A3 from Mycobacterium Marinum==
		+	<StructureSection load='7tlo' size='340' side='right'caption='[[7tlo]], [[Resolution\|resolution]] 1.55Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[7tlo]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_marinum_ATCC_BAA-535 Mycobacterium marinum ATCC BAA-535]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7TLO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7TLO FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7tlo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7tlo OCA], [https://pdbe.org/7tlo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7tlo RCSB], [https://www.ebi.ac.uk/pdbsum/7tlo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7tlo ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/A0A2Z5YMP0_MYCMR A0A2Z5YMP0_MYCMR]]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The steroid binding CYP142 cytochrome P450 enzymes of Mycobacterium species are involved in the metabolism of cholesterol and its derivatives. The equivalent enzyme from Mycobacterium ulcerans was studied to compare the degree of functional conservation between members of this CYP family. We compared substrate binding of the CYP142A3 enzymes of M. ulcerans and M. marinum and CYP142A1 from M. tuberculosis using UV-vis spectroscopy. The catalytic oxidation of cholesterol derivatives by all three enzymes was undertaken. Both CYP142A3 enzymes were structurally characterized by X-ray crystallography. The amino acid sequences of the CYP142A3 enzymes are more similar to CYP142A1 from M. tuberculosis than CYP142A2 from Mycolicibacterium smegmatis. Both CYP142A3 enzymes have substrate binding properties, which are more resemblant to CYP142A1 than CYP142A2. The cholest-4-en-3-one-bound X-ray crystal structure of both CYP142A3 enzymes were determined at a resolution of <1.8 A, revealing the substrate binding mode at a high level of detail. The structures of the cholest-4-en-3-one binding CYP142 enzymes from M. ulcerans and M. marinum demonstrate how the steroid binds in the active site of these enzymes. They provide an explanation for the high selectivity of the enzyme for terminal methyl C-H bond oxidation to form 26-hydroxy derivatives. These enzymes in pathogenic Mycobacterium species are candidates for inhibition. The work here demonstrates that similar drug molecules could target these CYP142 enzymes from different species in order to combat Buruli ulcer or tuberculosis.

-	~~Authors: Ghith~~, A., Bruning, ~~J.B.~~, ~~Bell~~, S.G.	+	The Structures of the Steroid Binding CYP142 Cytochrome P450 Enzymes from Mycobacterium ulcerans and Mycobacterium marinum.,Ghith A, Doherty DZ, Bruning JB, Russell RA, De Voss JJ, Bell SG ACS Infect Dis. 2022 Aug 12;8(8):1606-1617. doi: 10.1021/acsinfecdis.2c00215., Epub 2022 Jul 26. PMID:35881654<ref>PMID:35881654</ref>

-	~~Description: X-ray crystal structure~~ of ~~substrate free cytochrome P450 CYP142A3 from Mycobacterium Marinum~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: ~~Bruning, J.B~~]]	+	<div class="pdbe-citations 7tlo" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Ghith, A~~]]	+	== References ==
-	[[Category: ~~Bell, S.G~~]]	+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Mycobacterium marinum ATCC BAA-535]]
		+	[[Category: Bell SG]]
		+	[[Category: Bruning JB]]
		+	[[Category: Ghith A]]

OCA at 06:30, 23 February 2022

2022-02-23T06:30:29Z

←Older revision		Revision as of 06:30, 23 February 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7tlo is ON HOLD	+	The entry 7tlo is ON HOLD until Paper Publication

	Authors: Ghith, A., Bruning, J.B., Bell, S.G.		Authors: Ghith, A., Bruning, J.B., Bell, S.G.

OCA: Protected "7tlo" [edit=sysop:move=sysop]

2022-01-27T05:35:08Z

Protected "7tlo" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:35, 27 January 2022

OCA: New page: '''Unreleased structure''' The entry 7tlo is ON HOLD Authors: Ghith, A., Bruning, J.B., Bell, S.G. Description: X-ray crystal structure of substrate free cytochrome P450 CYP142A3 from ...

2022-01-27T05:35:07Z

New page: '''Unreleased structure''' The entry 7tlo is ON HOLD Authors: Ghith, A., Bruning, J.B., Bell, S.G. Description: X-ray crystal structure of substrate free cytochrome P450 CYP142A3 from ...

New page

'''Unreleased structure'''

The entry 7tlo is ON HOLD

Authors: Ghith, A., Bruning, J.B., Bell, S.G.

Description: X-ray crystal structure of substrate free cytochrome P450 CYP142A3 from Mycobacterium Marinum
[[Category: Unreleased Structures]]
[[Category: Bruning, J.B]]
[[Category: Ghith, A]]
[[Category: Bell, S.G]]