7vgm - Revision history

OCA at 19:42, 29 May 2024

2024-05-29T19:42:52Z

←Older revision		Revision as of 19:42, 29 May 2024
Line 3:		Line 3:
	<StructureSection load='7vgm' size='340' side='right'caption='[[7vgm]], [[Resolution\|resolution]] 2.27Å' scene=''>		<StructureSection load='7vgm' size='340' side='right'caption='[[7vgm]], [[Resolution\|resolution]] 2.27Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>~~[[7vgm]] is a 1 chain structure.~~ Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VGM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VGM FirstGlance]. <br>	+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VGM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VGM FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.27Å</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Phenylalanine_4-monooxygenase Phenylalanine 4-monooxygenase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.16.1 1.14.16.1] </span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vgm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vgm OCA], [https://pdbe.org/7vgm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vgm RCSB], [https://www.ebi.ac.uk/pdbsum/7vgm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vgm ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vgm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vgm OCA], [https://pdbe.org/7vgm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vgm RCSB], [https://www.ebi.ac.uk/pdbsum/7vgm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vgm ProSAT]</span></td></tr>
	</table>		</table>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Phenylalanine hydroxylase (PAH), which belongs to the aromatic amino-acid hydroxylase family, is involved in protein synthesis and pyomelanine production through the hydroxylation of phenylalanine to tyrosine. In this study, the crystal structure of PAH from Bacillus cereus ATCC 14579 (BcPAH) with an additional 280 amino acids in the C-terminal region was determined. The structure of BcPAH consists of three distinct domains: a core domain with two additional inserted alpha-helices and two novel auxiliary domains: BcPAH-AD1 and BcPAH-AD2. Structural homologues of BcPAH-AD1 and BcPAH-AD2 are known to be involved in mRNA regulation and protein-protein interactions, and thus it was speculated that BcPAH might utilize the auxiliary domains for interaction with its partner proteins. Furthermore, phylogenetic tree analysis revealed that the three-domain PAHs, including BcPAH, are completely distinctive from both conventional prokaryotic PAHs and eukaryotic PAHs. Finally, biochemical studies of BcPAH showed that BcPAH-AD1 might be important for the structural integrity of the enzyme and that BcPAH-AD2 is related to enzyme stability and/or activity. Investigations into the intracellular functions of the two auxiliary domains and the relationship between these functions and the activity of PAH are required.

-	Structural studies of a novel auxiliary-domain-containing phenylalanine hydroxylase from Bacillus cereus ATCC 14579.,Park J, Hong J, Seok J, Hong H, Seo H, Kim KJ Acta Crystallogr D Struct Biol. 2022 May 1;78(Pt 5):586-598. doi:, 10.1107/S2059798322002674. Epub 2022 Apr 8. PMID:35503207<ref>PMID:35503207</ref>	+	==See Also==
-		+	*[[Hydroxylases 3D structures\|Hydroxylases 3D structures]]
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 7vgm" style="background-color:#fffaf0;"></div>~~	+
-	== ~~References~~ ==	+
-	~~<references/>~~	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Phenylalanine 4-monooxygenase]]~~	+	[[Category: Kim K-J]]
-	[[Category: Kim, K J]]	+	[[Category: Park J]]
-	[[Category: Park, J]]	+
-	~~[[Category: Metal binding protein]]~~	+
-	~~[[Category: Phenylalanine hydroxylase~~]]	+

