7vrg - Revision history

OCA at 17:31, 29 November 2023

2023-11-29T17:31:05Z

←Older revision		Revision as of 17:31, 29 November 2023
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	==Crystal structure of chitinase-h from O. furnacalis in complex with Lynamicin B==		==Crystal structure of chitinase-h from O. furnacalis in complex with Lynamicin B==
-	<StructureSection load='7vrg' size='340' side='right'caption='[[7vrg]]' scene=''>	+	<StructureSection load='7vrg' size='340' side='right'caption='[[7vrg]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VRG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VRG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7vrg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Ostrinia_furnacalis Ostrinia furnacalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VRG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VRG FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vrg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vrg OCA], [https://pdbe.org/7vrg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vrg RCSB], [https://www.ebi.ac.uk/pdbsum/7vrg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vrg ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.4Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=7U8:methyl+4-[5,6-bis(chloranyl)-1H-indol-3-yl]-3-(5-chloranyl-1H-indol-3-yl)-1H-pyrrole-2-carboxylate'>7U8</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vrg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vrg OCA], [https://pdbe.org/7vrg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vrg RCSB], [https://www.ebi.ac.uk/pdbsum/7vrg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vrg ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q4AE59_OSTFU Q4AE59_OSTFU]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Insect group h chitinase is a promising target for designing non-target safe pesticides in that it is exclusively distributed in lepidopteran insects, over 80% of which are agricultural pests. In this work, lynamicin B was discovered to be an inhibitor of OfChi-h, the group h chitinase from the lepidopteran pest Ostrinia furnacalis. Lynamicin B was revealed to competitively inhibit OfChi-h with a Ki value of 8.76 muM and does not significantly inhibit other chitinases. The co-crystal structure of lynamicin B and OfChi-h revealed that the dichloroindolyl group of lynamicin B occupies an unexplored pocket below subsites +1 and +2 of the substrate-binding cleft, which is vital for its selectivity. Feeding experiments demonstrated that lynamicin B exhibited high insecticidal activities against other lepidopteran pests Mythimna separata and Spodoptera frugiperda besides O. furnacalis. Moreover, lynamicin B did not affect Trichogramma ostriniae, a natural enemy of O. furnacalis. This study provides a natural-derived potent pesticide for the control of lepidopteran pests, leaving its natural enemy unaffected.
		+
		+	Lynamicin B is a Potential Pesticide by Acting as a Lepidoptera-Exclusive Chitinase Inhibitor.,Lu Q, Xu L, Liu L, Zhou Y, Liu T, Song Y, Ju J, Yang Q J Agric Food Chem. 2021 Dec 1;69(47):14086-14091. doi: 10.1021/acs.jafc.1c05385. , Epub 2021 Nov 19. PMID:34797675<ref>PMID:34797675</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7vrg" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Chitinase 3D structures\|Chitinase 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Ostrinia furnacalis]]
	[[Category: Liu T]]		[[Category: Liu T]]
	[[Category: Lu Q]]		[[Category: Lu Q]]
	[[Category: Yang Q]]		[[Category: Yang Q]]
	[[Category: Zhou Y]]		[[Category: Zhou Y]]

OCA at 06:23, 2 March 2022

2022-03-02T06:23:34Z

←Older revision		Revision as of 06:23, 2 March 2022
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-	'''Unreleased structure'''

-	~~The entry 7vrg is ON HOLD~~	+	==Crystal structure of chitinase-h from O. furnacalis in complex with Lynamicin B==
-		+	<StructureSection load='7vrg' size='340' side='right'caption='[[7vrg]]' scene=''>
-	~~Authors: Lu, Q., Liu, T., Zhou, Y., Yang, Q.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VRG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VRG FirstGlance]. <br>
-	~~Description:~~ Crystal structure of chitinase-h from O. furnacalis in complex with Lynamicin B	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vrg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vrg OCA], [https://pdbe.org/7vrg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vrg RCSB], [https://www.ebi.ac.uk/pdbsum/7vrg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vrg ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Yang, Q~~]]	+	__TOC__
-	[[Category: ~~Zhou, Y~~]]	+	</StructureSection>
-	[[Category: ~~Lu,~~ Q]]	+	[[Category: Large Structures]]
-	[[Category: ~~Liu, T~~]]	+	[[Category: Liu T]]
		+	[[Category: Lu Q]]
		+	[[Category: Yang Q]]
		+	[[Category: Zhou Y]]

OCA: Protected "7vrg" [edit=sysop:move=sysop]

2021-11-03T13:00:41Z

Protected "7vrg" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:00, 3 November 2021

OCA: New page: '''Unreleased structure''' The entry 7vrg is ON HOLD Authors: Lu, Q., Liu, T., Zhou, Y., Yang, Q. Description: Crystal structure of chitinase-h from O. furnacalis in complex with Lynam...

2021-11-03T13:00:40Z

New page: '''Unreleased structure''' The entry 7vrg is ON HOLD Authors: Lu, Q., Liu, T., Zhou, Y., Yang, Q. Description: Crystal structure of chitinase-h from O. furnacalis in complex with Lynam...

New page

'''Unreleased structure'''

The entry 7vrg is ON HOLD

Authors: Lu, Q., Liu, T., Zhou, Y., Yang, Q.

Description: Crystal structure of chitinase-h from O. furnacalis in complex with Lynamicin B
[[Category: Unreleased Structures]]
[[Category: Yang, Q]]
[[Category: Zhou, Y]]
[[Category: Lu, Q]]
[[Category: Liu, T]]