OCA at 21:13, 28 June 2023

2023-06-28T21:13:22Z

←Older revision		Revision as of 21:13, 28 June 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 7u0i is ~~ON HOLD until Paper~~ Publication	+	==Structure of LIN28b nucleosome bound 2 OCT4==
		+	<StructureSection load='7u0i' size='340' side='right'caption='[[7u0i]], [[Resolution\|resolution]] 2.60Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[7u0i]] is a 14 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12], [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7U0I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7U0I FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 2.6Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7u0i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7u0i OCA], [https://pdbe.org/7u0i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7u0i RCSB], [https://www.ebi.ac.uk/pdbsum/7u0i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7u0i ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/H2A2C_HUMAN H2A2C_HUMAN] Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Pioneer transcription factors are essential for cell fate changes by targeting closed chromatin. OCT4 is a crucial pioneer factor that can induce cell reprogramming. However, the structural basis of how pioneer factors recognize the in vivo nucleosomal DNA targets is unknown. Here, we determine the high-resolution structures of the nucleosome containing human LIN28B DNA and its complexes with the OCT4 DNA binding region. Three OCT4s bind the pre-positioned nucleosome by recognizing non-canonical DNA sequences. Two use their POUS domains while the other uses the POUS-loop-POUHD region; POUHD serves as a wedge to unwrap approximately 25 base pair DNA. Our analysis of previous genomic data and determination of the ESRRB-nucleosome-OCT4 structure confirmed the generality of these structural features. Moreover, biochemical studies suggest that multiple OCT4s cooperatively open the H1-condensed nucleosome array containing the LIN28B nucleosome. Thus, our study suggests a mechanism of how OCT4 can target the nucleosome and open closed chromatin.

-	~~Authors~~:	+	Structural mechanism of LIN28B nucleosome targeting by OCT4.,Guan R, Lian T, Zhou BR, Wheeler D, Bai Y Mol Cell. 2023 Jun 15;83(12):1970-1982.e6. doi: 10.1016/j.molcel.2023.05.030. PMID:37327775<ref>PMID:37327775</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 7u0i" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Escherichia coli K-12]]
		+	[[Category: Homo sapiens]]
		+	[[Category: Large Structures]]
		+	[[Category: Mus musculus]]
		+	[[Category: Ruifang G]]
		+	[[Category: Tengfei L]]
		+	[[Category: Yawen B]]

OCA at 09:29, 30 March 2022

2022-03-30T09:29:36Z

←Older revision		Revision as of 09:29, 30 March 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7u0i is ON HOLD	+	The entry 7u0i is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7u0i" [edit=sysop:move=sysop]

2022-03-02T05:06:13Z

Protected "7u0i" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:06, 2 March 2022

OCA: New page: '''Unreleased structure''' The entry 7u0i is ON HOLD Authors: Description: Category: Unreleased Structures

2022-03-02T05:06:12Z

New page: '''Unreleased structure''' The entry 7u0i is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7u0i is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7u0i - Revision history

OCA at 21:13, 28 June 2023

OCA at 09:29, 30 March 2022

OCA: Protected "7u0i" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7u0i is ON HOLD Authors: Description: Category: Unreleased Structures