OCA at 17:35, 29 November 2023

2023-11-29T17:35:36Z

←Older revision		Revision as of 17:35, 29 November 2023
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 7w30 is ~~ON HOLD until Paper~~ Publication	+	==Tudor domain of SMN in complex with a small molecule==
		+	<StructureSection load='7w30' size='340' side='right'caption='[[7w30]], [[Resolution\|resolution]] 1.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[7w30]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7W30 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7W30 FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.8Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=8AT:1,2-dimethylquinolin-4-imine'>8AT</scene>, <scene name='pdbligand=UNX:UNKNOWN+ATOM+OR+ION'>UNX</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7w30 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7w30 OCA], [https://pdbe.org/7w30 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7w30 RCSB], [https://www.ebi.ac.uk/pdbsum/7w30 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7w30 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/SMN_HUMAN SMN_HUMAN]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Survival of motor neuron (SMN) functions in diverse biological pathways via recognition of symmetric dimethylarginine (Rme2s) on proteins by its Tudor domain, and deficiency of SMN leads to spinal muscular atrophy. Here we report a potent and selective antagonist with a 4-iminopyridine scaffold targeting the Tudor domain of SMN. Our structural and mutagenesis studies indicate that both the aromatic ring and imino groups of compound 1 contribute to its selective binding to SMN. Various on-target engagement assays support that compound 1 specifically recognizes SMN in a cellular context and prevents the interaction of SMN with the R1810me2s of RNA polymerase II subunit POLR2A, resulting in transcription termination and R-loop accumulation mimicking SMN depletion. Thus, in addition to the antisense, RNAi and CRISPR/Cas9 techniques, potent SMN antagonists could be used as an efficient tool to understand the biological functions of SMN.

-	~~Authors: Li~~, W., ~~Arrowsmith~~, C.H., ~~Bountra~~, C., ~~Edwards~~, ~~A.M.~~, ~~Liu~~, Y., ~~Min~~, J.	+	A small molecule antagonist of SMN disrupts the interaction between SMN and RNAP II.,Liu Y, Iqbal A, Li W, Ni Z, Wang Y, Ramprasad J, Abraham KJ, Zhang M, Zhao DY, Qin S, Loppnau P, Jiang H, Guo X, Brown PJ, Zhen X, Xu G, Mekhail K, Ji X, Bedford MT, Greenblatt JF, Min J Nat Commun. 2022 Sep 16;13(1):5453. doi: 10.1038/s41467-022-33229-5. PMID:36114190<ref>PMID:36114190</ref>

-	~~Description: Tudor domain of SMN in complex with~~ a ~~small molecule~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: ~~Min, J~~]]	+	<div class="pdbe-citations 7w30" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Edwards, A.M~~]]	+	== References ==
-	[[Category: ~~Arrowsmith,~~ C.H]]	+	<references/>
-	[[Category: ~~Liu, Y~~]]	+	__TOC__
-	[[Category: Li, W]]	+	</StructureSection>
-	[[Category: ~~Bountra, C~~]]	+	[[Category: Homo sapiens]]
		+	[[Category: Large Structures]]
		+	[[Category: Arrowsmith CH]]
		+	[[Category: Bountra C]]
		+	[[Category: Edwards AM]]
		+	[[Category: Li W]]
		+	[[Category: Liu Y]]
		+	[[Category: Min J]]

OCA at 08:43, 8 December 2021

2021-12-08T08:43:15Z

←Older revision		Revision as of 08:43, 8 December 2021
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7w30 is ON HOLD	+	The entry 7w30 is ON HOLD until Paper Publication

-	Authors: Li, W., Arrowsmith, C.H., Bountra, C., Edwards, A.M.	+	Authors: Li, W., Arrowsmith, C.H., Bountra, C., Edwards, A.M., Liu, Y., Min, J.

	Description: Tudor domain of SMN in complex with a small molecule		Description: Tudor domain of SMN in complex with a small molecule
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
		+	[[Category: Min, J]]
		+	[[Category: Edwards, A.M]]
	[[Category: Arrowsmith, C.H]]		[[Category: Arrowsmith, C.H]]
		+	[[Category: Liu, Y]]
	[[Category: Li, W]]		[[Category: Li, W]]
-	[[Category: Edwards, A.M]]
	[[Category: Bountra, C]]		[[Category: Bountra, C]]

OCA: Protected "7w30" [edit=sysop:move=sysop]

2021-12-01T05:20:39Z

Protected "7w30" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:20, 1 December 2021

OCA: New page: '''Unreleased structure''' The entry 7w30 is ON HOLD Authors: Li, W., Arrowsmith, C.H., Bountra, C., Edwards, A.M. Description: Tudor domain of SMN in complex with a small molecule [[C...

2021-12-01T05:20:38Z

New page: '''Unreleased structure''' The entry 7w30 is ON HOLD Authors: Li, W., Arrowsmith, C.H., Bountra, C., Edwards, A.M. Description: Tudor domain of SMN in complex with a small molecule [[C...

New page

'''Unreleased structure'''

The entry 7w30 is ON HOLD

Authors: Li, W., Arrowsmith, C.H., Bountra, C., Edwards, A.M.

Description: Tudor domain of SMN in complex with a small molecule
[[Category: Unreleased Structures]]
[[Category: Arrowsmith, C.H]]
[[Category: Li, W]]
[[Category: Edwards, A.M]]
[[Category: Bountra, C]]

7w30 - Revision history

OCA at 17:35, 29 November 2023

OCA at 08:43, 8 December 2021

OCA: Protected "7w30" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 7w30 is ON HOLD Authors: Li, W., Arrowsmith, C.H., Bountra, C., Edwards, A.M. Description: Tudor domain of SMN in complex with a small molecule [[C...