7vr8 - Revision history

OCA at 06:29, 28 September 2022

2022-09-28T06:29:49Z

←Older revision		Revision as of 06:29, 28 September 2022
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	==Inward-facing structure of human EAAT2 in the substrate-free state==		==Inward-facing structure of human EAAT2 in the substrate-free state==
-	<StructureSection load='7vr8' size='340' side='right'caption='[[7vr8]]' scene=''>	+	<StructureSection load='7vr8' size='340' side='right'caption='[[7vr8]], [[Resolution\|resolution]] 3.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VR8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VR8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7vr8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VR8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VR8 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vr8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vr8 OCA], [https://pdbe.org/7vr8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vr8 RCSB], [https://www.ebi.ac.uk/pdbsum/7vr8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vr8 ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=9Z9:(3beta,14beta,17beta,25R)-3-[4-methoxy-3-(methoxymethyl)butoxy]spirost-5-en'>9Z9</scene>, <scene name='pdbligand=CLR:CHOLESTEROL'>CLR</scene>, <scene name='pdbligand=PC1:1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE'>PC1</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vr8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vr8 OCA], [https://pdbe.org/7vr8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vr8 RCSB], [https://www.ebi.ac.uk/pdbsum/7vr8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vr8 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Disease ==
		+	[[https://www.uniprot.org/uniprot/EAA2_HUMAN EAA2_HUMAN]] Non-specific early-onset epileptic encephalopathy. The disease is caused by variants affecting the gene represented in this entry.
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/EAA2_HUMAN EAA2_HUMAN]] Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate (PubMed:7521911, PubMed:14506254, PubMed:15265858, PubMed:26690923). Functions as a symporter that transports one amino acid molecule together with two or three Na(+) ions and one proton, in parallel with the counter-transport of one K(+) ion (PubMed:14506254). Mediates Cl(-) flux that is not coupled to amino acid transport; this avoids the accumulation of negative charges due to aspartate and Na(+) symport (PubMed:14506254). Essential for the rapid removal of released glutamate from the synaptic cleft, and for terminating the postsynaptic action of glutamate (By similarity).[UniProtKB:P43006]<ref>PMID:15265858</ref> <ref>PMID:26690923</ref> <ref>PMID:7521911</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Glutamate is a pivotal excitatory neurotransmitter in mammalian brains, but excessive glutamate causes numerous neural disorders. Almost all extracellular glutamate is retrieved by the glial transporter, Excitatory Amino Acid Transporter 2 (EAAT2), belonging to the SLC1A family. However, in some cancers, EAAT2 expression is enhanced and causes resistance to therapies by metabolic disturbance. Despite its crucial roles, the detailed structural information about EAAT2 has not been available. Here, we report cryo-EM structures of human EAAT2 in substrate-free and selective inhibitor WAY213613-bound states at 3.2 A and 2.8 A, respectively. EAAT2 forms a trimer, with each protomer consisting of transport and scaffold domains. Along with a glutamate-binding site, the transport domain possesses a cavity that could be disrupted during the transport cycle. WAY213613 occupies both the glutamate-binding site and cavity of EAAT2 to interfere with its alternating access, where the sensitivity is defined by the inner environment of the cavity. We provide the characterization of the molecular features of EAAT2 and its selective inhibition mechanism that may facilitate structure-based drug design for EAAT2.
		+
		+	Structural insights into inhibitory mechanism of human excitatory amino acid transporter EAAT2.,Kato T, Kusakizako T, Jin C, Zhou X, Ohgaki R, Quan L, Xu M, Okuda S, Kobayashi K, Yamashita K, Nishizawa T, Kanai Y, Nureki O Nat Commun. 2022 Aug 11;13(1):4714. doi: 10.1038/s41467-022-32442-6. PMID:35953475<ref>PMID:35953475</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7vr8" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Kato T]]		[[Category: Kato T]]

OCA at 05:01, 10 August 2022

2022-08-10T05:01:37Z

←Older revision		Revision as of 05:01, 10 August 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7vr8 is ~~ON HOLD until Paper Publication~~	+	==Inward-facing structure of human EAAT2 in the substrate-free state==
-		+	<StructureSection load='7vr8' size='340' side='right'caption='[[7vr8]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VR8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VR8 FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vr8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vr8 OCA], [https://pdbe.org/7vr8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vr8 RCSB], [https://www.ebi.ac.uk/pdbsum/7vr8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vr8 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Kato T]]
		+	[[Category: Kusakizako T]]
		+	[[Category: Nishizawa T]]
		+	[[Category: Nureki O]]
		+	[[Category: Yamashita K]]

OCA at 03:53, 15 June 2022

2022-06-15T03:53:12Z

←Older revision		Revision as of 03:53, 15 June 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7vr8 is ON HOLD	+	The entry 7vr8 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA at 06:42, 25 May 2022

2022-05-25T06:42:21Z

←Older revision		Revision as of 06:42, 25 May 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7vr8 is ON HOLD ~~until Paper Publication~~	+	The entry 7vr8 is ON HOLD

	Authors:		Authors:

OCA at 13:00, 3 November 2021

2021-11-03T13:00:35Z

←Older revision		Revision as of 13:00, 3 November 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7vr8 is ON HOLD	+	The entry 7vr8 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7vr8" [edit=sysop:move=sysop]

2021-10-27T12:54:12Z

Protected "7vr8" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:54, 27 October 2021

OCA: New page: '''Unreleased structure''' The entry 7vr8 is ON HOLD Authors: Description: Category: Unreleased Structures

2021-10-27T12:54:11Z

New page: '''Unreleased structure''' The entry 7vr8 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7vr8 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]