7w0p - Revision history

OCA at 04:18, 8 September 2022

2022-09-08T04:18:36Z

←Older revision		Revision as of 04:18, 8 September 2022
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-	====	+	==Cryo-EM structure of a GPCR-Gi complex with peptide==
-	<StructureSection load='7w0p' size='340' side='right'caption='[[7w0p]]' scene=''>	+	<StructureSection load='7w0p' size='340' side='right'caption='[[7w0p]], [[Resolution\|resolution]] 3.16Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7w0p]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7W0P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7W0P FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7w0p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7w0p OCA], [https://pdbe.org/7w0p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7w0p RCSB], [https://www.ebi.ac.uk/pdbsum/7w0p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7w0p ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7w0p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7w0p OCA], [https://pdbe.org/7w0p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7w0p RCSB], [https://www.ebi.ac.uk/pdbsum/7w0p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7w0p ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/GNAI1_HUMAN GNAI1_HUMAN]] Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli. The inactive GDP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. May play a role in cell division.<ref>PMID:17635935</ref> <ref>PMID:17264214</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The technique of cryogenic-electron microscopy (cryo-EM) has revolutionized the field of membrane protein structure and function with a focus on the dominantly observed molecular species. This report describes the structural characterization of a fully active human apelin receptor (APJR) complexed with heterotrimeric G protein observed in both 2:1 and 1:1 stoichiometric ratios. We use cryo-EM single-particle analysis to determine the structural details of both species from the same sample preparation. Protein preparations, in the presence of the endogenous peptide ligand ELA or a synthetic small molecule, both demonstrate these mixed stoichiometric states. Structural differences in G protein engagement between dimeric and monomeric APJR suggest a role for the stoichiometry of G protein-coupled receptor- (GPCR-)G protein coupling on downstream signaling and receptor pharmacology. Furthermore, a small, hydrophobic dimer interface provides a starting framework for additional class A GPCR dimerization studies. Together, these findings uncover a mechanism of versatile regulation through oligomerization by which GPCRs can modulate their signaling.
		+
		+	Structural insight into apelin receptor-G protein stoichiometry.,Yue Y, Liu L, Wu LJ, Wu Y, Wang L, Li F, Liu J, Han GW, Chen B, Lin X, Brouillette RL, Breault E, Longpre JM, Shi S, Lei H, Sarret P, Stevens RC, Hanson MA, Xu F Nat Struct Mol Biol. 2022 Jul;29(7):688-697. doi: 10.1038/s41594-022-00797-5., Epub 2022 Jul 11. PMID:35817871<ref>PMID:35817871</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7w0p" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Escherichia coli]]
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Hanson M]]
		+	[[Category: Liu LE]]
		+	[[Category: Wu LJ]]
		+	[[Category: Xu F]]
		+	[[Category: Yue Y]]

OCA at 18:06, 27 July 2022

2022-07-27T18:06:30Z

←Older revision		Revision as of 18:06, 27 July 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7w0p ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='7w0p' size='340' side='right'caption='[[7w0p]]' scene=''>
-	~~Authors~~: ~~Xu, F~~.~~, Yue, Y~~.~~, Liu, L~~.E.~~, Wu, L~~.J.~~, Hanson, M~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~: ~~Cryo-EM structure of a GPCR-Gi complex with peptide~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7w0p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7w0p OCA], [https://pdbe.org/7w0p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7w0p RCSB], [https://www.ebi.ac.uk/pdbsum/7w0p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7w0p ProSAT]</span></td></tr>
-	[~~[Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[~~[Category~~: ~~Wu, L~~.J]]	+	__TOC__
-	[[~~Category~~: ~~Yue~~, Y]]	+	</StructureSection>
-	[~~[Category~~: Xu, F]]	+	[[Category: Large Structures]]
-	[[Category: ~~Liu, L.E~~]]	+	[[Category: Z-disk]]
-	[[Category: ~~Hanson, M~~]]	+

OCA at 12:33, 29 December 2021

2021-12-29T12:33:42Z

←Older revision		Revision as of 12:33, 29 December 2021
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7w0p is ON HOLD	+	The entry 7w0p is ON HOLD until Paper Publication

	Authors: Xu, F., Yue, Y., Liu, L.E., Wu, L.J., Hanson, M.		Authors: Xu, F., Yue, Y., Liu, L.E., Wu, L.J., Hanson, M.

OCA: Protected "7w0p" [edit=sysop:move=sysop]

2021-11-24T11:11:47Z

Protected "7w0p" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:11, 24 November 2021

OCA: New page: '''Unreleased structure''' The entry 7w0p is ON HOLD Authors: Xu, F., Yue, Y., Liu, L.E., Wu, L.J., Hanson, M. Description: Cryo-EM structure of a GPCR-Gi complex with peptide [[Catego...

2021-11-24T11:11:46Z

New page: '''Unreleased structure''' The entry 7w0p is ON HOLD Authors: Xu, F., Yue, Y., Liu, L.E., Wu, L.J., Hanson, M. Description: Cryo-EM structure of a GPCR-Gi complex with peptide [[Catego...

New page

'''Unreleased structure'''

The entry 7w0p is ON HOLD

Authors: Xu, F., Yue, Y., Liu, L.E., Wu, L.J., Hanson, M.

Description: Cryo-EM structure of a GPCR-Gi complex with peptide
[[Category: Unreleased Structures]]
[[Category: Wu, L.J]]
[[Category: Yue, Y]]
[[Category: Xu, F]]
[[Category: Liu, L.E]]
[[Category: Hanson, M]]