7wan - Revision history

OCA at 17:38, 29 November 2023

2023-11-29T17:38:27Z

←Older revision		Revision as of 17:38, 29 November 2023
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	<StructureSection load='7wan' size='340' side='right'caption='[[7wan]], [[Resolution\|resolution]] 2.28Å' scene=''>		<StructureSection load='7wan' size='340' side='right'caption='[[7wan]], [[Resolution\|resolution]] 2.28Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7wan]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7WAN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7WAN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7wan]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhodococcus_sp. Rhodococcus sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7WAN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7WAN FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=8MS:(R)-[4-(2-azanylhydrazinyl)phenyl]-[2-[2-(2-hexoxyethoxy)ethoxy]ethylamino]methanol'>8MS</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.284Å</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Haloalkane_dehalogenase Haloalkane dehalogenase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.8.1.5 3.8.1.5] </span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=8MS:(R)-[4-(2-azanylhydrazinyl)phenyl]-[2-[2-(2-hexoxyethoxy)ethoxy]ethylamino]methanol'>8MS</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7wan FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7wan OCA], [https://pdbe.org/7wan PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7wan RCSB], [https://www.ebi.ac.uk/pdbsum/7wan PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7wan ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7wan FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7wan OCA], [https://pdbe.org/7wan PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7wan RCSB], [https://www.ebi.ac.uk/pdbsum/7wan PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7wan ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/DHAA_RHOSO DHAA_RHOSO]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 7wan" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 7wan" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Dehalogenase 3D structures\|Dehalogenase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Haloalkane dehalogenase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Kang, M]]	+	[[Category: Rhodococcus sp]]
-	[[Category: Lee, C]]	+	[[Category: Kang M]]
-	[[Category: Lee, H]]	+	[[Category: Lee C]]
-	[[Category: Pratyush, M]]	+	[[Category: Lee H]]
-	[[Category: Rhee, H]]	+	[[Category: Pratyush M]]
-	~~[[Category: Halotag]]~~	+	[[Category: Rhee H]]
-	~~[[Category: Hydrolase~~]]	+

OCA at 07:28, 9 March 2022

2022-03-09T07:28:20Z

←Older revision		Revision as of 07:28, 9 March 2022
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	==Crystal structure of HaloTag complexed with UL2==		==Crystal structure of HaloTag complexed with UL2==
-	<StructureSection load='7wan' size='340' side='right'caption='[[7wan]]' scene=''>	+	<StructureSection load='7wan' size='340' side='right'caption='[[7wan]], [[Resolution\|resolution]] 2.28Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7WAN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7WAN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7wan]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7WAN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7WAN FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7wan FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7wan OCA], [https://pdbe.org/7wan PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7wan RCSB], [https://www.ebi.ac.uk/pdbsum/7wan PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7wan ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=8MS:(R)-[4-(2-azanylhydrazinyl)phenyl]-[2-[2-(2-hexoxyethoxy)ethoxy]ethylamino]methanol'>8MS</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Haloalkane_dehalogenase Haloalkane dehalogenase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.8.1.5 3.8.1.5] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7wan FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7wan OCA], [https://pdbe.org/7wan PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7wan RCSB], [https://www.ebi.ac.uk/pdbsum/7wan PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7wan ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	We developed a proximity photo-crosslinking method (Spotlight) with a 4-azido-N-ethyl-1,8-naphthalimide (AzNP) moiety that can be converted to reactive aryl nitrene species using ambient blue light-emitting diode light. Using an AzNP-conjugated HaloTag ligand (VL1), blue light-induced photo-crosslinked products of various HaloTag-conjugated proteins of interest were detected in subcellular spaces in live cells. Chemical or heat stress-induced dynamic changes in the proteome were also detected, and photo-crosslinking in the mouse brain tissue was enabled. Using Spotlight, we further identified the host interactome of SARS-CoV-2 nucleocapsid (N) protein, which is essential for viral genome assembly. Mass analysis of the VL1-crosslinked product of N-HaloTag in HEK293T cells showed that RNA-binding proteins in stress granules were exclusively enriched in the cross-linked samples. These results tell that our method can reveal the interactome of protein of interest within a short distance in live cells.
		+
		+	A chemical tool for blue light-inducible proximity photo-crosslinking in live cells.,Mishra PK, Kang MG, Lee H, Kim S, Choi S, Sharma N, Park CM, Ko J, Lee C, Seo JK, Rhee HW Chem Sci. 2021 Dec 10;13(4):955-966. doi: 10.1039/d1sc04871f. eCollection 2022, Jan 26. PMID:35211260<ref>PMID:35211260</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7wan" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Haloalkane dehalogenase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Kang M]]	+	[[Category: Kang, M]]
-	[[Category: Lee C]]	+	[[Category: Lee, C]]
-	[[Category: Lee H]]	+	[[Category: Lee, H]]
-	[[Category: Pratyush M]]	+	[[Category: Pratyush, M]]
-	[[Category: Rhee H]]	+	[[Category: Rhee, H]]
		+	[[Category: Halotag]]
		+	[[Category: Hydrolase]]

OCA at 07:05, 2 February 2022

2022-02-02T07:05:10Z

←Older revision		Revision as of 07:05, 2 February 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7wan is ~~ON HOLD until Paper Publication~~	+	==Crystal structure of HaloTag complexed with UL2==
-		+	<StructureSection load='7wan' size='340' side='right'caption='[[7wan]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7WAN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7WAN FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7wan FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7wan OCA], [https://pdbe.org/7wan PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7wan RCSB], [https://www.ebi.ac.uk/pdbsum/7wan PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7wan ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Kang M]]
		+	[[Category: Lee C]]
		+	[[Category: Lee H]]
		+	[[Category: Pratyush M]]
		+	[[Category: Rhee H]]

OCA at 08:43, 12 January 2022

2022-01-12T08:43:59Z

←Older revision		Revision as of 08:43, 12 January 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7wan is ON HOLD	+	The entry 7wan is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "7wan" [edit=sysop:move=sysop]

2021-12-22T14:16:05Z

Protected "7wan" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:16, 22 December 2021

OCA: New page: '''Unreleased structure''' The entry 7wan is ON HOLD Authors: Description: Category: Unreleased Structures

2021-12-22T14:16:04Z

New page: '''Unreleased structure''' The entry 7wan is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 7wan is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]