7vjo - Revision history

OCA at 17:27, 29 November 2023

2023-11-29T17:27:00Z

←Older revision		Revision as of 17:27, 29 November 2023
Line 3:		Line 3:
	<StructureSection load='7vjo' size='340' side='right'caption='[[7vjo]], [[Resolution\|resolution]] 1.31Å' scene=''>		<StructureSection load='7vjo' size='340' side='right'caption='[[7vjo]], [[Resolution\|resolution]] 1.31Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7vjo]] is a 2 chain structure. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=7cq8 7cq8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VJO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VJO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7vjo]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pectobacterium_phage_ZF40 Pectobacterium phage ZF40]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=7cq8 7cq8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VJO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VJO FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.31Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vjo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vjo OCA], [https://pdbe.org/7vjo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vjo RCSB], [https://www.ebi.ac.uk/pdbsum/7vjo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vjo ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vjo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vjo OCA], [https://pdbe.org/7vjo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vjo RCSB], [https://www.ebi.ac.uk/pdbsum/7vjo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vjo ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/H9C180_9CAUD H9C180_9CAUD]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 24:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Huang, H D~~]]	+	[[Category: Pectobacterium phage ZF40]]
-	[[Category: ~~Liu, Y H~~]]	+	[[Category: Huang HD]]
-	[[Category: ~~Wu, B X~~]]	+	[[Category: Liu YH]]
-	[[Category: ~~Zhang, L S~~]]	+	[[Category: Wu BX]]
-	[[Category: ~~Aca2]]~~	+	[[Category: Zhang LS]]
-	~~[[Category: Anti-crispr]]~~	+
-	~~[[Category: Anti-crispr-associated protein]]~~	+
-	~~[[Category: Crispr]]~~	+
-	~~[[Category: Repression]]~~	+
-	~~[[Category: Transcription~~]]	+

OCA at 07:18, 2 February 2022

2022-02-02T07:18:32Z

←Older revision		Revision as of 07:18, 2 February 2022
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	==Pectobacterium phage ZF40 apo-Aca2==		==Pectobacterium phage ZF40 apo-Aca2==
-	<StructureSection load='7vjo' size='340' side='right'caption='[[7vjo]]' scene=''>	+	<StructureSection load='7vjo' size='340' side='right'caption='[[7vjo]], [[Resolution\|resolution]] 1.31Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=7cq8 7cq8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VJO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VJO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7vjo]] is a 2 chain structure. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=7cq8 7cq8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VJO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VJO FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vjo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vjo OCA], [https://pdbe.org/7vjo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vjo RCSB], [https://www.ebi.ac.uk/pdbsum/7vjo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vjo ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vjo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vjo OCA], [https://pdbe.org/7vjo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vjo RCSB], [https://www.ebi.ac.uk/pdbsum/7vjo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vjo ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	It has been shown that phages have evolved anti-CRISPR (Acr) proteins to inhibit host CRISPR-Cas systems. Most acr genes are located upstream of anti-CRISPR-associated (aca) genes, which is instrumental for identifying these acr genes. Thus far, eight Aca families (Aca1-Aca8) have been identified, all proteins of which share low sequence homology and bind to different target DNA sequences. Recently, Aca1 and Aca2 proteins were discovered to function as repressors by binding to acr-aca promoters, thus implying a potential anti-anti-CRISPR mechanism. However, the structural basis for the repression roles of Aca proteins is still unknown. Here, we elucidated apo-structures of Aca1 and Aca2 proteins and their complex structures with their cognate operator DNA in two model systems, the Pseudomonas phage JBD30 and the Pectobacterium carotovorum template phage ZF40. In combination with biochemical and cellular assays, our study unveils dimerization and DNA-recognition mechanisms of Aca1 and Aca2 family proteins, thus revealing the molecular basis for Aca1-and Aca2-mediated anti-CRISPR repression. Our results also shed light on understanding the repression roles of other Aca family proteins and autoregulation roles of acr-aca operons.
		+
		+	Structural basis for anti-CRISPR repression mediated by bacterial operon proteins Aca1 and Aca2.,Liu Y, Zhang L, Guo M, Chen L, Wu B, Huang H J Biol Chem. 2021 Dec;297(6):101357. doi: 10.1016/j.jbc.2021.101357. Epub 2021, Oct 28. PMID:34756887<ref>PMID:34756887</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7vjo" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Huang HD]]	+	[[Category: Huang, H D]]
-	[[Category: Liu YH]]	+	[[Category: Liu, Y H]]
-	[[Category: Wu BX]]	+	[[Category: Wu, B X]]
-	[[Category: Zhang LS]]	+	[[Category: Zhang, L S]]
		+	[[Category: Aca2]]
		+	[[Category: Anti-crispr]]
		+	[[Category: Anti-crispr-associated protein]]
		+	[[Category: Crispr]]
		+	[[Category: Repression]]
		+	[[Category: Transcription]]

OCA at 16:25, 20 October 2021

2021-10-20T16:25:08Z

←Older revision		Revision as of 16:25, 20 October 2021
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7vjo is ~~ON HOLD~~	+	==Pectobacterium phage ZF40 apo-Aca2==
-		+	<StructureSection load='7vjo' size='340' side='right'caption='[[7vjo]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=7cq8 7cq8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VJO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VJO FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vjo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vjo OCA], [https://pdbe.org/7vjo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vjo RCSB], [https://www.ebi.ac.uk/pdbsum/7vjo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vjo ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Huang HD]]
		+	[[Category: Liu YH]]
		+	[[Category: Wu BX]]
		+	[[Category: Zhang LS]]

OCA: Protected "7vjo" [edit=sysop:move=sysop]

2021-10-06T04:24:12Z

Protected "7vjo" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:24, 6 October 2021

OCA: New page: '''Unreleased structure''' The entry 7vjo is ON HOLD Authors: Description: Category: Unreleased Structures

2021-10-06T04:24:11Z

New page: '''Unreleased structure''' The entry 7vjo is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7vjo is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]