7ve6 - Revision history

OCA at 17:23, 29 November 2023

2023-11-29T17:23:29Z

←Older revision		Revision as of 17:23, 29 November 2023
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	==N-terminal domain of VraR==		==N-terminal domain of VraR==
-	<StructureSection load='7ve6' size='340' side='right'caption='[[7ve6]]' scene=''>	+	<StructureSection load='7ve6' size='340' side='right'caption='[[7ve6]], [[Resolution\|resolution]] 2.77Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VE6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VE6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7ve6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_Mu50 Staphylococcus aureus subsp. aureus Mu50]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VE6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VE6 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ve6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ve6 OCA], [https://pdbe.org/7ve6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ve6 RCSB], [https://www.ebi.ac.uk/pdbsum/7ve6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ve6 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.77Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BEF:BERYLLIUM+TRIFLUORIDE+ION'>BEF</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ve6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ve6 OCA], [https://pdbe.org/7ve6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ve6 RCSB], [https://www.ebi.ac.uk/pdbsum/7ve6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ve6 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/VRAR_STAAM VRAR_STAAM] Member of the two-component regulatory system VraS/VraR involved in the control of the cell wall peptidoglycan biosynthesis. VraR is overexpressed in strain Mu50, which leads to vancomycin resistance.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	In Staphylococcus aureus, vancomycin-resistance-associated response regulator (VraR) is a part of the VraSR two-component system, which is responsible for activating a cell wall-stress stimulon in response to an antibiotic that inhibits cell wall formation. Two VraR-binding sites have been identified: R1 and R2 in the vraSR operon control region. However, the binding of VraR to a promoter DNA enhancing downstream gene expression remains unclear. VraR contains a conserved N-terminal receiver domain (VraRN ) connected to a C-terminal DNA binding domain (VraRC ) with a flexible linker. Here, we present the crystal structure of VraRC alone and in complex with R1-DNA in 1.87- and 2.0-A resolution, respectively. VraRC consisting of four alpha-helices forms a dimer when interacting with R1-DNA. In the VraRC -DNA complex structure, Mg(2+) ion is bound to Asp194. Biolayer interferometry experiments revealed that the addition of Mg(2+) to VraRC enhanced its DNA binding affinity by eightfold. In addition, interpretation of NMR titrations between VraRC with R1- and R2-DNA revealed the essential residues that might play a crucial role in interacting with DNA of the vraSR operon. The structural information could help in designing and screening potential therapeutics/inhibitors to deal with antibiotic-resistant S. aureus via targeting VraR.
		+
		+	Structural insights into DNA binding domain of vancomycin-resistance-associated response regulator in complex with its promoter DNA from Staphylococcus aureus.,Kumar JV, Tseng TS, Lou YC, Wei SY, Wu TH, Tang HC, Chiu YC, Hsu CH, Chen C Protein Sci. 2022 May;31(5):e4286. doi: 10.1002/pro.4286. PMID:35481641<ref>PMID:35481641</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7ve6" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Response regulator 3D structure\|Response regulator 3D structure]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Staphylococcus aureus subsp. aureus Mu50]]
	[[Category: Chen C]]		[[Category: Chen C]]
	[[Category: Hsu CH]]		[[Category: Hsu CH]]
	[[Category: Kumar JV]]		[[Category: Kumar JV]]

OCA at 10:17, 18 May 2022

2022-05-18T10:17:35Z

←Older revision		Revision as of 10:17, 18 May 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7ve6 ~~is ON HOLD until Paper Publication~~	+	==N-terminal domain of VraR==
-		+	<StructureSection load='7ve6' size='340' side='right'caption='[[7ve6]]' scene=''>
-	~~Authors~~: ~~Kumar~~, J.V., ~~Chen~~, C., ~~Hsu~~, C.H.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VE6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VE6 FirstGlance]. <br>
-	~~Description: N-terminal domain of VraR~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ve6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ve6 OCA], [https://pdbe.org/7ve6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ve6 RCSB], [https://www.ebi.ac.uk/pdbsum/7ve6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ve6 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Kumar, J.V~~]]	+	__TOC__
-	[[Category: Hsu~~, C.H~~]]	+	</StructureSection>
-	[[Category: ~~Chen, C~~]]	+	[[Category: Large Structures]]
		+	[[Category: Chen C]]
		+	[[Category: Hsu CH]]
		+	[[Category: Kumar JV]]

OCA at 04:20, 6 October 2021

2021-10-06T04:20:37Z

←Older revision		Revision as of 04:20, 6 October 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7ve6 is ON HOLD	+	The entry 7ve6 is ON HOLD until Paper Publication

	Authors: Kumar, J.V., Chen, C., Hsu, C.H.		Authors: Kumar, J.V., Chen, C., Hsu, C.H.

OCA: Protected "7ve6" [edit=sysop:move=sysop]

2021-09-15T08:28:24Z

Protected "7ve6" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:28, 15 September 2021

OCA: New page: '''Unreleased structure''' The entry 7ve6 is ON HOLD Authors: Kumar, J.V., Chen, C., Hsu, C.H. Description: N-terminal domain of VraR Category: Unreleased Structures [[Category: Ku...

2021-09-15T08:28:23Z

New page: '''Unreleased structure''' The entry 7ve6 is ON HOLD Authors: Kumar, J.V., Chen, C., Hsu, C.H. Description: N-terminal domain of VraR Category: Unreleased Structures [[Category: Ku...

New page

'''Unreleased structure'''

The entry 7ve6 is ON HOLD

Authors: Kumar, J.V., Chen, C., Hsu, C.H.

Description: N-terminal domain of VraR
[[Category: Unreleased Structures]]
[[Category: Kumar, J.V]]
[[Category: Hsu, C.H]]
[[Category: Chen, C]]