OCA at 11:18, 30 March 2022

2022-03-30T11:18:56Z

←Older revision		Revision as of 11:18, 30 March 2022
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	==Crystal strcuture of the tandem B-Box domains of COL2==		==Crystal strcuture of the tandem B-Box domains of COL2==
-	<StructureSection load='7vsp' size='340' side='right'caption='[[7vsp]]' scene=''>	+	<StructureSection load='7vsp' size='340' side='right'caption='[[7vsp]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VSP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VSP FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7vsp]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VSP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VSP FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vsp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vsp OCA], [https://pdbe.org/7vsp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vsp RCSB], [https://www.ebi.ac.uk/pdbsum/7vsp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vsp ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vsp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vsp OCA], [https://pdbe.org/7vsp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vsp RCSB], [https://www.ebi.ac.uk/pdbsum/7vsp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vsp ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/COL2_ARATH COL2_ARATH]] Putative transcription factor. Does not affect flowering time.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The transcription factor CONSTANS (CO) integrates day-length information to induce the expression of florigen FLOWERING LOCUS T (FT) in Arabidopsis. We recently reported that the C-terminal CCT domain of CO forms a complex with NUCLEAR FACTOR-YB/YC to recognize multiple cis-elements in the FT promoter, and the N-terminal tandem B-box domains form a homomultimeric assembly. However, the mechanism and biological function of CO multimerization remained unclear. Here, we report that CO takes on a head-to-tail oligomeric configuration via its B-boxes to mediate FT activation in long days. The crystal structure of B-boxes(CO) reveals a closely connected tandem B-box fold forming a continuous head-to-tail assembly through unique CDHH zinc fingers. Mutating the key residues involved in CO oligomerization resulted in a non-functional CO, as evidenced by the inability to rescue co mutants. By contrast, a transgene encoding a human p53-derived tetrameric peptide in place of the B-boxes(CO) rescued co mutant, emphasizing the essential role of B-boxes(CO) -mediated oligomerization. Furthermore, we found that the four TGTG-bearing cis-elements in FT proximal promoter are required for FT activation in long days. Our results suggest that CO forms a multimer to bind to the four TGTG motifs in the FT promoter to mediate FT activation.
		+
		+	Molecular basis of CONSTANS oligomerization in FLOWERING LOCUS T activation.,Zeng X, Lv X, Liu R, He H, Liang S, Chen L, Zhang F, Chen L, He Y, Du J J Integr Plant Biol. 2022 Jan 12. doi: 10.1111/jipb.13223. PMID:35023269<ref>PMID:35023269</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7vsp" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Du J]]	+	[[Category: Du, J]]
-	[[Category: Liu R]]	+	[[Category: Liu, R]]
-	[[Category: Lv X]]	+	[[Category: Lv, X]]
		+	[[Category: B-box]]
		+	[[Category: Col2]]
		+	[[Category: Photoperiod]]
		+	[[Category: Plant protein]]

OCA at 06:23, 2 March 2022

2022-03-02T06:23:42Z

←Older revision		Revision as of 06:23, 2 March 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7vsp is ~~ON HOLD until Paper Publication~~	+	==Crystal strcuture of the tandem B-Box domains of COL2==
-		+	<StructureSection load='7vsp' size='340' side='right'caption='[[7vsp]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7VSP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7VSP FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7vsp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7vsp OCA], [https://pdbe.org/7vsp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7vsp RCSB], [https://www.ebi.ac.uk/pdbsum/7vsp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7vsp ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Du J]]
		+	[[Category: Liu R]]
		+	[[Category: Lv X]]

OCA at 12:28, 29 December 2021

2021-12-29T12:28:51Z

←Older revision		Revision as of 12:28, 29 December 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7vsp is ON HOLD	+	The entry 7vsp is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7vsp" [edit=sysop:move=sysop]

2021-11-03T13:03:08Z

Protected "7vsp" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:03, 3 November 2021

OCA: New page: '''Unreleased structure''' The entry 7vsp is ON HOLD Authors: Description: Category: Unreleased Structures

2021-11-03T13:03:07Z

New page: '''Unreleased structure''' The entry 7vsp is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7vsp is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

7vsp - Revision history

OCA at 11:18, 30 March 2022

OCA at 06:23, 2 March 2022

OCA at 12:28, 29 December 2021

OCA: Protected "7vsp" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7vsp is ON HOLD Authors: Description: Category: Unreleased Structures