7vpa - Revision history

OCA at 17:29, 29 November 2023

2023-11-29T17:29:40Z

←Older revision		Revision as of 17:29, 29 November 2023
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	==Crystal structure of Ple629 from marine microbial consortium==		==Crystal structure of Ple629 from marine microbial consortium==
-	<StructureSection load='7vpa' size='340' side='right'caption='[[7vpa]]' scene=''>	+	<StructureSection load='7vpa' size='340' side='right'caption='[[7vpa]], [[Resolution\|resolution]] 2.35Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VPA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VPA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7vpa]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Unclassified_Marinobacter Unclassified Marinobacter]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VPA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VPA FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vpa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vpa OCA], [https://pdbe.org/7vpa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vpa RCSB], [https://www.ebi.ac.uk/pdbsum/7vpa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vpa ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.35Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vpa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vpa OCA], [https://pdbe.org/7vpa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vpa RCSB], [https://www.ebi.ac.uk/pdbsum/7vpa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vpa ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Polybutylene adipate terephthalate (PBAT) is a biodegradable alternative to polyethylene and can be broadly used in various applications. These polymers can be degraded by hydrolases of terrestrial and aquatic origin. In a previous study, we identified tandem PETase-like hydrolases (Ples) from the marine microbial consortium I1 that were highly expressed when a PBAT blend was supplied as the only carbon source. In this study, the tandem Ples, Ple628 and Ple629, were recombinantly expressed and characterized. Both enzymes are mesophilic and active on a wide range of oligomers. The activities of the Ples differed greatly when model substrates, PBAT-modified polymers or PET nanoparticles were supplied. Ple629 was always more active than Ple628. Crystal structures of Ple628 and Ple629 revealed a structural similarity to other PETases and can be classified as member of the PETases IIa subclass, alpha/beta hydrolase superfamily. Our results show that the predicted functions of Ple628 and Ple629 agree with the bioinformatic predictions, and these enzymes play a significant role in the plastic degradation by the consortium.
		+
		+	Molecular and Biochemical Differences of the Tandem and Cold-Adapted PET Hydrolases Ple628 and Ple629, Isolated From a Marine Microbial Consortium.,Meyer Cifuentes IE, Wu P, Zhao Y, Liu W, Neumann-Schaal M, Pfaff L, Barys J, Li Z, Gao J, Han X, Bornscheuer UT, Wei R, Ozturk B Front Bioeng Biotechnol. 2022 Jul 21;10:930140. doi: 10.3389/fbioe.2022.930140., eCollection 2022. PMID:35935485<ref>PMID:35935485</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7vpa" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Unclassified Marinobacter]]
	[[Category: Basak O]]		[[Category: Basak O]]
	[[Category: Gao J]]		[[Category: Gao J]]

OCA at 04:51, 25 August 2022

2022-08-25T04:51:33Z

←Older revision		Revision as of 04:51, 25 August 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7vpa is ~~ON HOLD until Paper Publication~~	+	==Crystal structure of Ple629 from marine microbial consortium==
-		+	<StructureSection load='7vpa' size='340' side='right'caption='[[7vpa]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VPA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VPA FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vpa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vpa OCA], [https://pdbe.org/7vpa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vpa RCSB], [https://www.ebi.ac.uk/pdbsum/7vpa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vpa ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Basak O]]
		+	[[Category: Gao J]]
		+	[[Category: Han X]]
		+	[[Category: Ingrid MC]]
		+	[[Category: Lara P]]
		+	[[Category: Li Q]]
		+	[[Category: Li ZS]]
		+	[[Category: Liu WD]]
		+	[[Category: Wei R]]
		+	[[Category: Wu P]]
		+	[[Category: Zhao YP]]

OCA at 12:26, 29 December 2021

2021-12-29T12:26:27Z

←Older revision		Revision as of 12:26, 29 December 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7vpa is ON HOLD	+	The entry 7vpa is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7vpa" [edit=sysop:move=sysop]

2021-10-20T15:19:51Z

Protected "7vpa" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:19, 20 October 2021

OCA: New page: '''Unreleased structure''' The entry 7vpa is ON HOLD Authors: Description: Category: Unreleased Structures

2021-10-20T15:19:50Z

New page: '''Unreleased structure''' The entry 7vpa is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7vpa is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]