7wi4 - Revision history

OCA at 05:23, 15 June 2022

2022-06-15T05:23:55Z

←Older revision		Revision as of 05:23, 15 June 2022
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	==Cryo-EM structure of E.Coli FtsH protease cytosolic domains==		==Cryo-EM structure of E.Coli FtsH protease cytosolic domains==
-	<StructureSection load='7wi4' size='340' side='right'caption='[[7wi4]]' scene=''>	+	<StructureSection load='7wi4' size='340' side='right'caption='[[7wi4]], [[Resolution\|resolution]] 3.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7WI4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7WI4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7wi4]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7WI4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7WI4 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7wi4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7wi4 OCA], [https://pdbe.org/7wi4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7wi4 RCSB], [https://www.ebi.ac.uk/pdbsum/7wi4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7wi4 ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7wi4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7wi4 OCA], [https://pdbe.org/7wi4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7wi4 RCSB], [https://www.ebi.ac.uk/pdbsum/7wi4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7wi4 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/FTSH_ECOLI FTSH_ECOLI]] Acts as a processive, ATP-dependent zinc metallopeptidase for both cytoplasmic and membrane proteins. Plays a role in the quality control of integral membrane proteins. Degrades a few membrane proteins that have not been assembled into complexes such as SecY, F(0) ATPase subunit a and YccA, and also cytoplasmic proteins sigma-32, LpxC, KdtA and phage lambda cII protein among others. Degrades membrane proteins in a processive manner starting at either the N- or C-terminus; recognition requires a cytoplasmic tail of about 20 residues with no apparent sequence requirements. It presumably dislocates membrane-spanning and periplasmic segments of the protein into the cytoplasm to degrade them, this probably requires ATP. Degrades C-terminal-tagged cytoplasmic proteins which are tagged with an 11-amino-acid nonpolar destabilizing tail via a mechanism involving the 10SA (SsrA) stable RNA.<ref>PMID:7781608</ref> <ref>PMID:7753838</ref> <ref>PMID:10048027</ref> <ref>PMID:18776015</ref> As FtsH regulates the levels of both LpxC and KdtA it is required for synthesis of both the protein and lipid components of lipopolysaccharide (LPS).<ref>PMID:7781608</ref> <ref>PMID:7753838</ref> <ref>PMID:10048027</ref> <ref>PMID:18776015</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The membrane-bound AAA protease FtsH is the key player controlling protein quality in bacteria. Two single-pass membrane proteins, HflK and HflC, interact with FtsH to modulate its proteolytic activity. Here, we present structure of the entire FtsH-HflKC complex, comprising 12 copies of both HflK and HflC, all of which interact reciprocally to form a cage, as well as four FtsH hexamers with periplasmic domains and transmembrane helices enclosed inside the cage and cytoplasmic domains situated at the base of the cage. FtsH K61/D62/S63 in the beta2-beta3 loop in the periplasmic domain directly interact with HflK, contributing to complex formation. Pull-down and in vivo enzymatic activity assays validate the importance of the interacting interface for FtsH-HflKC complex formation. Structural comparison with the substrate-bound human m-AAA protease AFG3L2 offers implications for the HflKC cage in modulating substrate access to FtsH. Together, our findings provide a better understanding of FtsH-type AAA protease holoenzyme assembly and regulation.
		+
		+	Cryo-EM structure of the entire FtsH-HflKC AAA protease complex.,Qiao Z, Yokoyama T, Yan XF, Beh IT, Shi J, Basak S, Akiyama Y, Gao YG Cell Rep. 2022 May 31;39(9):110890. doi: 10.1016/j.celrep.2022.110890. PMID:35649372<ref>PMID:35649372</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7wi4" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Gao YG]]	+	[[Category: Gao, Y G]]
-	[[Category: Qiao Z]]	+	[[Category: Qiao, Z]]
		+	[[Category: Complex]]
		+	[[Category: Hydrolase]]
		+	[[Category: Membrane protein]]

OCA at 05:37, 8 June 2022

2022-06-08T05:37:35Z

←Older revision		Revision as of 05:37, 8 June 2022
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-	'''Unreleased structure'''

-	~~The entry~~ 7wi4 ~~is ON HOLD until Paper Publication~~	+	==Cryo-EM structure of E.Coli FtsH protease cytosolic domains==
-		+	<StructureSection load='7wi4' size='340' side='right'caption='[[7wi4]]' scene=''>
-	~~Authors~~: ~~Qiao~~, Z., ~~Gao~~, Y.G.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7WI4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7WI4 FirstGlance]. <br>
-	~~Description~~: ~~Cryo~~-~~EM structure of E~~.~~Coli FtsH protease cytosolic domains~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7wi4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7wi4 OCA], [https://pdbe.org/7wi4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7wi4 RCSB], [https://www.ebi.ac.uk/pdbsum/7wi4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7wi4 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: Gao~~, Y.G~~]]	+	__TOC__
-	[[Category: Qiao, Z]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Gao YG]]
		+	[[Category: Qiao Z]]

OCA at 06:42, 25 May 2022

2022-05-25T06:42:47Z

←Older revision		Revision as of 06:42, 25 May 2022
Line 5:		Line 5:
	Authors: Qiao, Z., Gao, Y.G.		Authors: Qiao, Z., Gao, Y.G.

-	Description: Cryo-EM structure of E.~~coli membrane protein complex~~	+	Description: Cryo-EM structure of E.Coli FtsH protease cytosolic domains
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Qiao, Z]]
	[[Category: Gao, Y.G]]		[[Category: Gao, Y.G]]
		+	[[Category: Qiao, Z]]

OCA at 09:16, 16 February 2022

2022-02-16T09:16:07Z

←Older revision		Revision as of 09:16, 16 February 2022
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7wi4 is ON HOLD	+	The entry 7wi4 is ON HOLD until Paper Publication

	Authors: Qiao, Z., Gao, Y.G.		Authors: Qiao, Z., Gao, Y.G.
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	Description: Cryo-EM structure of E.coli membrane protein complex		Description: Cryo-EM structure of E.coli membrane protein complex
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Gao, Y.G]]
	[[Category: Qiao, Z]]		[[Category: Qiao, Z]]
		+	[[Category: Gao, Y.G]]

OCA: Protected "7wi4" [edit=sysop:move=sysop]

2022-01-12T08:45:18Z

Protected "7wi4" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:45, 12 January 2022

OCA: New page: '''Unreleased structure''' The entry 7wi4 is ON HOLD Authors: Qiao, Z., Gao, Y.G. Description: Cryo-EM structure of E.coli membrane protein complex Category: Unreleased Structures ...

2022-01-12T08:45:16Z

New page: '''Unreleased structure''' The entry 7wi4 is ON HOLD Authors: Qiao, Z., Gao, Y.G. Description: Cryo-EM structure of E.coli membrane protein complex Category: Unreleased Structures ...

New page

'''Unreleased structure'''

The entry 7wi4 is ON HOLD

Authors: Qiao, Z., Gao, Y.G.

Description: Cryo-EM structure of E.coli membrane protein complex
[[Category: Unreleased Structures]]
[[Category: Gao, Y.G]]
[[Category: Qiao, Z]]