7urh - Revision history

OCA at 09:56, 25 October 2023

2023-10-25T09:56:15Z

←Older revision		Revision as of 09:56, 25 October 2023
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[7urh]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7URH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7URH FirstGlance]. <br>		<table><tr><td colspan='2'>[[7urh]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7URH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7URH FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.468Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7urh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7urh OCA], [https://pdbe.org/7urh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7urh RCSB], [https://www.ebi.ac.uk/pdbsum/7urh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7urh ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7urh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7urh OCA], [https://pdbe.org/7urh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7urh RCSB], [https://www.ebi.ac.uk/pdbsum/7urh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7urh ProSAT]</span></td></tr>
	</table>		</table>

OCA at 16:56, 26 April 2023

2023-04-26T16:56:50Z

←Older revision		Revision as of 16:56, 26 April 2023
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-	'''Unreleased structure'''

-	~~The entry~~ 7urh is ~~ON HOLD until Paper~~ Publication	+	==Crystal structure of Ferritin 2 from Caenorhabditis elegans, FTN-2==
		+	<StructureSection load='7urh' size='340' side='right'caption='[[7urh]], [[Resolution\|resolution]] 1.47Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[7urh]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7URH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7URH FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7urh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7urh OCA], [https://pdbe.org/7urh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7urh RCSB], [https://www.ebi.ac.uk/pdbsum/7urh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7urh ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q9TYS3_CAEEL Q9TYS3_CAEEL] Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation.[RuleBase:RU361145]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The nematode Caenorhabditis elegans contains genes for two types of ferritin (ftn-1 and ftn-2) that express FTN-1 and FTN-2. We have expressed and purified both proteins and characterized them by X-ray crystallography, cryo-electron microscopy, transmission electron microscopy, dynamic light scattering, and kinetically by oxygen electrode and UV-vis spectroscopy. Both show ferroxidase activity, but although they have identical ferroxidase active sites, FTN-2 is shown to react approximately 10 times faster than FTN-1, with L-type ferritin character over longer time periods. We hypothesize that the large variation in rate may be due to differences in the three- and four-fold channels into the interior of the protein 24-mer. FTN-2 is shown to have a wider entrance into the three-fold channel than FTN-1. Additionally, the charge gradient through the channel of FTN-2 is more pronounced, with Asn and Gln residues in FTN-1 replaced by Asp and Glu residues in FTN-2. Both FTN-1 and FTN-2 have an Asn residue near the ferroxidase active site that is a Val in most other species, including human H ferritin. This Asn residue has been observed before in ferritin from the marine pennate diatom Pseudo-mitzchia multiseries. By replacing this Asn residue with a Val in FTN-2, we show that the reactivity decreases over long time scales. We therefore propose that Asn106 is involved in iron transport from the ferroxidase active site to the central cavity of the protein.

-	~~Authors~~:	+	Biochemical Characterization of Caenorhabditis elegans Ferritins.,Mubarak SSM, Malcolm TR, Brown HG, Hanssen E, Maher MJ, McColl G, Jameson GNL Biochemistry. 2023 Apr 4. doi: 10.1021/acs.biochem.3c00005. PMID:37014173<ref>PMID:37014173</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 7urh" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Caenorhabditis elegans]]
		+	[[Category: Large Structures]]
		+	[[Category: Maher MJ]]
		+	[[Category: Malcolm TR]]
		+	[[Category: Mubarak SSM]]

OCA at 04:46, 12 May 2022

2022-05-12T04:46:26Z

←Older revision		Revision as of 04:46, 12 May 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7urh is ON HOLD	+	The entry 7urh is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7urh" [edit=sysop:move=sysop]

2022-04-27T10:28:46Z

Protected "7urh" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:28, 27 April 2022

OCA: New page: '''Unreleased structure''' The entry 7urh is ON HOLD Authors: Description: Category: Unreleased Structures

2022-04-27T10:28:45Z

New page: '''Unreleased structure''' The entry 7urh is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7urh is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]