OCA at 06:41, 8 February 2023

2023-02-08T06:41:19Z

←Older revision		Revision as of 06:41, 8 February 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 7uhz is ~~ON HOLD until Paper~~ Publication	+	==Post-fusion ectodomain of HSV-1 gB in complex with BMPC-23 Fab==
		+	<StructureSection load='7uhz' size='340' side='right'caption='[[7uhz]], [[Resolution\|resolution]] 3.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[7uhz]] is a 9 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_alphaherpesvirus_1_strain_KOS Human alphaherpesvirus 1 strain KOS] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7UHZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7UHZ FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7uhz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7uhz OCA], [https://pdbe.org/7uhz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7uhz RCSB], [https://www.ebi.ac.uk/pdbsum/7uhz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7uhz ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/GB_HHV1K GB_HHV1K] Envelope glycoprotein that forms spikes at the surface of virion envelope. Essential for the initial attachment to heparan sulfate moities of the host cell surface proteoglycans. Involved in fusion of viral and cellular membranes leading to virus entry into the host cell. Following initial binding of gD to one of its receptors, membrane fusion is mediated by the fusion machinery composed at least of gB and the heterodimer gH/gL. May also be involved in the fusion between the virion envelope and the outer nuclear membrane during virion egress. Also plays a role, together with gK, in virus-induced cell-to-cell fusion (syncytia formation).<ref>PMID:17299053</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	There is an unmet need for monoclonal antibodies (mAbs) for prevention or as adjunctive treatment of herpes simplex virus (HSV) disease. Most vaccine and mAb efforts focus on neutralizing antibodies, but for HSV this strategy has proven ineffective. Preclinical studies with a candidate HSV vaccine strain, DeltagD-2, demonstrated that non-neutralizing antibodies that activate Fcgamma receptors (FcgammaRs) to mediate antibody-dependent cellular cytotoxicity (ADCC) provide active and passive protection against HSV-1 and HSV-2. We hypothesized that this vaccine provides a tool to identify and characterize protective mAbs. We isolated HSV-specific mAbs from germinal center and memory B cells and bone marrow plasmacytes of DeltagD-2-vaccinated mice and evaluated these mAbs for binding, neutralizing, and FcgammaR-activating activity and for protective efficacy in mice. The most potent protective mAb, BMPC-23, was not neutralizing but activated murine FcgammaRIV, a biomarker of ADCC. The cryo-electron microscopic structure of the Fab-glycoprotein B (gB) assembly identified domain IV of gB as the epitope. A single dose of BMPC-23 administered 24 hours before or after viral challenge provided significant protection when configured as mouse IgG2c and protected mice expressing human FcgammaRIII when engineered as a human IgG1. These results highlight the importance of FcR-activating antibodies in protecting against HSV.

-	~~Authors~~:	+	A non-neutralizing glycoprotein B monoclonal antibody protects against herpes simplex virus disease in mice.,Kuraoka M, Aschner CB, Windsor IW, Mahant AM, Garforth SJ, Kong SL, Achkar JM, Almo SC, Kelsoe G, Herold BC J Clin Invest. 2023 Feb 1;133(3):e161968. doi: 10.1172/JCI161968. PMID:36454639<ref>PMID:36454639</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 7uhz" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Human alphaherpesvirus 1 strain KOS]]
		+	[[Category: Large Structures]]
		+	[[Category: Mus musculus]]
		+	[[Category: Almo SC]]
		+	[[Category: Garforth SJ]]
		+	[[Category: Harrison SC]]
		+	[[Category: Kong SL]]
		+	[[Category: Windsor IW]]

OCA at 11:29, 4 May 2022

2022-05-04T11:29:24Z

←Older revision		Revision as of 11:29, 4 May 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7uhz is ON HOLD	+	The entry 7uhz is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7uhz" [edit=sysop:move=sysop]

2022-04-06T09:04:59Z

Protected "7uhz" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:04, 6 April 2022

OCA: New page: '''Unreleased structure''' The entry 7uhz is ON HOLD Authors: Description: Category: Unreleased Structures

2022-04-06T09:04:58Z

New page: '''Unreleased structure''' The entry 7uhz is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7uhz is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7uhz - Revision history

OCA at 06:41, 8 February 2023

OCA at 11:29, 4 May 2022

OCA: Protected "7uhz" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7uhz is ON HOLD Authors: Description: Category: Unreleased Structures