7wt0 - Revision history

OCA at 17:44, 29 November 2023

2023-11-29T17:44:20Z

←Older revision		Revision as of 17:44, 29 November 2023
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	<StructureSection load='7wt0' size='340' side='right'caption='[[7wt0]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='7wt0' size='340' side='right'caption='[[7wt0]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[7wt0]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7WT0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7WT0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7wt0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7WT0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7WT0 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5ZO:(~{E})-3-(1,3-benzothiazol-2-yl)-4-(4-methoxyphenyl)but-3-enoic+acid'>5ZO</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Lactoylglutathione_lyase Lactoylglutathione lyase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.4.1.5 4.4.1.5] </span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5ZO:(~{E})-3-(1,3-benzothiazol-2-yl)-4-(4-methoxyphenyl)but-3-enoic+acid'>5ZO</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7wt0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7wt0 OCA], [https://pdbe.org/7wt0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7wt0 RCSB], [https://www.ebi.ac.uk/pdbsum/7wt0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7wt0 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7wt0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7wt0 OCA], [https://pdbe.org/7wt0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7wt0 RCSB], [https://www.ebi.ac.uk/pdbsum/7wt0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7wt0 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/LGUL_HUMAN LGUL_HUMAN]] Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione. Involved in the regulation of TNF-induced transcriptional activity of NF-kappa-B.<ref>PMID:19199007</ref>	+	[https://www.uniprot.org/uniprot/LGUL_HUMAN LGUL_HUMAN] Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione. Involved in the regulation of TNF-induced transcriptional activity of NF-kappa-B.<ref>PMID:19199007</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 7wt0" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 7wt0" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Glyoxalase 3D structures\|Glyoxalase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Lactoylglutathione lyase~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Usami, M]]	+	[[Category: Usami M]]
-	[[Category: Yokoyama, H]]	+	[[Category: Yokoyama H]]
-	~~[[Category: Glyoxalase i]]~~	+
-	~~[[Category: Lyase]]~~	+
-	~~[[Category: Zinc metalloenzyme~~]]	+

OCA at 07:35, 29 June 2022

2022-06-29T07:35:37Z

←Older revision		Revision as of 07:35, 29 June 2022
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	==human glyoxalase I (with C-ter His tag) in complex with TLSC702==		==human glyoxalase I (with C-ter His tag) in complex with TLSC702==
-	<StructureSection load='7wt0' size='340' side='right'caption='[[7wt0]]' scene=''>	+	<StructureSection load='7wt0' size='340' side='right'caption='[[7wt0]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7WT0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7WT0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[7wt0]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7WT0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7WT0 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7wt0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7wt0 OCA], [https://pdbe.org/7wt0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7wt0 RCSB], [https://www.ebi.ac.uk/pdbsum/7wt0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7wt0 ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5ZO:(~{E})-3-(1,3-benzothiazol-2-yl)-4-(4-methoxyphenyl)but-3-enoic+acid'>5ZO</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Lactoylglutathione_lyase Lactoylglutathione lyase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.4.1.5 4.4.1.5] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7wt0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7wt0 OCA], [https://pdbe.org/7wt0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7wt0 RCSB], [https://www.ebi.ac.uk/pdbsum/7wt0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7wt0 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/LGUL_HUMAN LGUL_HUMAN]] Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione. Involved in the regulation of TNF-induced transcriptional activity of NF-kappa-B.<ref>PMID:19199007</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Human glyoxalase I (hGLO I) is an enzyme for detoxification of methylglyoxal (MG) and has been considered an attractive target for the development of new anticancer drugs. In our previous report, the GLO I inhibitor TLSC702 induced apoptosis in tumor cells. Here, we determined the crystal structures of hGLO I and its complex with TLSC702. In the complex, the carboxyl O atom of TLSC702 is coordinated to Zn(2+) , and TLSC702 mainly shows van der Waals interaction with hydrophobic residues. In the inhibitor-unbound structure, glycerol, which has similar functional groups to MG, was bound to Zn(2+) , indicating that GLO I can easily bind to MG. This study provides a structural basis to develop better anticancer drugs.
		+
		+	Crystal structures of human glyoxalase I and its complex with TLSC702 reveal inhibitor binding mode and substrate preference.,Usami M, Ando K, Shibuya A, Takasawa R, Yokoyama H FEBS Lett. 2022 Apr 1. doi: 10.1002/1873-3468.14344. PMID:35363883<ref>PMID:35363883</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 7wt0" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Lactoylglutathione lyase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Usami M]]	+	[[Category: Usami, M]]
-	[[Category: Yokoyama H]]	+	[[Category: Yokoyama, H]]
		+	[[Category: Glyoxalase i]]
		+	[[Category: Lyase]]
		+	[[Category: Zinc metalloenzyme]]

OCA at 09:49, 13 April 2022

2022-04-13T09:49:42Z

←Older revision		Revision as of 09:49, 13 April 2022
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-	'''Unreleased structure'''

-	~~The entry 7wt0 is ON HOLD until Feb 03 2024~~	+	==human glyoxalase I (with C-ter His tag) in complex with TLSC702==
-		+	<StructureSection load='7wt0' size='340' side='right'caption='[[7wt0]]' scene=''>
-	~~Authors: Usami, M., Yokoyama, H.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7WT0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7WT0 FirstGlance]. <br>
-	~~Description:~~ human glyoxalase I (with C-ter His tag) in complex with TLSC702	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7wt0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7wt0 OCA], [https://pdbe.org/7wt0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7wt0 RCSB], [https://www.ebi.ac.uk/pdbsum/7wt0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7wt0 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: Usami, M]]	+	__TOC__
-	[[Category: Yokoyama, H]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Usami M]]
		+	[[Category: Yokoyama H]]

OCA at 06:38, 23 February 2022

2022-02-23T06:38:19Z

←Older revision		Revision as of 06:38, 23 February 2022
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 7wt0 is ON HOLD	+	The entry 7wt0 is ON HOLD until Feb 03 2024

	Authors: Usami, M., Yokoyama, H.		Authors: Usami, M., Yokoyama, H.
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	Description: human glyoxalase I (with C-ter His tag) in complex with TLSC702		Description: human glyoxalase I (with C-ter His tag) in complex with TLSC702
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Yokoyama, H]]
	[[Category: Usami, M]]		[[Category: Usami, M]]
		+	[[Category: Yokoyama, H]]

OCA: Protected "7wt0" [edit=sysop:move=sysop]

2022-02-09T06:29:15Z

Protected "7wt0" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:29, 9 February 2022

OCA: New page: '''Unreleased structure''' The entry 7wt0 is ON HOLD Authors: Usami, M., Yokoyama, H. Description: human glyoxalase I (with C-ter His tag) in complex with TLSC702 [[Category: Unrelease...

2022-02-09T06:29:14Z

New page: '''Unreleased structure''' The entry 7wt0 is ON HOLD Authors: Usami, M., Yokoyama, H. Description: human glyoxalase I (with C-ter His tag) in complex with TLSC702 [[Category: Unrelease...

New page

'''Unreleased structure'''

The entry 7wt0 is ON HOLD

Authors: Usami, M., Yokoyama, H.

Description: human glyoxalase I (with C-ter His tag) in complex with TLSC702
[[Category: Unreleased Structures]]
[[Category: Yokoyama, H]]
[[Category: Usami, M]]