OCA at 10:28, 18 May 2022

2022-05-18T10:28:42Z

←Older revision		Revision as of 10:28, 18 May 2022
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	==Crystal structure of Phenylalanine hydroxylase from Bacillus cereus ATCC 14579==		==Crystal structure of Phenylalanine hydroxylase from Bacillus cereus ATCC 14579==
-	<StructureSection load='7vgm' size='340' side='right'caption='[[7vgm]]' scene=''>	+	<StructureSection load='7vgm' size='340' side='right'caption='[[7vgm]], [[Resolution\|resolution]] 2.27Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VGM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VGM FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7vgm]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VGM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VGM FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vgm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vgm OCA], [https://pdbe.org/7vgm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vgm RCSB], [https://www.ebi.ac.uk/pdbsum/7vgm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vgm ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACY:ACETIC+ACID'>ACY</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Phenylalanine_4-monooxygenase Phenylalanine 4-monooxygenase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.16.1 1.14.16.1] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vgm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vgm OCA], [https://pdbe.org/7vgm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vgm RCSB], [https://www.ebi.ac.uk/pdbsum/7vgm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vgm ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Phenylalanine hydroxylase (PAH), which belongs to the aromatic amino-acid hydroxylase family, is involved in protein synthesis and pyomelanine production through the hydroxylation of phenylalanine to tyrosine. In this study, the crystal structure of PAH from Bacillus cereus ATCC 14579 (BcPAH) with an additional 280 amino acids in the C-terminal region was determined. The structure of BcPAH consists of three distinct domains: a core domain with two additional inserted alpha-helices and two novel auxiliary domains: BcPAH-AD1 and BcPAH-AD2. Structural homologues of BcPAH-AD1 and BcPAH-AD2 are known to be involved in mRNA regulation and protein-protein interactions, and thus it was speculated that BcPAH might utilize the auxiliary domains for interaction with its partner proteins. Furthermore, phylogenetic tree analysis revealed that the three-domain PAHs, including BcPAH, are completely distinctive from both conventional prokaryotic PAHs and eukaryotic PAHs. Finally, biochemical studies of BcPAH showed that BcPAH-AD1 might be important for the structural integrity of the enzyme and that BcPAH-AD2 is related to enzyme stability and/or activity. Investigations into the intracellular functions of the two auxiliary domains and the relationship between these functions and the activity of PAH are required.
		+
		+	Structural studies of a novel auxiliary-domain-containing phenylalanine hydroxylase from Bacillus cereus ATCC 14579.,Park J, Hong J, Seok J, Hong H, Seo H, Kim KJ Acta Crystallogr D Struct Biol. 2022 May 1;78(Pt 5):586-598. doi:, 10.1107/S2059798322002674. Epub 2022 Apr 8. PMID:35503207<ref>PMID:35503207</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7vgm" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Kim K-J]]	+	[[Category: Phenylalanine 4-monooxygenase]]
-	[[Category: Park J]]	+	[[Category: Kim, K J]]
		+	[[Category: Park, J]]
		+	[[Category: Metal binding protein]]
		+	[[Category: Phenylalanine hydroxylase]]

OCA at 11:39, 27 April 2022

2022-04-27T11:39:58Z

←Older revision		Revision as of 11:39, 27 April 2022
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7vgm ~~is ON HOLD until Paper Publication~~	+	==Crystal structure of Phenylalanine hydroxylase from Bacillus cereus ATCC 14579==
-		+	<StructureSection load='7vgm' size='340' side='right'caption='[[7vgm]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VGM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VGM FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vgm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vgm OCA], [https://pdbe.org/7vgm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vgm RCSB], [https://www.ebi.ac.uk/pdbsum/7vgm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vgm ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Kim K-J]]
		+	[[Category: Park J]]

OCA at 07:52, 29 September 2021

2021-09-29T07:52:48Z

←Older revision		Revision as of 07:52, 29 September 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7vgm is ON HOLD	+	The entry 7vgm is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7vgm" [edit=sysop:move=sysop]

2021-09-22T05:46:10Z

Protected "7vgm" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:46, 22 September 2021

OCA: New page: '''Unreleased structure''' The entry 7vgm is ON HOLD Authors: Description: Category: Unreleased Structures

2021-09-22T05:46:09Z

New page: '''Unreleased structure''' The entry 7vgm is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7vgm is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]