OCA at 10:24, 13 April 2022

2022-04-13T10:24:36Z

←Older revision		Revision as of 10:24, 13 April 2022
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	==Cryo-EM structure of Vaccinia virus scaffolding protein D13 with N-terminal polyhistidine tag==		==Cryo-EM structure of Vaccinia virus scaffolding protein D13 with N-terminal polyhistidine tag==
-	<StructureSection load='7vfe' size='340' side='right'caption='[[7vfe]]' scene=''>	+	<StructureSection load='7vfe' size='340' side='right'caption='[[7vfe]], [[Resolution\|resolution]] 2.63Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VFE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VFE FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7vfe]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VFE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VFE FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vfe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vfe OCA], [https://pdbe.org/7vfe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vfe RCSB], [https://www.ebi.ac.uk/pdbsum/7vfe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vfe ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vfe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vfe OCA], [https://pdbe.org/7vfe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vfe RCSB], [https://www.ebi.ac.uk/pdbsum/7vfe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vfe ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/D13_VACCW D13_VACCW]] Scaffold protein which forms a transitory spherical honeycomb lattice providing curvature and rigidity to the convex membrane of crescent and immature virions (IV). This association occurs concomitantly with viral membrane formation. Targeted by the drug rifampicin, which prevents the formation of this lattice, and hence virus morphogenesis. In the presence of rifampicin, irregularly shaped membranes that lack the honeycomb layer accumulate around areas of electron-dense viroplasm. This layer is lost from virions during maturation from IV to mature virion (MV), through the proteolysis of A17 N-terminus.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	In Vaccinia virus (VACV), the prototype poxvirus, scaffold protein D13 forms a honeycomb-like lattice on the viral membrane that results in formation of the pleomorphic immature virion (IV). The structure of D13 is similar to those of major capsid proteins that readily form icosahedral capsids in nucleocytoplasmic large DNA viruses (NCLDVs). However, the detailed assembly mechanism of the nonicosahedral poxvirus scaffold has never been understood. Here we show the cryo-EM structures of the D13 trimer and scaffold intermediates produced in vitro. The structures reveal that the displacement of the short N-terminal alpha-helix is critical for initiation of D13 self-assembly. The continuous curvature of the IV is mediated by electrostatic interactions that induce torsion between trimers. The assembly mechanism explains the semiordered capsid-like arrangement of D13 that is distinct from icosahedral NCLDVs. Our structures explain how a single protein can self-assemble into different capsid morphologies and represent a local exception to the universal Caspar-Klug theory of quasi-equivalence.
		+
		+	Assembly mechanism of the pleomorphic immature poxvirus scaffold.,Hyun J, Matsunami H, Kim TG, Wolf M Nat Commun. 2022 Mar 31;13(1):1704. doi: 10.1038/s41467-022-29305-5. PMID:35361762<ref>PMID:35361762</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7vfe" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Hyun J]]	+	[[Category: Hyun, J]]
-	[[Category: Kim TG]]	+	[[Category: Kim, T G]]
-	[[Category: Matsunami H]]	+	[[Category: Matsunami, H]]
-	[[Category: Wolf M]]	+	[[Category: Wolf, M]]
		+	[[Category: Capsid]]
		+	[[Category: Double-jelly-roll]]
		+	[[Category: Scaffold]]
		+	[[Category: Viral protein]]

OCA at 07:45, 23 February 2022

2022-02-23T07:45:13Z

←Older revision		Revision as of 07:45, 23 February 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7vfe is ~~ON HOLD until Paper Publication~~	+	==Cryo-EM structure of Vaccinia virus scaffolding protein D13 with N-terminal polyhistidine tag==
-		+	<StructureSection load='7vfe' size='340' side='right'caption='[[7vfe]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VFE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VFE FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vfe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vfe OCA], [https://pdbe.org/7vfe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vfe RCSB], [https://www.ebi.ac.uk/pdbsum/7vfe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vfe ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Hyun J]]
		+	[[Category: Kim TG]]
		+	[[Category: Matsunami H]]
		+	[[Category: Wolf M]]

OCA at 12:20, 29 December 2021

2021-12-29T12:20:22Z

←Older revision		Revision as of 12:20, 29 December 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7vfe is ON HOLD	+	The entry 7vfe is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7vfe" [edit=sysop:move=sysop]

2021-09-22T05:43:57Z

Protected "7vfe" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:43, 22 September 2021

OCA: New page: '''Unreleased structure''' The entry 7vfe is ON HOLD Authors: Description: Category: Unreleased Structures

2021-09-22T05:43:56Z

New page: '''Unreleased structure''' The entry 7vfe is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7vfe is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7vfe - Revision history

OCA at 10:24, 13 April 2022

OCA at 07:45, 23 February 2022

OCA at 12:20, 29 December 2021

OCA: Protected "7vfe" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7vfe is ON HOLD Authors: Description: Category: Unreleased Structures