7ucl - Revision history

OCA at 17:14, 18 October 2023

2023-10-18T17:14:00Z

←Older revision		Revision as of 17:14, 18 October 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7ucl]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Cylindrospermopsis_raciborskii Cylindrospermopsis raciborskii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7UCL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7UCL FirstGlance]. <br>		<table><tr><td colspan='2'>[[7ucl]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Cylindrospermopsis_raciborskii Cylindrospermopsis raciborskii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7UCL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7UCL FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.09Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ucl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ucl OCA], [https://pdbe.org/7ucl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ucl RCSB], [https://www.ebi.ac.uk/pdbsum/7ucl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ucl ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ucl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ucl OCA], [https://pdbe.org/7ucl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ucl RCSB], [https://www.ebi.ac.uk/pdbsum/7ucl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ucl ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/B3EYF9_9CYAN B3EYF9_9CYAN]]	+	[https://www.uniprot.org/uniprot/B3EYF9_9CYAN B3EYF9_9CYAN]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 19:50, 7 September 2022

2022-09-07T19:50:07Z

←Older revision		Revision as of 19:50, 7 September 2022
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	==SxtA Methyltransferase variant F458H in complex with Mn2+ and malonate==		==SxtA Methyltransferase variant F458H in complex with Mn2+ and malonate==
-	<StructureSection load='7ucl' size='340' side='right'caption='[[7ucl]]' scene=''>	+	<StructureSection load='7ucl' size='340' side='right'caption='[[7ucl]], [[Resolution\|resolution]] 2.09Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7UCL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7UCL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7ucl]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Cylindrospermopsis_raciborskii Cylindrospermopsis raciborskii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7UCL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7UCL FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ucl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ucl OCA], [https://pdbe.org/7ucl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ucl RCSB], [https://www.ebi.ac.uk/pdbsum/7ucl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ucl ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ucl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ucl OCA], [https://pdbe.org/7ucl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ucl RCSB], [https://www.ebi.ac.uk/pdbsum/7ucl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ucl ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/B3EYF9_9CYAN B3EYF9_9CYAN]]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Installation of methyl groups can significantly improve the binding of small-molecule drugs to protein targets; however, site-selective methylation often presents a significant synthetic challenge. Metal- and S-adenosyl-methionine (SAM)-dependent methyltransferases (MTs) in natural-product biosynthetic pathways are powerful enzymatic tools for selective or chemically challenging C-methylation reactions. Each of these MTs selectively catalyzes one or two methyl transfer reactions. Crystal structures and biochemical assays of the Mn(2+)-dependent monomethyltransferase from the saxitoxin biosynthetic pathway (SxtA MT) revealed the structural basis for control of methylation extent. The SxtA monomethyltransferase was converted to a dimethyltransferase by modification of the metal binding site, addition of an active site base, and an amino acid substitution to provide space in the substrate pocket for two methyl substituents. A reciprocal change converted a related dimethyltransferase into a monomethyltransferase, supporting our hypothesis that steric hindrance can prevent a second methylation event. A novel understanding of MTs will accelerate the development of MT-based catalysts and MT engineering for use in small-molecule synthesis.
		+
		+	Structural Basis for Control of Methylation Extent in Polyketide Synthase Metal-Dependent C-Methyltransferases.,Lao Y, Skiba MA, Chun SW, Narayan ARH, Smith JL ACS Chem Biol. 2022 Aug 19;17(8):2088-2098. doi: 10.1021/acschembio.2c00085. Epub, 2022 May 20. PMID:35594521<ref>PMID:35594521</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7ucl" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Cylindrospermopsis raciborskii]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Lao Y]]		[[Category: Lao Y]]
	[[Category: Smith JL]]		[[Category: Smith JL]]

OCA at 06:44, 31 August 2022

2022-08-31T06:44:33Z

←Older revision		Revision as of 06:44, 31 August 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7ucl ~~is ON HOLD until Paper Publication~~	+	==SxtA Methyltransferase variant F458H in complex with Mn2+ and malonate==
-		+	<StructureSection load='7ucl' size='340' side='right'caption='[[7ucl]]' scene=''>
-	~~Authors~~: ~~Lao~~, Y., ~~Smith~~, J.L.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7UCL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7UCL FirstGlance]. <br>
-	~~Description: SxtA Methyltransferase variant F458H in complex with Mn2+ and malonate~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ucl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ucl OCA], [https://pdbe.org/7ucl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ucl RCSB], [https://www.ebi.ac.uk/pdbsum/7ucl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ucl ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: Lao, Y]]	+	__TOC__
-	[[Category: Smith~~, J.L~~]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Lao Y]]
		+	[[Category: Smith JL]]

OCA at 04:43, 12 May 2022

2022-05-12T04:43:43Z

←Older revision		Revision as of 04:43, 12 May 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7ucl is ON HOLD	+	The entry 7ucl is ON HOLD until Paper Publication

	Authors: Lao, Y., Smith, J.L.		Authors: Lao, Y., Smith, J.L.

OCA: Protected "7ucl" [edit=sysop:move=sysop]

2022-03-23T09:54:10Z

Protected "7ucl" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:54, 23 March 2022

OCA: New page: '''Unreleased structure''' The entry 7ucl is ON HOLD Authors: Lao, Y., Smith, J.L. Description: SxtA Methyltransferase variant F458H in complex with Mn2+ and malonate [[Category: Unrel...

2022-03-23T09:54:08Z

New page: '''Unreleased structure''' The entry 7ucl is ON HOLD Authors: Lao, Y., Smith, J.L. Description: SxtA Methyltransferase variant F458H in complex with Mn2+ and malonate [[Category: Unrel...

New page

'''Unreleased structure'''

The entry 7ucl is ON HOLD

Authors: Lao, Y., Smith, J.L.

Description: SxtA Methyltransferase variant F458H in complex with Mn2+ and malonate
[[Category: Unreleased Structures]]
[[Category: Lao, Y]]
[[Category: Smith, J.L]]