OCA at 17:19, 18 October 2023

2023-10-18T17:19:18Z

←Older revision		Revision as of 17:19, 18 October 2023
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	==Pseudobacteroides cellulosolvens pseudo-CphB==		==Pseudobacteroides cellulosolvens pseudo-CphB==
-	<StructureSection load='7uqv' size='340' side='right'caption='[[7uqv]]' scene=''>	+	<StructureSection load='7uqv' size='340' side='right'caption='[[7uqv]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7UQV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7UQV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7uqv]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudobacteroides_cellulosolvens_ATCC_35603_=_DSM_2933 Pseudobacteroides cellulosolvens ATCC 35603 = DSM 2933]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7UQV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7UQV FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7uqv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7uqv OCA], [https://pdbe.org/7uqv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7uqv RCSB], [https://www.ebi.ac.uk/pdbsum/7uqv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7uqv ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.4Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7uqv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7uqv OCA], [https://pdbe.org/7uqv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7uqv RCSB], [https://www.ebi.ac.uk/pdbsum/7uqv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7uqv ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A0L6JSP1_9FIRM A0A0L6JSP1_9FIRM] Exopeptidase that catalyzes the hydrolytic cleavage of multi-L-arginyl-poly-L-aspartic acid (cyanophycin; a water-insoluble reserve polymer) into aspartate-arginine dipeptides.[ARBA:ARBA00002039]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	BACKGROUND: Cyanophycinases are serine protease family enzymes which are required for the metabolism of cyanophycin, the natural polymer multi-L-arginyl-poly(L-aspartic acid). Cyanophycinases degrade cyanophycin to beta-Asp-Arg dipeptides, which enables use of this important store of fixed nitrogen. METHODS: We used genetic code expansion to incorporate diaminopropionic acid into cyanophycinase in place of the active site serine, and determined a high-resolution structure of the covalent acyl-enzyme intermediate resulting from attack of cyanophycinase on a short cyanophycin segment. RESULTS: The structure indicates that cyanophycin dipeptide residues P1 and P1' bind shallow pockets adjacent to the catalytic residues. We observe many cyanophycinase - P1 dipeptide interactions in the co-complex structure. Calorimetry measurements show that at least two cyanophycin dipeptides are needed for high affinity binding to cyanophycinase. We also characterized a putative cyanophycinase which we found to be structurally very similar but that shows no activity and could not be activated by mutation of its active site. GENERAL SIGNIFICANCE: Despite its peptidic structure, cyanophycin is resistant to degradation by peptidases and other proteases. Our results help show how cyanophycinase can specifically bind and degrade this important polymer.
		+
		+	The structure of cyanophycinase in complex with a cyanophycin degradation intermediate.,Sharon I, Grogg M, Hilvert D, Schmeing TM Biochim Biophys Acta Gen Subj. 2022 Jul 26;1866(11):130217. doi:, 10.1016/j.bbagen.2022.130217. PMID:35905922<ref>PMID:35905922</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7uqv" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Pseudobacteroides cellulosolvens ATCC 35603 = DSM 2933]]
	[[Category: Schmeing TM]]		[[Category: Schmeing TM]]
	[[Category: Sharon I]]		[[Category: Sharon I]]

OCA at 05:00, 10 August 2022

2022-08-10T05:00:47Z

←Older revision		Revision as of 05:00, 10 August 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7uqv ~~is ON HOLD until Paper Publication~~	+	==Pseudobacteroides cellulosolvens pseudo-CphB==
-		+	<StructureSection load='7uqv' size='340' side='right'caption='[[7uqv]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7UQV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7UQV FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7uqv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7uqv OCA], [https://pdbe.org/7uqv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7uqv RCSB], [https://www.ebi.ac.uk/pdbsum/7uqv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7uqv ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Schmeing TM]]
		+	[[Category: Sharon I]]

OCA at 03:50, 8 June 2022

2022-06-08T03:50:49Z

←Older revision		Revision as of 03:50, 8 June 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7uqv is ON HOLD	+	The entry 7uqv is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7uqv" [edit=sysop:move=sysop]

2022-04-27T10:27:45Z

Protected "7uqv" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:27, 27 April 2022

OCA: New page: '''Unreleased structure''' The entry 7uqv is ON HOLD Authors: Description: Category: Unreleased Structures

2022-04-27T10:27:44Z

New page: '''Unreleased structure''' The entry 7uqv is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7uqv is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7uqv - Revision history

OCA at 17:19, 18 October 2023

OCA at 05:00, 10 August 2022

OCA at 03:50, 8 June 2022

OCA: Protected "7uqv" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7uqv is ON HOLD Authors: Description: Category: Unreleased Structures